Biotechnology and Bioprocess Engineering:BBE

  • 제5권2호
  • /
  • Pages.99-105
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  • 2000
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Effects od Segree of Cell-Cell Contact on Liver Specific Function of Rat Primary Hepatocytes

  • Tang, Sung-Mun (Department of Chemical Engineering, Dongguk University, Seoul 100-715) ;
  • Lee, Doo-Hoon (Department of Chemical Engineering, Dongguk University, Seoul 100-715) ;
  • Park, Jung-Keug (Department of Chemical Engineering, Dongguk University, Seoul 100-715)
  • 발행 : 2000.04.01
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초록

Cell-Cell interaction and the extracellular matrix (ECM) are belisved to play essential roles during in vitro culturing of primary hepatocytes in the control of differentiation and in the maintenance of tissue spcific functions. The objective of this study was to examine the effects of degree of cell-cell contact (DCC) on liver sperific function of rat promary hepatocytes. Hepatocyte aggregates with various with various degrees of cell-cell contantact, I. e., dispersed cell, longish aggregate, rugged aggregate, and smooth spheroid were obtained at 1, 5-6, 15-20, and 36-48 hrs, respectively in suspension cultures grown in spinner flasks embedded in Caalginate bead and collagen gel in order. The may result from mass transfer limitation and shear damage caused by agitation during aggregation. The rugged aggregate showed a higer viability and albumin secretion rate than the dispersed cells or the other aggregates. This result indicates the possible enhancement of a bioartificial liver's (BAL) performance using primary hepatocytes and the reduction in time to prepare a BAL through optimization of the immobilization time.

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