Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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DPB11 is a component of the S-phase checkpoint in Saccharomyces cerevisiae

출아효모에서 S기 checkpoint에 관여하는 DPB11에 관한 연구

  • 임선희 (동아대학교 자연과학대학 생물학과) ;
  • 박정은 (동아대학교 자연과학대학 생물학과) ;
  • 김중현 (동아대학교 자연과학대학 생물학과) ;
  • 오규선 (동아대학교 자연과학대학 생물학과) ;
  • 선우양일 (동아대학교 자연과학대학 생물학과) ;
  • 정정남 (동아대학교 자연과학대학 생물학과)
  • Published : 2000.12.01
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Abstract

The DPB11 gene, which genetically interacts with DNA polymerase II ($\varepsilon$) encoded by POL2 and DPB2, is required for DNA replication and the S phase checkpoint in Saccharomyces cerevusiae. The transcripts of DPB11 did not fluctuated as evidently as DPB2 and POL2 genes during cell cycle. To identify the physical interaction between Dpb2 and Dpb11, we examined the interaction by two-hybrid system. The interaction between Dpb2 and Dpb11 was detected in a two-hybrid assay. These results suggest that the amount of the Dpb2-Dpb11 complex may peak at the G1/S boundary. Therefore, we strongly suggest the involvement of the Dpb2-Dpb11 complex in a progression of DNA replication and S-phase checkpoint.

Keywords

References

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