생명과학회지 (Journal of Life Science)

  • 제10권4호
  • /
  • Pages.347-353
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  • 2000
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

간장조직의 활성산소 및 그 제거효소에 미치는 누에(Bombyx mori L.) 분말의 영향

Effects of Silkworm (Bombyx mori L.) Powder on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats

  • 최진호 (부경대학교 식품생명공학부 생화학교실) ;
  • 김대익 (부경대학교 식품생명공학부 생화학교실) ;
  • 박수현 (부경대학교 식품생명공학부 생화학교실) ;
  • 김정민 (부경대학교 식품생명공학부 생화학교실) ;
  • 조원기 (조아제약(주)) ;
  • 이희삼 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 류강선 (농촌진흥청 농업과학기술원 잠사곤충부)
  • 발행 : 2000.08.01
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초록

This study was designed to investigate the effects of silkworm (Bombyx mori L.) powder (SWP) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley(SD) male rats(160$\pm$10g) were fed basic diet (control group), and experimental diets(SWP-200 and SWP-400 groups) added 200 and 400 mg/kg BW/day for 6 weeks. Hydroxyl radical (.OH) levels resulted in a consistent decreases (4.0% and 7.2%, 5.0% and 14.1%, respectively) in liver mitochondria and microsomes of SWP-200 SWP-400 groups compared with control group, and O2 radical level was significantly decreased about 12% in liver cytosol of SWP-400 group compared with control group. Lipid peroxide(LPO) levels were significantly decreased (14.4% and 9.1%, respectively) in liver mitochondria and microsomes of SWP-400 group only compared with control group. Oxidized protein (OP) levels were remarkably decreased about 12.7% and 16.3% in liver microsomes only of SWP-200 and SWP-400 groups, but significant difference between liver mitochondria could not obtained. Mn-SOD activities were remarkably increased (15.8% and 25.2%, respectively) in mitochondria of SWP-200 and SWP-400 groups, but significant difference between Cu, Zn-SOD activities in these group could be not obtained. GSHPx activity was significantly increased in liver cytosol of SWP-400 group compared with control group. These results suggest that silkworm powder may play an effective role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in liver membranes.

키워드

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