생명과학회지 (Journal of Life Science)

  • 제10권2호
  • /
  • Pages.196-201
  • /
  • 2000
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  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

복어 장내에서 분리한 신규 해양 미생물을 이용한 Tetrodotoxin 생산

Production of Tetrodotoxin Using Novel Marine Microorganism Isolated from Intestine of Pufferfish

  • 윤성준 (경성대학교 공과대학 식품공학과) ;
  • 송성광 (경성대학교 공과대학 식품공학과) ;
  • 이명자 (서울식품의약품안전청 시험분석실) ;
  • 정동윤 (서울식품의약품안전청 시험분석실) ;
  • 김희숙 (경성대학교 공과대학 식품공학과) ;
  • 김동수 (경성대학교 공과대학 식품공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과)
  • 발행 : 2000.04.01
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초록

The production of tetrodotoxin (TTX) using Vibrio sp. YE-101, a novel marine microorganism isolated from the intestine of pufferfish, was investigated. Culture condition was optimized for the enhanced production of TTX using response surface methodology. The experimental sets of environmental conditions including pH, temperature and NaCl concentration were designed using central composite experimental design. The optimal conditions of pH, temperature and NaCl concentration were determined to be 8.1, 29.2℃, and 2.6% (w/v) respectively. The relative growth extent could be enhanced up to 80%, and final mouse unit (MU) value of TTX was also enhanced up to 87% by response surface optimization.

키워드

참고문헌

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