한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제28권2호
  • /
  • Pages.87-91
  • /
  • 2000
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Terrein의 etoposide에 의해 유도된 apoptosis 저해효과

Anti-apoptotic Effects of Terrein on Etoposide-induced Apoptosis of U937 Human Leukemia Cells

  • 이충환 (생명공학연구소 면역제어물질 연구실) ;
  • 이호재 (생명공학연구소 면역제어물질 연구실) ;
  • 김진희 (생명공학연구소 면역제어물질 연구실) ;
  • 김현아 (생명공학연구소 면역제어물질 연구실) ;
  • 고영희
  • 발행 : 2000.04.01
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초록

미생물로부터 U937 세포주의 etoposide에 유발된 apoptosis 저해물질을 탐색한 결과 곰팡이 F80834 균주를 선발하였다. 균주 배양액으로부터 저해물질을 분리한 후 UV, EIMS, 1H-NMR, 13C-NMR, DEPT 등의 기기분석을 실시한 결과 terrein으로 동정되었다. 이 물질은 $IC_{50}$ $20\mu\textrm{g}/ml$의 농도로 U937 세포주의 etoposide에 의한 caspase 3 유도를 저해하였다. Etoposide에 의한 세포의 사멸도 IC50 $10\mu\textrm{g}/ml$ 농도로 저해하였으며, 동일한 농도 조건에서 단독 처리시 세포 독성을 나타내지 않았다.

In the course of screening for the substances inhibiting apoptosis ofU937 human leukemia cell induced by etoposide, a fungal strain F80834 producing a high level of inhibitor was selected. The inhibitory substance was purified and identified as terrein by spectroscopic methods of UV, EI-MS, IH-NMR, 13C-NMR and DEPT. Terrein showed inhibitory activity of caspase 3, a major protease of apoptosis cascade, with an $IC_{50}$ value of $20\mu\textrm{g}/ml$ after 7 hrs of treatment. It also showed protective effect against cell death with an $IC_{50}$ value of $10\mu\textrm{g}/ml$ on U937 cells induced by etoposide after 24 hrs of treatment, but did not show any cytotoxicity at the same condition without etoposide.

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