생명과학회지 (Journal of Life Science)

  • 제10권4호
  • /
  • Pages.380-387
  • /
  • 2000
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

비브리오 균속이 생산하는 식중독 유발 Toxin 유전자의 검출

Detection of Food Poisoning Toxin Genes Produced by Vibrio sp.

  • 류병호 (경성대학교 응용공학부 식품공학과) ;
  • 김민정 (경성대학교 응용공학부 식품공학과) ;
  • 조경자 (경성대학교 생활과학부 생활경영학과)
  • 발행 : 2000.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Recently Vibrio sp. are the most frequently isolated microorganism, which causing food poisoning. We investigated the detection of toxin genes and effect of chitosan to toxin genes with PCR. Thirty strains of Vibrio sp. were isolated from sea water and sea products through biological and biochemical tests. Out of 30 strains, 8 were identified as V. parahemolyticus, 7 as V. mimcus, 6 as V. damsela, 5 as V. vulnificus, 4 as V. alginoyticus. In detection of ctx, tdh, and t고 as food poisoning-causing toxin genes, ctx from 7 strains, trh from 4 strains and tdh from 6 strains were detected. Among toxin genes detected strains, we selected V. vulnificus-2 V. damsela-1 and V. parahemolyticus-7. As adding chitosan solution to PCR product of 3 strains, the amplified DNA bands were not detected over 450$\mu\textrm{g}$/$m\ell$ concentrantion of chitosan. Over the result, chitosan is thought to influence the detection of toxin gene.

키워드

참고문헌

  1. FEMS Microbiol. Lett. v.79 Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus. Arita, M.;T. Homda;T. Miwatani;T. Takeda;T. Takao;Y. Shimonishi
  2. Current protocols in molecular biology. Auaubel, F. M.;R. Brent;R. E. Kingston;D. D. Moore;J. G. Seidman;J. A.Smith;K. Struhl
  3. Soc-Appl-Bacteriol-Symp-Ser v.27 Kits for the detection of some bacterial food poisoning toxins: problems, pitfalls and benefits. Brett, M. M.
  4. Infect. Immun. v.34 Production of cholera-like enterotoxin by a Vibrio cholerae non-O1 stain isolated from the enviroment. Craig, J. P.;K. Yamamoto;Y. Takeda;T. Miwatani
  5. Food poisoning 1991-1995 v.7 no.2 Information of Infectious Diseases.
  6. Enteric Gram-negative microorganism. Jawetz, E.;J. L. Melnick;E. A. Adelberg
  7. Infectious diseases. Jeung, H.-Y.;J.-H. Jeun
  8. J. Infect. Dis. v.150 Resistance of Vibrio vulnificus to serum bactericidal and opsonizing factors: relation to virulence in suckling mice and humans. Johnson, J. E.;F. M. Calia;D. M. Musher;A. Goree
  9. J. Bacteriol. v.170 Staphylococ cal enterotoxin B gene is associated with a discrete genetic element. Johnson, M. B. Jr.;S. A. Khan
  10. J. Clin. Microbiol. v.29 Detection of genes for enterotoxins.exfoliative toxins, and toxic shock syndrome toxi 1 in Staphylococcus aureus by the polymerase chain reaction. Johnson, W. M.;S. D. Tyler;E. P. Ewan;F. E. Ashton;D. R. Pollard;K. R. Rozee
  11. J. Kor. Soc. Microbiol. v.25 no.2 Identification of Vibrio species in Hek San Do and Hong Do, Korea. Ju, J.-W.
  12. J. Kor. Soc. Microbiol. v.25 no.4 Isolation of pathogenic Vibrio species in UI Rug Do, Korea. Ju, J.-W.;M.-H. Lee;I. Kim
  13. J. Gen. Microbiol. v.133 Purification and characterization of an elastolytic protease of Vibrio vulnificus. Kothary, M. H.;A. S. Kreger
  14. Infect. Immun. v.33 Dectection of extracelluar toxin(s) produced by Vibrio vulnificus. Kreger, A.;D. Lockwood
  15. Infect. Immun. v.55 Activation of the plasma kallikrein-kinin system by Vibrio vulnificus protease. Miyoshi, N.;S. Miyoshi;K. Sugiyama;Y. Suzuki;H. Furuta;S. Shinoda
  16. Infect Inmmun. v.49 Detection of the thermostable direct hemolysin gene and relared DNA sequences in Vibrio parahemolyticus and other Vibrio species by the DNA colony hybridoization test. Nishinushi,M.:M. Ishibashi;Y. Takeda;J. B. Kaper
  17. FEMS Microbiol. Lett. v.67 Comparative analysis of the hemolysin genes of Vibrio cholerae non-O1, V. mimicus and V. hollisae that are similar to the tdh gene of V. parahemolyticus. Nishibuchi, M.;V. Khaeomaneeian;T. Honda;J. B. Kaper;T. Miwatani
  18. J. Clin. Microbiol. v.35 Analysis of the thermostable direct hemoysin (tdh) gene and the tdh-related hemolysin(trhl) genes in the urease-positive strains of Virio parahemolyticus isolated on the West Coast of the United States. Okuda, J. M;S. L. Ishibashi;J. M. abott;M. Nishuchi
  19. Microbiol. Immunol. v.32 Development of a highly sensitive bead-ELISA to detect bacterial protein toxins. Oku, Y.;Y. Ueasaka;T. Hirayama;Y. Takeda
  20. Developing methodology. Detection and quantitation of foodborn pathogens and their toxins Gram-negative bacterial pathogens. 317, Food-borne microorganism and their toxins. Pierson, M. M.;D. Stern
  21. Developments in Food Microbiology 2. Vibrio species as causative agents of food-borne infection. Sakazaki, R.;T. Shimada;Robison RK(ed.)
  22. A Laboratory Manual. Molecular cloning. Sambrook, J.;E. F. Fritsch;T. Maniatis
  23. J. Clin. Microbiol. v.29 no.11 Polymerase chain reaction for detection of cholera enterotoxin operon of Vibrio cholerae. Shirai, H.:M. Nishibuchi;T. Ramamurthy;S. K. Bhatacharaya;S. C. Pal;Y. Takeda
  24. J. Mol. Biol. v.98 Detection of specific sequences among DNA fragments separated by gel electrophoresis. Southern, E. M.
  25. Nucleic Acids. Res. v.18 DNA polymorphisms amplified by arbitrary primers are useful as genetic markers Williams, J. G.;A. R. Kubelik;K. J. Livak;J. A. Rafalski;S. V. Tingey
  26. Infect. Immun. v.58 Phenotypic evaluation of a capsular transposon mutants of Vibrio vulnificus. Wright, A. C.;L. M.Simpson;J. D. Oliver;J. G. Morris