Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Silk Fibron on Oxidative Stress and Membrane Fluidity in Brain of SD Rats

뇌조직의 산화적 스트레스 및 세포막 유동성에 미치는 실크 피브로인의 영향

  • 최진호 (부경대학교 식품생명공학부 생화학교실) ;
  • 김대익 (부경대학교 식품생명공학부 생화학교실) ;
  • 박수현 (부경대학교 식품생명공학부 생화학교실) ;
  • 김정민 (부경대학교 식품생명공학부 생화학교실) ;
  • 이종수 (부경대학교 식품생명공학부 생화학교실) ;
  • 이광길 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 여주홍 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 이용우 (농촌진흥청 농업과학기술원 잠사곤충부)
  • Published : 2000.10.01
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Abstract

This study was designed to investigate the effects of silk fibroin powder (SFP : Mw 500) on oxidative stress and membrane fluidity in brain membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (SFP-2.5 and SFP-5.0 groups) added 2.5 and 5.0 g/kg BW/day for 6 weeks. Cholesterol level was significantly decreased about 8.0% in brain microsomes of SFP-5.0 group only compared with control group. Membrane fluidities were significantly increased (12.9% and 15.2%, respectively) in brain microsomes of SFP-2.5 and SFP-5.0 groups, but significant difference between in brain mitochondria of these two groups could be not obtained. Basal oxygen radicals (BOR) in brain mitochondria and microsomes were significantly ingibited (10.4%, and 24.0%, 7.9% and 14.9%, respectively) by SFP-2.5 and SFP-5.0 groups compared with control group. Induced oxygen radicals (IOR) in brain mitochondria and microsomes were significantly inhibited (11.8% and 14.1%, respectively) by SFP-5.0 groups compared with control group compared with control group. Lipid peroxide (LPO) levels were dose-dependently decreased (12.9% and 21.9%, 13.2% and 22.5%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-5.0 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (15.7% and 17.1%, 16.7% and 15.7%, respectively) in brain mitochondria and microsomes of SFP-2.5 and SFP-2.5 and SFP-5.0 groups compared with control group. These results suggest that administration of SFP may play an effective role in a attenuating a oxidative stress and increasing a membrane fluidity in brain membranes.

Keywords

References

  1. J. Sericultural Science of Japan v.60 no.5 The digestion of silk fibroin by rat. Chin, K.;K. Iura;R. Aizawa;K. Hirabayashi
  2. Korena J. Nutr. v.31 no.7 Effects of silkworm powder on blood glucose and lipid levels in NIDDM(type-II)patients. Cho, M. R.;R. W. Choue;S. H. Chung;J. W. Ryu
  3. Korena J. Nutr. v.31 no.7 A study of folk remedies in type-II diabetic patients. Cho, M. R.;R. W. Choue
  4. Age v.13 Unsuitability of TBA test as a lipid peroxidation marker due to prostaglandin synthesis in the aging kidney. Choi, J. H.;B. P. Yu
  5. Kor. J. Biochem. v.23 no.1 Lipid peroxidation, aging and food restriction. Choi, J. H.
  6. Free Rad. Biol & Med. v.18 no.2 Brain synaptosomal aging : Free radicals and membrane fluidity. Choi, J. H.;B. P. Yu
  7. Age v.19 Analysis of lipid composition and hydroxyl radicals in brain membranes of senescence-accelerated mice. Choi, J. H.;D. I. Kim; Y. S. Moon;H. Y. Chung;B. P. Yu
  8. Korean J. Seric. Sci. v.41 no.3 Effects of mulbery leaf extract on oxygen radicals and their scavenger enzymes in serum of SD rats. Choi, J. H.;D. I. Kim;S. H. Park;D. W. Kim;J. S. Lee;K. S. Ryu;W. C. Lee
  9. Korean J. Seric. Sci. v.41 no.3 Effects of silkworm powder on oxygen radicals and their scavenger enzymes in serum of SD rats. Choi, J. H.;D. I. Kim;S. H. Park;D. W. Kim;J. S. Lee;H. S. Lee;K. S. Ryu
  10. Korean J. Seric. Sci. v.41 no.3 Effects of silk fibroin powder on oxygen radicals and their scavenger enzymes in serum of SD rsts. Choi, J. H.;D. I. Kim;S. H. Park;Park, S. H.;D. W. Kim;J. S. Lee;Y. W. Lee
  11. Functional anti-diabetic drink. Choi, J. H.;Presidant of RDA
  12. Functional anti-diabetic drink. Choi, J. H.;Presidant of RDA
  13. Korean J. Seric. Sci. v.42 Effects of Effects of silk fibroin on oxidative stress and membrane fluidity in brain memnbranes of SD rats. Choi, J. H.;D. I. Kim;S. H. Park;J. M. Kim;H. S. Lee;K. S. Ryu
  14. Bull. K. H. Pharma. Sci. v.24 Blood glucose lowering effect of silkworm. Choug. S. H.;J. H. Yu;E. J. Kim;K. S. Ryu
  15. Korean J. Seric. Sci. v.39 no.1 Effect of silkworm extract on intestinal α-glycosidase activity in mice administered with a high carbohydrate-containing diet. Choig, S. H.;M. S. Kim;K. S. Ryu
  16. J. Gerontol. v.11 Aging : a theory based on free radical and radiation chemistry. Harman, D.
  17. Proc. Natl. Acad. Sci. v.12 Lipid fluidity markedly markedly modulates the binding of serotonin to mouse brain membranes. Heron, D. S.;M. Shinitzky;M. Hershkowiz;D. Samuel
  18. Bio Industry v.6 no.10 Application of water soluble silk. Hirabayashi, K.
  19. J. of the Japanese Sec. for Food Sci. and Technology v.45 no.11 Foaming properties of fibroin solution prepared from silk yarn and utilization of Foam for making aponge cake. Hirao, K.;Y. Kimura;K. Igarashi
  20. New Food Industy v.33 no.11 Utilization of silk fibroin as food ingredient. Hirabayashi, K.;K. Chin;T. Sebata
  21. J. Korean Soc. Food Nutr. v.24 no.4 The effect of dry edible leaves feeding on serum lipids of hyperchole-sterolemic rats. Kang, J. O;K. S. Kim
  22. J. Korean Soc. Food Sci. Nutr.I v.27 no.6 Antihyperlipidemic effects of methanol extracts from mulberry leaves in cholesterol-induced hyperlipidemia rats. Kim, S. Y.;W. C. Lee;H. B. Kim;A. J. Kim;S. K. Kim
  23. Biochem. Biophys. Res. Commun. v.145 Anti-lipoperoxidation action of food restriction. Laganiere, S.;B. P. Yu
  24. Biochem. Biophys. Res. Commun. v.163 no.2 Perturbations in cerebral oxygen radical formation and membrane order following vitamin E deficiency. Lebel C. P.;I. N. Odunze Jr;S. C. Bondy
  25. Korean J. Seric. Sci. v.40 no.1 Effect of several sericultural products on blood glucose lowering for allixan-induced hyper-glycemic mice. Lee, H. S.;K. S. Chung;S. Y. Kim;K. S. Ryu;W. C. Lee
  26. Methods Enzymol. v.1986 Determination of carbonyl content in oxidatively modified proteins. Levine, R. L.;D. Garland;C. N. Oliver;A. Amici;I. Climent;A. G. Lenz;B. Ahn;S. Shaltiel;E. R. Stadtman
  27. J. Biol. Chem. v.193 Protein measurement with the Folin-Phenol reagent. Lowry, O. H.;N. J. Roseborough;L. A. Farr;R. J. Randall
  28. J. of Sericultural Science of Japan v.63 Production of silk powder and properties. Lu, X.;D. Akiyama, K;Hirabayashi
  29. J. Japan Soc. Nutr. Food Sci. v.41 no.1 Effect of disacchariase inhibitor, AO-128, on postprandial hyperglycemia in rats. Osaka, H.;n. Miki;H. Ikeda;T. Matsuo
  30. Agric. Chem. Biotech. v.41 no.2 Cytotoxic activity of Bombyx mori and Moru alba derived materials against human tumor cell lines. Park, I. K;J. O. Lee;H. S. Lee;K. Y. Seol;Y. J. Ahn
  31. J. Lipid Res. v.14 Determination of cholesterol using ο -phthalaldehyde. Rudel, L. L;M. D. Morris
  32. Korean J. Seric. Sci. v.39 no.1 An activity of lowering blood-glucose levels according to prelarative conditions of silkworm powder. Ryu, K. S.;H. S. Lee;S. H. Chung;P. D. Kang
  33. J. New Remedies & Clinics v.47 no.1 Significance of peptidoglycan in patients with chronic liver diseases. Shiomi, S.;D. Habu;T. Takeda;S. Nishiguchi;T. Kuroki;T. Tanaka;K. Tsuchida;S. Yamagami
  34. Principles and procedures of statistics. Steel, R. G. D.;J. H. Torrie
  35. J. Japan Soc. Food and Nutrition v.42 Importance of sulfur-containing amino acids in cholesterol meta-bolism. Sugiyama, K.
  36. Chemistry and Physics of Lipids v.45 Lipid peroxides and human diseases. Yagi, K.
  37. J. Biol. Chem. v.261 Extraction and identification of antioxidant components from the leaves of mulberry (Morus alba L.). Yen, G. C.;S. C. Wu;P. D. Duh
  38. Free Rad Biol. Med. v.21 Aging and oxidative stress : Modulation by dietary restriction. Yu, B. P
  39. Ann. N. Y. Acad. Sci. v.786 Critical evaluation of free radical theory of aging : A proposal of oxidative stress hypothesis. Yu, B. P.;R. Yang