생명과학회지 (Journal of Life Science)

  • 제10권2호
  • /
  • Pages.188-195
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  • 2000
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  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

천잠 후부 견사선 유래 발현 유전자 꼬리표 작성 및 분석

Analysis of Expressed Sequence Tags Generated from the Posterior Silkgland cDNA Clones of Antheraea yamamai

  • 윤은영 (농업과학기술원 잠사곤충부) ;
  • 구태원 (농업과학기술원 잠사곤충부) ;
  • 강석우 (농업과학기술원 잠사곤충부) ;
  • 이혜원 (농업과학기술원 잠사곤충부) ;
  • 황재삼 (농업과학기술원 잠사곤충부) ;
  • 김호락 (농업과학기술원 잠사곤충부)
  • 발행 : 2000.04.01
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초록

In order to understand molecular events during silk synthesis and provide genetic resources for molecular breeding, we had analyzed the cDNA library constructed from the posterior silkgland of Antheraea yamamai and partially sequenced 276 randomly selected genes from the cDNA library. Database comparisons of the expressed sequence tags (ESTs) revealed that 26 non-redundant clones showed a high similarity with previously identified genes. Among them, 17 clones exhibited a homology with previously identified insect genes and 9 clones were identical to genes that were previously identified from other organisms. A functional categorization showed that silk synthesis-defense- or stress-related genes, as well as genes involved in the metabolic pathways and in the transcriptional or translational apparatus are represented. In this report, the clone (AY479) which had high similarity with fibroin from A. pernyi was particularly analyzed in detail. The AY479 clone was carboxyl terminal region of fibroin. The 472 bp cDNA has 123 amino acids that shared 85% homology with the fibroin from A. pernyi and its deduced peptide had unique feature, that is, sites of alanine rich residues.

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