Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Effect of Ganoderma lucidum Mycelial Fractions on the Functional Recovery of Primary Cultured Hepatocytes.

영지 균사체 분획이 일차배양 간세포 기능회복에 미치는 영향

  • 박혜선 (서울대학교 약학대학 종합약학연구소) ;
  • 현진원 (서울대학교 의과대학 약리학교실) ;
  • 김하원 (서울시립대학교 생명과학과) ;
  • 심미자 (서울시립대학교 생명과학과) ;
  • 김병각 (서울대학교 약학대학 종합약학연구소)
  • Published : 2000.08.01
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Abstract

The cultured mycelia of Ganoderma lucidum were extracted and the extract was separated into six fractions by organic solvent fractionation. The antihepatotoxic activity of all the fractions was evaluated by measuring activities of glutamic pyruvic transaminase (GPT) and glutamic oxaloacetic transaminase (GOT). Among the fractions tested, the high-polarity fractions such as aqueous and n-butanol fractions significantly reduced activities of GPT and GOT in CCl4- and galactosamine-intoxicated rat primary hepatocytes. When intracellular synthetic activities were measured by pulsing the rate primary cultured hepatocytes with [3H]-uridine and [3H]-leucine, activities of DNA, RNA and protein. When direct toxicities of the fractions were measured against human hepatoma(SK-Hep-1), the non-polarity fractions such as n-butanol and ethyl acetate fractions showed potent direct cytotoxicities even at the concentration of 1 $\mu\textrm{g}$/ml. These data showed that Ganoderma lucidum has hepatoprotective and hepatotoxic recovery principles in its mycelia.

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References

  1. Mol. Cells v.7 Suppressive effects of Ganoderma lucidum on proliferation of peripheral blood mononuclear cells Kim, R. S.;H. W. Kim; B. K. Kim
  2. Int. J. Med Mushrooms v.1 Biomedical triterpenoids of Ganoderma lucidum (Curt.:Fr.) P.Karst.(Aphyllophoromycetideae) Kim, H. W.;B. K. Kim
  3. Agric Biol. Chem. v.52 Bitterness and structure relationship of the triterpenoids from Ganoderma lucidum(Reishi) Nishitoba, T.;H. Sato;S. Sakamura
  4. J. Nat. Prod. v.54 Novel cytotoxic principles of formosan Ganoderma lucidum Lin, C. N.;W. P. Tome;S. J. Won
  5. Chem. Pharm. Bull. v.34 Ganoderic acids, T,S and R, new triterpenoids from the cultured mycelia of Ganoderma lucidum Hirotani, M.;C. Ino;T. Furuya;M. Shiro
  6. Chem. Pharm. Bull. v.37 Ganoderic acid and its derivatives as cholesterol synthesis inhibitors Komoda, Y.;M. Shimizu;Y. Sonoda;Y. Sato
  7. S. Biochim. Biophys. Acta v.986 The aggregation of human platelct induced by ganodermic acid Wang, C. N.;J. C. Chen;M. S. Shiao;C. T. Wang
  8. Biochem. J. v.277 The inhibition of human platelet function by ganodermic acids Wang, C. N.;J. C. Chen;M. S. Shiao;C. T. Wang
  9. Chem. Pharm. Bull. v.34 Angiotensin converting enzyme-inhibitory triterpenes from Ganoderma lucidum Morigiwa, A.;K. Kitabatake;Y. Fujimori;N. Ikekawa
  10. Chem Pharm. Bull v.33 The biologically active constituents of Ganoderma lucidum(Fr.) Karst. histamine release-inhibitory triterpenes Kohda, H.;W. Tokumoto;K. Sakamoto;M. Fujii;Y. Hirai;K. Yamasaki;Y. Komoda;H. Nakamura;S. Ishihara;M. Uchida
  11. Kor. J. Pharmacogn v.24 Studies of constitutents of higher fungi of Korea(LXV III). Antitumor components of the cultured mycelia of Ganoderma lucidum Kim, B. K.;H. Y. Cho;J. S. Kim;H. W. Kim;E. C. Choi
  12. Kogaku and Seibutsu v.23 Medicinal and dietary effects of Mannentake(Reishi). antitumor polysaccharides and bitter terpenoids Mizuno, T.;S. Sakamura
  13. Int. J. Immunopharmacol v.13 Immunomodulator, LZ-8, prevents antibody production in mice Kino, K.;T. Sone;J. Watanabe;A. Yamashita;H. Tsuboi;H. Miyajima;H. Tsunoo
  14. Methods Cell Biol v.13 Preparation of isolated rat liver cells Seglen, P. O.
  15. Culture of Epithelial Cells Isolation and culture of animal and human hepatocytes Guguen-Guillouzo, C.;Freshney, R. I.(ed.)
  16. Cancer Res. v.47 Evaluation of a tetrazolium based semi-automated colormetric assay, assessment of chemosensitivity testing Carmichael, J.;W. G. Degraff;A. F. Gazdar;J. D. Minna;J. B. Mitchell
  17. Planta Medica v.49 Assay method for antihepatotoxic ativity using carbon tetrachloride induced cytotoxicity in primary cultured hepatocytes Kiso, Y.;M. Tohkin;H. Hikino
  18. Eur. J. Drug Metab. Pharmacokine v.21 Antioxidant action of a new flavonic derivative in acute carbon tetrachloride intoxication Paduraru, I.;A. Saramet;G. Danila;M. Nichifor;L. Jerca;A. Iacobovici;D. Ungureanu;M. Filip
  19. Drug Merab Dispos v.16 Effect of galactosamine-induced hepatic UDP-glucuronic acid depletion on acetaminophen elimination in rats. Dispositional differences between hepatically and extrahepatically formed glucuronides of acetaminophen and other chemicals Gregus, Z.;C. Madhu;D. Goon;C. D. Klaassen