Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Immortalization of Rat Kidney Glomerular Mesangial Cell and Its Coculture with Glomerular Epithelial Cell

  • Toshinobu Kida (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tsukaba City 305-8572, Japen) ;
  • Sachi Fujishima (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tsukaba City 305-8572, Japen) ;
  • Masatoshi Matsumra (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tsukaba City 305-8572, Japen) ;
  • Wang, Pi-Chao (Institute of Applied Biochemistry, Tsukuba University, 1-1-1 Tsukaba City 305-8572, Japen)
  • Published : 2000.04.01
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Abstract

Mesangial cell has several key roles in thee control of glomerular function: it partocipates in the regulation of glomerular filtration rate, macromolecular clearance, and as both a source and target of numerous hormones and autocrines. Many of these insights into mesangial cell function have been obtained by studying mesangial cells in culture. However, no suitble cell lines have established yet. We here reported the immortalization of rat kidney glomeruar mesangial cell by transfection of E6 and E7 genes of human papillomavirus type 16 (HPV-16) via electroporation and lipofection. The reslts showed that only electroporation could transfect the genes to mesangial cells and the transfected cells maintained the viability for longer than 6 months. Fluorescence microscopic observation showed that cellular contractility and phagocytosis, which are the two main phenotypes of mesangial cells with rat glomerular epithelial cells showed that the growth of mesangial dells was suppressed by epithelial cell, but the growth of epithelisl cells was enhanced by mesangial cells. Moreover, Such results may imply that the glomerular cell-cell interaction plays an important role in the regulation of cell proliferation and differentiation.

Keywords

References

  1. Kidney Int. v.17 The glomerular mesangium Michael, A. F.;W. F. Keane;L. Raij;R. L. Vernier;S. M. Mauer
  2. J. Clin. Invest. v.57 Angiotensin Ⅱ effects upon the glomerular micorcirculation and ultrafiltration coefficient of the rat Blantz, R. C.;K. S. Konnen;B. J. Tuchker
  3. FASEB J. v.1 The glomerular mesangial cell: an expanding role for a specialized pericyte Schlondorff, D.
  4. vitro. Kidney Int. v.35 Monocyte secretes factors that regulate glycosaminoglycan (GAG) synthesis by mesangial cells (MCs) Narita, I.;T. Morioka;K. Yoshida;F. Shimizu;M. Arakawa
  5. Kidney Int. v.38 Interleukin 6 is an autocrine growth factor for mesangial cells Ruef, C.;K. Budde;J. Lacy;W. Northemann;M. Baumann;R. B. Sterzel;D. L. Colema
  6. J. Clin. Invest. v.65 Construction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin Ⅱ and arginine vasopressin Ausiello, D.;J. J. Kreisberg;C. Roy;M. J. Karnovsky
  7. Physiol. Review v.69 Physiology of the mesangial cell Mene, P.;M. S. Simonson;M. J. Dunn
  8. Semin Nephro. v.2 Mesangial proliferative glomerulonephritis Cohen, A. H.;W. A. Border
  9. Lab. Invest. v.52 Mesangium and glomerular disease Kashgarian, M.
  10. J. Immunol. v.131 Stimulation of rat mesangial cell proliferation by macrophage Interleukin 1 Lovett, D. H.;J. L. Ryan;R. B. Sterzel
  11. Clin. Exp. Immunol. v.83 The production, by cultured human monocytes, of mesangial cell proliferation factors differeing from Interleukin 1 and Interleukin 6 Morioka, T.;I. Narita;F. Shimizu;Y. Oite
  12. Am. J. Pathol. v.139 Molulation of mesangial cell profiferation by endothelial cells in coculture Saeki, T.;T. Morioka;M. Arakawa;F. Shimizu;J. Oite
  13. Kidney Int. v.33 Glomerular epithelial, mesangial, and endothelial cell lines from transgenic mice MacKay, K.;L. J. Strikeer;S. Elliot;C. A. Pinkert;R. L. Brinster;G. E. Striker
  14. EMBO J. v.8 HPV16 E6 and E7 proteins cooperate to immortalize human skin keratinocytes Hawley-Nelson, P.;K. H. Vousden;N. L. Hubbert;D. R. Lowy;J. T. Schiller
  15. Science v.248 The human papillomavirus-16 E7 oncoprotein is able to bind the retinoblastoma gene product Dyson, N.;P. M. Howley;K. Munger;E. Harlow
  16. Science v.248 Association of human papillomavirus type 16 and 18 E6 proteins with p53 Werness, B. A.;A. J. Levine;P. M. Howley
  17. Kidney Int. v.25 Cyclic AMP-associated shape change in mesangial cells and its reversal by prostaglandin E2 Kreisberg, J. I.;M. A. Venkatachalan;P. Y. Patel
  18. Cell Struct. Funct. v.13 Establishment and characterization of an epithelial cell line SGE1, from isolated rat renal glomeruli Yamada, M.;M. Kawaguchi;H. Takamiya;H. Wada;T. Okigaki
  19. J. Gen. Virol. v.2 Induction of chromosome abnormalities in mouse and human epidermal keratinocytes by the human papillomavirus type 16 E7 oncogene Hashida, T.;S. Yamamoto
  20. Molecular Cloning: a Laboratory Manual Sambrook, J.;E. Fritsch;T. Maniatis
  21. Investi. Cell Pathol. v.2 Tissue culture of normal rat glomeruli: Isolation and morphological characterization of two homogeneous cell lines Foidart, J. B.;C. A. Dechenne;P. R. Mahieu;C. E. Creutz;J. Demeg
  22. Kidney Int. v.41 Long.term effects of morphine on mesangial cell proliferation and matrix synthesis Singhal, P. C.;N. Gibbons;M. Abramovici
  23. J. Immunol. v.130 Mechanism of suppression of mouse mesangial cell proliferation by macrophage supernatants Ooi, Y. M.;M. A. Weiss;A. Hsu;B. S. Ooi
  24. Mol. Cell Biol. v.7 Differman papillomavirus type 16 DNA-induced maliganant transformation Yasumoto, S.;J. Donigen;J. A. Dipaolo
  25. J. Virol. v.62 Progression of the phenotype of transformedcells after growth stimulation of cells by a human papillomavirus type 16 gene function Noda, T.;H. Yajima;Y. Ito
  26. J. Virol. v.63 The E6 and E7 genes of the human papillomavirus type 16 together are necessary and sufficient for transformation of primary human keratinocytes Munger, K.;W. C. Phelps;V. Bubb;P. M. Howley;K. Schlegel
  27. J. Cell. Eng. v.1 Stable expression of human compliment receptor CR1 gene on rat renal glomerular epithelial cell SGE1 Wang, P. C.;S. Kimura;K. Todokoro;T. Okigaki;Y. Kao;Matsumura, M.
  28. Arti. Organs v.2 Construction of glomerular epithelial cells in vitro for removal of immune complex Wang, P. C.;K. Horisawa;M. Matsumura
  29. Soc. Exp. Biol. Med. The Biology of mesangial cells in glomerulonephritis biology of mesangial cells Veis, J. H.;W. Yamashita;Y. J. Liu;B. S. Ooi
  30. Kidney Int. v.38 Cellular receptors for matrix proteins in normal human kidney and human mesangial cells Cosio, F. G.;D. D. Sedemak;S. Jr. Wahman