생명과학회지 (Journal of Life Science)

  • 제10권2호
  • /
  • Pages.182-187
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  • 2000
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

양파(Allium cepa L)의 RAPD 분석조건 최적화에 관한 연구

Optimization of RAPD-PCR Conditions for Onions, Allium cepa L.

  • 정순재 (동아대학교 생명자원과학부) ;
  • 양보경 (동아대학교 생명자원과학부) ;
  • 김익수 (동아대학교 생명자원과학부) ;
  • 박선희 (동아대학교 생명자원과학부) ;
  • 서전규 (경상남도 농업기술원 양파시험장) ;
  • 남재성 (동아대학교 생명자원과학부) ;
  • 김현경 (동아대학교 생명자원과학부) ;
  • 김도훈 (동아대학교 생명자원과학부)
  • 발행 : 2000.04.01
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초록

The optimized RAPD-PCR conditions, which can be utilized as a basic information for the analysis of the genetic characteristics were investigated with four onion varieties, named Changryungdaego, Yeoeuijuhwang, Yakwangju, and Dabonghwang using Operon primers, OPR01 (TGCGGGTCCT) and OPZ20 (ACTTTGGCGG). We tested several concentrations of DNA, primer, and MgCl2, annealing temperature, number of PCR cycle, and presence/absence of pre-heating time at the begining of PCR reation in the 25${mu}ell$ volume. The best RAPD profiles were obtained using 50ng of DNA, 5mM of primer, 1.5mM of MgCl2, 45$^{\circ}C$ of annealing temperature and an absence of pre-heating time. An establishment of the stable and reproducible RAPD-PCR conditions are expected to be useful for the subsequent RAPD-related investigation, such as genetic characterization of the onion strains, re-establishment of phylogenetic relationships and development of new varieties.

키워드

참고문헌

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