생명과학회지 (Journal of Life Science)

  • 제10권5호
  • /
  • Pages.533-541
  • /
  • 2000
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

두툽상어 연골 조직에서 생리 활성 유전자들의 cDNA 클로닝

Cloning of various bioreactive genes from cartilage tissues of Scyliorhinus torazame

  • 김지태 (부산대학교 자연과학대학 분자생물학과) ;
  • 김명순 (부산대학교 자연과학대학 분자생물학과) ;
  • 장은령 (부산대학교 자연과학대학 분자생물학과) ;
  • 김영진 (부산대학교 자연과학대학 분자생물학과) ;
  • 김규원 (서울대학교 약학대학 약학과)
  • 발행 : 2000.10.01
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초록

Compared to mammal including human, many bioreactive genes that regulate various biological events has not been cloned and characterized yet in fishes, especially shark, Scyliorhinus torazame. In orther to isolate genes that regulate physiological processes in cartilaginors fishes, we performed reverse transcription-polymerase chain reaction (RT-PCR) using the RNA of cartilage tissues of Scyliofhinus torazame. The cloned partial genes were 86%, 80%, 73%, 84%, 75%, 79% identical to $\alpha$- actin, 90-kDa heat-shock protein, methyle-neterahydrofolate dehydrogenase-methenyltertrahydrofolate cyclohudrolase-formyltetrahydrofolate synthetase, ubiquitin, glutamine synthetase and connective tissue growth factor genes of human, respectively. They also have similar nucleotide sequence homologues with those of another species. These partial bioreactive genes elucidated in this study may support to studies of phylogenetic analysis based on evolutionary relationships between shark and other species.

키워드

참고문헌

  1. J. Biol. Chem. v.275 Tumor necrosis factor alpha suppresses the induction of connective tissue growth factor by transforming growth factor-beta in normal and scleroderma fibroblasts. Abraham,D. J.;Shiwen, X.;Black, C. M.;Sa, S.;Xu,Y.;Leask, A.
  2. Biochem. Biophys. Res. Commun. v.13 A simple method for the quantitative isolation of undegraded high molecular weight ribonucleic acid. Barlow, J. J.;Mathias,A. P.;Williamson, R.;Gammack, D. B.
  3. Mol. Cell. Biol. v.9 hsp82 is an essential protein that in required in higher concentrations for growth of cells at higher temperatures. Borkovich,K.A.;Farrelly, F. W.;Finkelstein,D. B.;Taulien, J.;Lindquist, S.
  4. Biochem. Biophys. Acta. v.1477 Stucture-function relationships of glutamine synethetases. Eisenberg, D.;Gill, H. S.;Pfuegl,G. M.;Rotstein, S. H.
  5. Annu. Rev. Cell Biol. v.7 Ubiquitination. Finley, D.;Chau, V.
  6. Trends Cell Biol. v.10 Ubiquitin and its kin: how close are the family ties? Jentsch, S.;Pyrowolakis, G.
  7. Korean Association of Cancer Prevention v.5 Anti-angiogenic Effect of Tissue Inhibitor of Metalloproteinase-3(TIMP-3) from Shark, Scyliorhinus torazama. Kim, J. T.;Jeong, J.-W.;Sohn, T.-K.;Kim, K.-W.
  8. Biochem. Biophys. Acta. Cloning and characterization of tissue inhibitor of metalloproteinase-3 (TIMP-3) from shark, Scyliorhinus torazame. Kim, J. T.;Kim, M.-S.;Bae,M.-K.;Song, H.S.;Ahn, M.-Y.;Kim, Y.-J.;Lee,S.-J.;Kim,K.-W.
  9. Korean Association of Cancer Prevention v.4 Cloning of membrane type-3 matrixmetalloproteinase(MT-3MMP) gene from Scyliorhinus torazame. Kim, M.-S.;Bae,M.-K.;Kim,J.-T.;Song,H. S.;Ahn, M.-Y.;Kim,J. W.;Lim, W. K.;Kim, K.-W.;Kim,Y.-J.
  10. Cloning and Characterization of membrane type-3 matix metalloproteinase (MT3-MMP) from Scyliorhinus torazame. Kim, M.-S.;Bae,M.-K.;Kim, J.-T.;Jeong,J.-W.;song, H.-S.;Ahn,M.-Y.;Kim,J. W.;Lim, W. K.;Kim, K.-M.;Kim,Y.-J.
  11. Cell v.45 Intracellular localization of messenger RNAs for cytoskelstal proteins. Lawrence, J. B.;R. H. Singer
  12. Science v.221 Shark cartilage contains inhibitors of tumor angiogenesis. Lee, A.;Langer R.
  13. J. Clin. Invest. v.103 Alphacardiac actin a novel disease gene in familial hypertrophic cardiomyopathy. Mogensen, J.;Klausen,I. C.;Pedersen, A, K.;Egeblad, H.;Bross, P.;Kruse,T. A.;Gregersen, N.;Hansen, P. S.;Baandrup, U.;Borglum, A. D.
  14. Mol. Genet. Metab. v.71 Connective Tissue Growth Factor: What's in a Name? Moussad, E. E.;Brigstock D. R.
  15. Nat. Genet. v.23 Mutations in the skeletal muscle alpha-actin gene in patients with actin myopathy and nemaline myopathy. Nowak, K. J.;Wattanairichaigoon, D.;Goebel, H. H.;Wilce, M.;Pelin, K.;Donner, K.;Jacob, R. L.;Hubner, C.;Oexle, K.;Anderson, J. R.;Verity, C. M.;North,K. N.;Iannaccone, S. T.;Muller,C. R.;Nurnberg,P.;Muntoni, F.;Wewry,C.;Beggs, A. H.;Laing, N. G.
  16. Cancer Lett. v.51 A novel angiogenic inhibitor derived rrom Japanese shark cartilage (I). Extraction and estimation of inhibition activities toward tumor and embryonic angiogenesis Oikawa, T.;Ashino-Fuse, H.;Shimamura, M.;Koide, U.;Iwaguchi, T.
  17. Science v.280 Antin mutations in dilated cardiomypathy, a heritable form of heart failure. Olsen, T. M.;Michels, V. V.;Thibodeau, S. N.;Tai,Y. S.;Keating, M. T.
  18. Am. J. Hum. Genet. v.44 Chromosomal localization of the gene for the human trifunctional enzyme, methylenetetrahydrofolate dehyrogenase-methenyltetrahydrofolate cyclohydrolase-formyltetrahydrofolate synthetase. Rozen, R.;Barton, D.;Du, J.;Hum, D. W.;Mackenzie,R. E.;Francke, U.
  19. J. Biochem. v.124 Inhibition of endogenous expression of connective tissue growth factor by its antisense oligonucleotide and antisense RNA suppresses proliferation and migration of vascular endothelial cells. Shimo, T.;Nakanishi,T.;Kimura, Y.;Nishida, T.;Ishizeki, K.;Matsumura, T.;Takigawa, M.
  20. Cancer Res. v.56 Molecular analysis of the FHIT gene at 3p14.2 in lung cancer cell lines. Yanagisawa, K.;Kondo, M.;Osada, H.;Uchida, K.;Takagi, K.;Masuda,A.;Takaashi,T.;Takahashi, T.
  21. Biochem. Biophys. Res. Commun. v.259 Direct comparison of GAPDH, β-actin, cyclophilin, and 28S rRNA as internal standards for quantifying RNA levels under hypoxia. Zhong, H.;Simon, J. W.
  22. Curr. Opin. Struct. Biol. v.10 Structure and in vivo function of Hsp90 Pearl, L. H.;Prodromou, C.