Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Purification and Properties of an Inulinase Produced by Arthrobacter sp.

Arthrobacter sp.가 생산하는 Inulinase의 정제 및 성질

  • 임성일 (한국식품개발연구원 생물공학연구본부) ;
  • 이대희 (한국식품개발연구원 생물공학연구본부) ;
  • 홍석산 (한국식품개발연구원 생물공학연구본부) ;
  • 김현규 (한국식품개발연구원 생물공학연구본부) ;
  • 유진영 (한국식품개발연구원 생물공학연구본부)
  • Published : 2000.08.01
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Abstract

The inulinase producing microorganism was isolated from soil and tentatively identified as Arthrobacter protophormiae/ramosus. Inulinase was pruified by ethanol precipitation, DEAE-Sephadex ion exchange chromatography and Sephadex gel filtration chromatography. The molecular weight of the purified enzyme was 34 kDa. The specific activity, yield and purity were 31.5 Unit/mg, 19.5% and 18.5 fold, respectively. Optimal pH and temperature for reaction of the purified inulinase were 8.5 and $55^{\circ}C$, respectively. The enzyme was stable at pH 7.5, below$ 55^{\circ}C$, and the activity was stimulated Mg2+.

Keywords

References

  1. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Bradford, M. M.
  2. Enzyme Micro. Technol. v.22 Synthesis of novel disaccharides by a newly isolated fructosyl transferase from B. subtilis Cheetham, P. S. J.;A. J. Hacking;M. Vlitos
  3. Anal. Chem. v.28 Colorimetric method for determination of sugars and related substances Dubois, M. G.;K. A. Gilles;J. K. Hamilton;P. A. Rebers;F. Smith
  4. CRC Critical Reviews in Food Science and Nutrition v.12 Preparation of highfructose syrup from the tubers of the Jerusalem artichoke(Heliantus tuberosus L.) Fleming, S. E.;J. W. GrootWassink
  5. Agr. Biol. Chem. v.44 Etude de I,inulinase de Candida salmenticensis Van Uden et Buckley Guiraud, J. P.;C. V. Gaudin;P. Galzy
  6. Kor. J. Appl. Microbiol. Biotechnol v.19 Purification and properties of Aspergillus ficuum endoinulinase Han, S. B.;H. S. Ryu;M. W. Rho;T. K. Lee;H. S. Sohn;S. J. Woo;T. B. Uhm
  7. J. Kor. Soc. Food Nutr. v.17 Studies on inulase from Jerusalem artichoke Jhon, D. Y.;M. H. Kim
  8. J. Kor. Agric. Chem. Soc. v.36 Purification and properties of inulin fructotransferase(depolymerizing) from Enterobacter sp.S45 Kang, S. I.;S. I. Kim
  9. Biotechnol. Bioeng. v.33 Stability of immobilized maltotetraose-forming amylase from Pseudomonas stutzeri Kimura, T.;M. Okada
  10. Agric. Biol. Chem. v.53 Immobilization of exo-maltotetrahydrolase and pullulanse Kimura, T.;M. Yoshida;K. Oishi;M. Ogata;T. Nakakuki
  11. Biotechnol. Bioeng. v.32 Continuous production of maltotetraose using immobilized Pseudomonas stutzeri amylase Kimura, T.;M. Okada
  12. J. Kor. Agr. Chem. Soc. v.30 Purification and characterization of intracelluar and extracellular inulase from Kluyveromyces marxianus Kim, S. I.;H. S. Moon
  13. Kor. J. Appl. Microbiol. Biotechnol. v.18 Purification and properties of extracellular inulinase from Bacillus sp. Kim, K. N.;Y. J. Choi
  14. Advances in Biochemical Engineering v.32 Industrial processing and products from the Jerusalem Artichoke Kosaric, N.;A. Wieczorek;G. P. Copsentino;Z. Duvnjak
  15. Kor. J. Appl. Microbiol. Bioeng v.1 Studies on the inulin hydrolyzing enzyme produced by Aspergillus sp.(C-74 strain), Part 1. Some properties of the inulin hydrolyzing enzyme from selected strain Kwon, T. J.;H. K. Chung;H. W. Kang;J. H. Seu
  16. Kor. J. Food & Nutr. v.10 Partial purification and properties of inulinase from garlic(Allium sativum L) Lee, J. S.;S. J. Kwon;S. H. Yi;N. M. Kim;J. Y. Yoo
  17. Kor. J. Appl. Microbiol. Bioeng. v.15 Culture condition for inulase production by Pseudomonas species Lee, T. K.;H. C. Sung;Y. J. Choi;H. C. Yang
  18. Kor. J. Appl. Microbiol. Biotechnol. v.28 Screening and identification of an inulinase producing micro-organism and optimal condition for the enzyme production Lim, S. I.;D. H. Lee;S. S. Hong;J. Y. Yoo
  19. J. Biol. Chem. v.193 Protein measurement with the folin phenol reagent Lowry, O. H.;N. J. Rosebrough;A. L. Farr;R. J. Randall
  20. Kor. Biochem. J. v.10 Purification and characterization of inulase from Khiyveromyces fragilis Nahm, B. H.;S. M. Byun
  21. J. Agr. Chem. Soc. v.52 Culture conditions for inulinase production by Aspergillus Nakamura, T.;S. Maruki;S. Nakatsu;S. Ueda
  22. Nippon Nogeikagaku Kaishi v.51 General properties of extracellular inulase from Penicillium Nakamura, T.;S. Nakatsu
  23. Denpun Kagaku v.36 The production of fructooligosaccharides from inulin or sucrose using inulinase or fructosyltransferase from Aspergillus ficuum Norman, B. E.;B. Hojer-Pedersen
  24. Kor. J. Appl. Microbiol. Biotechnol. v.23 Production of inulin fructotransferase(depolymerizing) by Arthrobacter sp.A-6 Park, J. B.;Y. M. Kwon;Y. J. Choi
  25. Kor. J. Appl. Microbiol. Biotechnol. v.19 Hydrolysis of inulin by endo-and exo-inulinase Park, S. G.;Y. J. Choi
  26. Enzyme Microbiol.Technol v.11 New thermostable α-amylase-like pullulanase from thermophilic Bacillus sp.3183 Saha, B. C.;G. J. Shen;K. C. Srivastava;L. W. LeCureux;J. G. Zeikus
  27. J. Kor. Agr. Chem. Soc. v.28 Aconstitute inulinase from a Bacillus Uhm, T. M.;J. S. Hong;H. S. Shon;M. K. Park;S. M. Byun