Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Detection of Food Poisoning Toxin Genes Produced by Vibrio sp.

비브리오 균속이 생산하는 식중독 유발 Toxin 유전자의 검출

  • 류병호 (경성대학교 응용공학부 식품공학과) ;
  • 김민정 (경성대학교 응용공학부 식품공학과) ;
  • 조경자 (경성대학교 생활과학부 생활경영학과)
  • Published : 2000.08.01
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Abstract

Recently Vibrio sp. are the most frequently isolated microorganism, which causing food poisoning. We investigated the detection of toxin genes and effect of chitosan to toxin genes with PCR. Thirty strains of Vibrio sp. were isolated from sea water and sea products through biological and biochemical tests. Out of 30 strains, 8 were identified as V. parahemolyticus, 7 as V. mimcus, 6 as V. damsela, 5 as V. vulnificus, 4 as V. alginoyticus. In detection of ctx, tdh, and t고 as food poisoning-causing toxin genes, ctx from 7 strains, trh from 4 strains and tdh from 6 strains were detected. Among toxin genes detected strains, we selected V. vulnificus-2 V. damsela-1 and V. parahemolyticus-7. As adding chitosan solution to PCR product of 3 strains, the amplified DNA bands were not detected over 450$\mu\textrm{g}$/$m\ell$ concentrantion of chitosan. Over the result, chitosan is thought to influence the detection of toxin gene.

Keywords

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