Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

Effects of high Cell Density on growth-Associated Monoclonal Antibody Production by Hybridoma T0405 Cells Immobilized in Macroporous Cellulose carriers

  • Hideki Mochoda (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodi, Tsukuba city 305-8572, Japen) ;
  • Wang, Pi-Chao (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodi, Tsukuba city 305-8572, Japen) ;
  • Fr Jr. Nayve (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodi, Tsukuba city 305-8572, Japen) ;
  • Ryuji Sato (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodi, Tsukuba city 305-8572, Japen) ;
  • Minoru Harige (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodi, Tsukuba city 305-8572, Japen) ;
  • Nakao Nomura (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodi, Tsukuba city 305-8572, Japen) ;
  • Masatoshi Matsumura (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tennodi, Tsukuba city 305-8572, Japen)
  • Published : 2000.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Relationship between monoclonal antibody (MAb) productivity and growth rate, and effects of high cell density on MAb production rate increased with increasing specifis growth rate in both suspended and immobilized continuous cultures indicate a positively growth-associated relationship between MAb productivity and growth rate. moreover, the specific production rate was higher in the immobilized cell culture than that in suspended one at all dilution rates. In order to clarify these phenomana, MAb mPNA experession and cell cycle distribution were investigated in bacth cultures with immobilized cells and suspended cells. RT-PCR was used for observation of MAb mRNA expression and a two-color bromodeoxyuridine (BrdU)/propidium iodide (PI) flow cytometry method for determination of cell cycle distribution. The results revealed that MAb nRNA expression until dead phase, which was longer than in suspended cell. The cell cycle distribution patterns were observed almost the same for both immobilized and suspended cells. Such results may imply that a high cell density state has positive influence on the mRNA expression and on growth-associated Mab productivity of T0405 cells.

Keywords

References

  1. Nature v.216 Growth of cell-strains and primary cells on microcarriers in homogeous culture van Wezel, A. L.
  2. Biotechnol. Bioeng. v.38 Production of monoclonal antiboby using free suspended and immobilized hybridoma cells: Effects of serum Lee, G. M.;A. Varma;B. O. Palsson
  3. Biotechnol. Bioeng. v.36 Immobilization can improve the stability of hybridoma antibody productivity in serum media Lee, G. M.;B. O. Palsson
  4. Animal Cell Technol.: Basic & Applied Aspects v.8 Monoclonal antibody production using macroporous microcarrier Yanagida, K.;K. Shudo;J. Shirokaze
  5. Animal Cell Technol.: Basic & Applied Aspects v.8 Physiological status of immobilized hybridoma cells: Assessment through extracellular DNA analysis Sramkova, K.;F. Franek;I. Ludemann;R. Portner
  6. Appl. Microbiol. Biotechnol. v.37 Growth and death behaviour of anchorage-independent animal cells immobilized within porous support matrices Yamaji, H.;H. Fukuda
  7. Animal Cell Technol.: Basic & Applied Aspects v.5 Medium optimization for perfused culture to provide high product titer Ray, N. G.;S. S. Ozturk;A. S. Tung;H. L. Pang;M. W. Young;P. W. Runstadler
  8. Enzyme Microb. Technol. v.14 Kinetic studies of cellular metabolic activity, specific IgG production rate, IgG mRNA stability and accumulation during hybridoma batch culture Leno, M.;O. W. Merten;J. Hache
  9. Biotechnol. Bioeng. v.38 Kinetics of recombinant immunoglobilin production by mammalian cells in continuous culture Robinson, D. K.;K. W. Memmert
  10. Biotechnol. Prog. v.7 Manipulation of heterogenous hybridoma cultures for overproduction of monoclonal antibodies Mckinney, K. L.;R. Dilkwith;G. Belfort
  11. Enzyme Microb. Technol. v.14 Relationship between hybridoma growth and monoclonal antibody production Hayter, P. M.;N. F. Kirkby;R. E. Spier
  12. Can. J. Chem. Eng. v.69 The effect of specific rate and death rate on monoclonal antibody production in hybridoma chemostat culture Linalds, T. I.;N. Kalogeracs;L. A. Behie;L. R. Lamontagne
  13. Biotechnol. Tech. v.10 Improved MTT assay for the measurement of viable animal cell number in porous cellulose carriers Nomura, N.;K. Saijo;M. Kato;P. C. Wang;T. Ohno;M. Matsumura
  14. Proc. Natl. Acad. Sci. USA v.80 Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine Dolbeare, F.;H. Gratzner;M. G. Pallavicini;J. W. Gray
  15. Flow cytometry and sorting Quantitative cell cycle analysis Gray, J. W.;F. Dolbeare;M. G. Pallavicini;M. R. Melamed(ed.);T. Lindmo(ed.);M. L. Mendelsohn(ed.)
  16. Gene v.121 Cloning and characterization of cDNAs coding for heavy and light chains of a monoclonal antibody specific for pre-S2 antigen of hepatitis B virus Hong, H. J.;A. K. Kim;C. J. Ryu;S. S. Park;H. K. Chung;K. S. Kwon;K. L. Kim;J. Kim;M. H. Han
  17. Proc. Natl. Acad. Sci. USA v.89 Human combinatorial antibody libraries to hepatitis B surface antigen Zebedee, S. L.;C. F. Ⅲ. Barbas;Y. L. Hom;R. H. Caothien;R. Graff;J. DeGraw;J. Pyati;R. LaPolla;D. R. Burton;R. A. Lerner;G. B. Thornton
  18. Mol. Immunol. v.27 Construction, expression and characterization of a murine/human chimeric antibody with specificity for hepatitis B surface antigen Li, Y. W.;D. K. Lawrie;P. Thammana;G. P. Moore;C. W. Shearman
  19. Mol. Immunol. v.30 Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus Jin, B. R.;C. J. Ryu;S. S. Park;U. Namgung;H. J. Hong;M. H. Han
  20. Biochim. Biophys. Acta v.1380 Cloning and sequence analysis of cDNAs encoding the heavy and light chain variable regions of a human monoclonal antibody with specificity for hepatitis B surface antigen Ryu, C. J.;B. R. Jin;H. K. Chung;H. J. Hong
  21. J. Immunol. Meth. v.192 Construction of recombinant extended single chain antibody peptide conjugates for use in the diagnosis of HIV-1 and HIV-1 Coia, G.;P. J. Hudson;G. G. Lilley
  22. Mol. Immunol. v.30 Cloning, expression and characterization of a murine-human chimeric antibody with specificity for pre-S2 surface antigen of hepatitis B virus Jin, B. R.;C. J. Ryu;S. S. Park;U. Namgung;H. J. Hong;M. H. Han
  23. J. Exp. Med. v.177 Mimicry of the alpha determinant of hepatitis B surface antigen by an antiidiotypic antibody. I. evaluation in hepatitis B surface antigen responder and nonresponder strains Pride, M. W.;A. Thakur;Y. Thanavala
  24. J. Biotechnol. v.34 Kinetic study on HBs-MAb production in continuous cultivation Harigae, M.;M. Matsumura;H. Kataoka
  25. Biotechnol. Bioeng. v.31 A study of hybridoma cell growth and antibody production kinetics in continuous culture Low, K. S.;C. Harbour;J. P. Barford
  26. J. Biotechnol. v.34 HBs-MAb production in perfusion culture with selective ammonia removal system Nayve, F. R. Jr.;T. Misato;M. Matsumura;H. Kataoka
  27. Biotechnol. Bioeng. v.41 Cell culture conditions determine the enhancement of specific monoclonal antibody productivity of calcium alginate-entrapped S3H5/γ2bA2 hybridoma cells Lee, G. M.;A. S. Varma;B. O. Palsson
  28. Animal Cell Technol.: Basic & Applied Aspects v.5 Biosynthesis of protein products by animal cells - are growth and non-growth associated concepts valid or useful? Barford, J. P.;P. J. Phillips;C. P. Marquis;C. Harbour
  29. J. Immunol. v.103 Immunoglobulin production in synchronized cultures of human hematopoietic cell lines Takahashi, M.;Y. Yagi;G. E. Moore;D. Pressman
  30. Biotech-nol. Bioeng. v.37 A structured model for monoclonal antibody synthesis in exponentially growing and stationary phase hybridoma cells Bibila, T.;M. C. Flickinger
  31. J. Molecular Biol. v.82 Stability of cytoplasmic ribonucleic acid in a mouse myeloma: Estimation of the half-life of the messenger RNA coding for an immunoglobulin light chain Cowan, N. J.;C. Milstein
  32. Biotechnol. Bioeng. v.32 A kinetic analysis of hybridoma growth and metabolism in batch and continuous suspension culture: Effect of nutrient concentration, dilution rate Miller, W. M.;H. W. Blanch;C. R. Wike
  33. Enzyme Technol. v.12 Coiled tube membrane bioreactor for cultivation of hybridoma cells producing monoclonal antibodies Hagedorn, J.;F. Kargi
  34. Animal Cell Technol.: Basic & Applied Aspects v.5 Growth control of hybridoma cultures by adding or depriving interleukin-6, caffeine, amino acids, growth factors, or antisense C myc Szuki, E.;K. Takahashi;S. Terada;F. Makishima
  35. Animal Cell Technol. : Basic & Applied Aspects v.5 Kinetics of cell metabolism and antibody production in high concentration perfusion cultures Zhang, S.;A. Handa-Corrigan;R. E. Spier
  36. Appl. Microbiol. Biotechnol. v.29 Oxygen uptake rate of immobilized growing hybridoma cells Shirai, Y.;K. Hashimoto;H. Yamaji;H. Kawahara
  37. Animal Cell Technol.: Basic & Applied Aspects v.5 Growth yields of hybridoma cells and monoclonal antibody production in high density culture Shirai, Y.;K. Hashimoto;T. Aoki;T. Yoshimi
  38. Trends in animal cell culture technology Shirai, Y., K. Hashimoto, and H. Akamatsu;H. Murakami(ed.)
  39. Ann. NY Acad. Sci. v.557 Hybridoma growth factor Aarden, L. A.
  40. Nature v.324 Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin Hirano, T.;K. Yasukawa;H. Harada;T. Taga;Y. Watanabe;T. Matsuda;S. Kashiwamura;K. Nakajima;K. Koyama;A. Iwamatsu;S. Tsunasawa;F. Sakiyama;H. Matsui;Y. Takahara;T. Taniguchi;T. Kishimoto