손바닥선인장(제주도 기념물 35호) 추출물이 면역계세포의 활성화에 미치는 영향

Effects of Opuntia ficus-indica extract on immune cell activation

  • 문창종 (제주대학교 농과대학 수의학과) ;
  • 김승준 (제주대학교 농과대학 수의학과) ;
  • 안미정 (제주대학교 농과대학 수의학과) ;
  • 이선주 (생명과학연구소, 자연과학대학 화학과) ;
  • 정규식 (한국과학기술원 생명공학연구소) ;
  • 박상준 (한국과학기술원 생명공학연구소) ;
  • 윤도영 (한국과학기술원 생명공학연구소) ;
  • 최용경 (한국과학기술원 생명공학연구소) ;
  • 신태균 (제주대학교 농과대학 수의학과, 생명과학연구소)
  • 발행 : 2000.08.01

초록

Opuntia ficus-indca(Op) extract has been claimed to have several therapeutic properties in oriental medicine including anti-inflammatory and anti-rheumatoid arthritis effects. Little is known of its effect on the activation of immune cells such as T cells and macrophages. To evaluate the functional effect of Op extract on immune cells, we examined whether Op extract stimulates the proliferation of T cells and the secretion of cytokines including IL-1 beta, IL-6 and tumor necrosis factor-alpha in THP-1 cell lines by RT-PCR. Op extract significantly enhanced the proliferation of T cell clone(D10S). Transcription of cytokines including IL-1 beta, IL-6, and TNF-alpha peaked 6 hrs after exposure to Op extract(100g/ml) in the THP-1 cell line and declined and declined thereafter. In an experiment to test the dose dependency of transcription of cytokines, transcription increased at a dose of 10 g/ml and the maximum expression was obtained at 100 g/ml, 6 hrs after exposure to Op extract. These findings suggest that Op extract is a potent stimulant of immune cells including T cells and macrophages, which acts by stimulating T cell proliferation and upregulating cytokines. These phenomena imply that some edible plants may be beneficial to living animals through the activation of immune functions.

키워드

참고문헌

  1. Arch. Immunol. Ther. Exp.(Warsz) v.46 Mechanism of joint destruction in rheumatoid arthritis. Cunnane, G.;K. M. Hummel;U. Muller-Ladner;R. E. Gay;S. Gay
  2. 대한수의학회지 v.34 시호추출물이 면역계세포의 활성에 미치는 영향. 조정곤;김종면
  3. Am. J. Pathol. v.147 Flavonoids inhibit cytokine-induced endothelial cell adhesion protein gene expression. Gerritsen, M. E.;W. W. Carley;G. E. Ranges;C. P. Shen;S. A. Phan;G. F. Ligon;C. A. Perry
  4. Ann. Rheum. Dis. v.52 Molecular and cellular mechanisms of joint destruction in rheumatoid arthritis: Two cellular mechanisms explain joint destruction. Gay, S.;R. E. Gay;W. J. Koopman
  5. Biochem. Biophys. Res. Comm. v.236 Peroxynitrite scanvenging by flavonoids. Haenen, G. R.;J. B. Paqueay;R. E. Korthouwer'A. Bast
  6. FEBS Lett. v.418 Relative bioavailability of the antioxidant flavonoid quercetin from various foods in man. Hollman, P. C.;J. M. van Trijp;M. N. Buysman;M. S. van der Gaag;M. J. Mengelers;J. H. de Vries;M. B. Katan
  7. 대한수의학회지 v.34 화살나물 및 드룹나무 추출물이 면역계세포의 활성에 미치는 영향. 김종면;최민순;조정곤;정영미;박태욱
  8. Yakugaku Zasshi v.115 Screening of phagocyte activators in plants: enhancement of TNF production by flavonoids. Kunizane, H.;H. Ueda;M. Yamazaki
  9. J. food Hyg. Safety v.13 A study on antiulcer effects of Opuntia dillenii haw on stomach ulcer induced by water-immersion stress in rats. Lee, H. J.;Y. W. Lee;J. H. Kim
  10. Scand. J. Rheumatol. v.27 Detection of cytokine mRNA in human, articular cartilage from patients with rheumatoid arthritis and osteoarthritis by reverse transcriptase-polymerase chain reaction. Leistad, L;M. Ostensen
  11. 한국식품개발연구원 보고서 선인장의 성분분석 및 가공식품의 품질개선 시험. 이영철;김흥만;김영언;황금희;안채경
  12. Biochem. Mol. Biol. Int. v.43 Differential expression of ferritin heavy chain in THP-1 cells infected with Mycobacterium bovis BCG. Lim, J. S.;S. H. Lee;E. Lee;Y. Kang;J. W. Kim;J. K. Kim;H. H. Kim;C. Lee;S. J. Kim;G. H. Bai;H. G. Lee;K. D. Kim;T. W. Chung;Y. K. Choe
  13. Cytokine v.1 Characterization of a subclone (D10S) of the D10.G4.1 helper T-cell line which proliferate to attomolar concentrations of interleukin-1 in the absence of absence of mitogens. Orencole, S. F.;C. A. Dinarello
  14. Kor. J. Gerontol. v.9 The effects of Opuntia ficusindica fruit powder on antioxidant parameters in senescence-acclerated mouse (SAM). Paik,S. K.;H. Y. Kim;S. D. Yang;C. W. Song;T. Shin;S. S. Han
  15. Microcirculation v.3 Apigenin inhibits tumor necrosis factor-induced intercellular adhesion molecule-1 upregulation in vivo. Panes, J.;M. E. Gerritsen;D. C. Anderson;M. Miyasaka;D. N. Granger
  16. J. Neuroimmunol. v.56 The subarachnoid space as a site for precursor T cell proliferation and effector T cell selection in experimental autoimmune encephalomyelitis. Shin, T.;T. Kojima;Y. Ishihara;Y. Matsumoto
  17. 한국식물대보감 송주택;정현철;김병무;태희성
  18. J. Neuroimmunol. v.73 Competitive PCR quantification of pro- and anti-inflammatory cytokine mRNA in the encephalomyelitis. Tanuma, N.;T. Kojima;T. Shin;Y. Aikawa;T. Kohji;Y. Ishihara;Y. Matsumoto
  19. Arterioscler. Thromb. Vasc. Biol. v.16 Selective inhibition of tumor necrosis factor-induced vascular cell adhesion molecule-1 gene expression by a novel flavonoid. Lack of effect on transcription factor NF-kappa B. Welle, J.;R. R. Hill;E. Ferguson;L. J. Devall;B. K. Trivedi;R. S. Newton;U. Saxena