Detection of Enterovituses from Surface Water by Combined Cell Culture-PCR

지표수로부터 세포배양-연계 PCR법에 의한 장바이러스의 검출

Enterovirues may cause gastrointestinal symptoms, cold, and fever, mainly in young children. They are also recognized as important agents in acute infections of the central nervous system such as meningitis and encephalitis, and in subacute and chronic infections of the cardiovascular system such as pericarditis, myocarditis and cardiomyopathy. They also can lead to postviral fatigue syndrome. For the detection of enteroviruses from the environmental samples, the combined cell culture-polymerase chain reaction (CC-PCR) technique was employed. In contrast to EPA standard method which mainly depends on the cell culture, it involved the use of cell culture, followed by PCR to improve the sensitivity and the accuracy of the test. According to the results of survey, from 1999 to 2000, for the presence of enteroviruses in the surface water samples from Nak-dong river, four out of twelve samples were positive for viruses. The titer of viruses in the surface water was ranged from 25 to 250 MPN. All of the viruses isolated were poliovirus type I with 98% nucleotide sequence homology. The result also clearly suggests the seasonal difference in the distribution of the waterborne enteroviruses in surface water because most of the viruses were mainly detected from the summer through the early autumn.