생명과학회지 (Journal of Life Science)

  • 제10권6호
  • /
  • Pages.610-616
  • /
  • 2000
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

출아효모에서 S기 checkpoint에 관여하는 DPB11에 관한 연구

DPB11 is a component of the S-phase checkpoint in Saccharomyces cerevisiae

  • 임선희 (동아대학교 자연과학대학 생물학과) ;
  • 박정은 (동아대학교 자연과학대학 생물학과) ;
  • 김중현 (동아대학교 자연과학대학 생물학과) ;
  • 오규선 (동아대학교 자연과학대학 생물학과) ;
  • 선우양일 (동아대학교 자연과학대학 생물학과) ;
  • 정정남 (동아대학교 자연과학대학 생물학과)
  • 발행 : 2000.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The DPB11 gene, which genetically interacts with DNA polymerase II ($\varepsilon$) encoded by POL2 and DPB2, is required for DNA replication and the S phase checkpoint in Saccharomyces cerevusiae. The transcripts of DPB11 did not fluctuated as evidently as DPB2 and POL2 genes during cell cycle. To identify the physical interaction between Dpb2 and Dpb11, we examined the interaction by two-hybrid system. The interaction between Dpb2 and Dpb11 was detected in a two-hybrid assay. These results suggest that the amount of the Dpb2-Dpb11 complex may peak at the G1/S boundary. Therefore, we strongly suggest the involvement of the Dpb2-Dpb11 complex in a progression of DNA replication and S-phase checkpoint.

키워드

참고문헌

  1. EMBO J. v.11 DNA polymerase II, the probable homolog of mammalian DNA polymerase ε, replicates chromosomal DNA in the yeast Saccharomyces cerevisiae Araki, H.;P. A. Ropp;A. L. Johnson;L. H. Johnston;A. Morrison;A. Sugino
  2. Nucleic Acids Res. v.19 Cloning DPB3, the gene encoding the third subunit of DNA polymerase II of Saccharomyces cerevisiae Araki, H.;R. K. Hamatake;A. Morrison;A. L. Johnson;L. H. Johnston;A. Sugino
  3. Proc. Natl. Acad. Sci. USA v.88 DPB2, the gene encoding DNA polymerase II subunit B, is required for chromosome replication in Saccharomyces cerevisiae Araki, H.;R. K. Hamatake;L. H. Johnston;A. Sugino
  4. Proc. Nati. Acad. Sci. USA v.92 Dpb11, which interacts with DNA polymerase II (ε) in Saccharomyces cerevisiae, has a dual role in S phase progression and at a cell cycle checkpoint Araki, H.;S.-H. Leem;A. Phongdara;A. Sugino
  5. Methods Enzymol. v.283 Gene identification using the yeast two hybrid system Bai, C.;S. J. Elledge
  6. J. Biol. Chem. v.265 Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae Hamatake, K. R.;H. Hasegawa;A. B. Clark;K. Bebenek;T. A. Kunkel;A. Sugino
  7. Science v.246 Checkpoints: controls that ensure the order of cell cycle events Hartwell, L.;T. weinert
  8. Mol. Cell. Biol. v.13 A multicopy suppressor gene of the Saccharomyces cerevisiae G1 cell cycle mutaiton, dbf4, encodes a protein kinase and is identified as CDC5 Kitada, K.;A. L. Johnson;L. H. Johnston;A. Sugino
  9. Cell v.80 A third essentail DNA polymerase in S. cerevisiae Morrison, A. H.;H. Araki, B. Clark;R. K. Hamatake;A. Sugino
  10. Current Opin. Genet. Dev. v.5 The genetics of cell cycle checkpoints Murry, A.
  11. Cell v.80 DNA polymerase ε links the DNA replication machinery to the S phase checkpoint Navas, T. A.;Z. Zhou;S. J. Elledge
  12. Nature v.247 Replication of yeast chromosomal DNA Newlon, C. S.;T. D. Petes;L. M. Hereford;W. L. Fangman
  13. Nucleic Acids Res. v.22 The RFC2 gene encoding a subunit of replication factor C of Saccharomyces cerevisiae Noskov, V.;S. Maki;Y. Kawasaki;S.-H. Leem;B.-I. Ono;H. Araki;Y. Pavlov;A. Sugino
  14. Cell v.79 Ordering S phase and M phase in the cell cycle Nurse, P.
  15. Genes Dev. v.11 Damage and replication checkpoint control in fission yeast is ensured by interactions of Crb2, a protein with BRCT motif, with Cut5 and Chk1 Saka, Y.;F. Esashi;T. Matsusaka;S. Mochida;M. Yanagida
  16. EMBO J. v.13 Fission yeast cut5 links nuclear chromatin and M phase regulator in the replication checkpoint control Saka, Y.;P. Fantes;T. Sutani;C. McInerny;J. Creanor;M. Yanagida
  17. Cell v.74 Fission yeast cut5+, required for S phase onset and M phase restraint, is identical to the radiation-damage repair gene rad4+ Saka, Y.;M Yanagida
  18. Molecular cloning: a laboratory manual(2nd ed) Sambrook, J.;E. F. Fritsch;T. Maniatis
  19. Methods in yeast genetics Sherman, F.;G. R. Fink;J. B. Hicks
  20. Science v.274 Cell cycle control of DNA replication Stillman, B
  21. Trends Biochem. Sci. Yeast DNA polymarase and their role at the replication fork Sugino, A.
  22. EMBO J. v.5 periodic transcription as a means of regulating gene expression during the cell cycle : contrasting modes of expression of DNA ligase genes in budding and fission yeast White, J. H. M.;D. G. Barker;P. Nurse;L. H. Johnston