Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지

A Gene Encoding Endoplasmic Reticulum Resident 29 kDa Protein is Regulated by TSH-Dependently at the Transcription Level

갑상선자극호르몬에 의한 분자\ulcorner페론 ERp29 유전자의 발현

  • Park, Soo-Jung (Department of Biology, College of Natural Science, Chungnam National University) ;
  • Lee, Woong-Hee (Department of Biology, College of Natural Science, Chungnam National University) ;
  • Goo, Tae-Won (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology RDA) ;
  • Yun, Eun-Young (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology RDA) ;
  • Hwang, Jae-Sam (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology RDA) ;
  • Kim, Ho (Department of Internal Medicine, College of Medicine, Chungnam National University) ;
  • Shong, Min-Ho (Department of Internal Medicine, College of Medicine, Chungnam National University) ;
  • Kwon, O-Yu (Department of Anatomy, College of Medicine, Chungnam National University)
  • 박수정 (충남대학교 자연과학대학 생물학과) ;
  • 이웅희 (충남대학교 자연과학대학 생물학과) ;
  • 구태원 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 윤은영 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 황재삼 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 김호 (충남대학교 의과대학 내과학교실) ;
  • 송민호 (충남대학교 의과대학 내과학교실) ;
  • 권오규 (충남대학교 의과대학 해부학교실)
  • Published : 2000.04.01
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Abstract

This experiment was performed to evaluate the effect of TSH (thyroid-stimulating) on the ERp29 (endoplasmic reticulum resident 29 kDa protein) gene expression in the rat thyrocytes of FRTL-5 cells. Although ERp29 mRNA was constantly expressed, its expression began to increase remarkably from 10-9 M TSH. and its maximum expression was at 5×10-9 M TSH (about 3.5 fold). On the other hand, the effect of TSH on the abundance of ERp29 mRNA started within 6 h, and peaked at 8 h (about 2.5 fold). Actinomycin D (transcription inhibitor) strongly blocked this effect while cycloheximide (translation inhibitor) did not. The half-life of ERp29 mRNA was about 4.5 h in the presence or absence of TSH that was not affected by the stability of ERp29 mRNA. The effect of TSH on the ERp29 gene expression was specific, while other growth factors (transfferin, insulin, and hydrocortisone) did not alter its expression. Our data indicate for the first time that the expression of ERp29 is regulated transcriptionally by TSH in the thyrocytes.

Keywords

References

  1. Proc. Natl. Acad. Sci. USA v.77 Culture of hormone-dependent functional epithelial cells from rat thyroids Ambesi-Impiombato, F. S.;L. A. Parks;H. G. Coon
  2. J. Cell. Physiol. v.181 Immediate early gene expression in dog thyrocytes in response to growth, proliferation, and differentiation stimuli. Deleu, S.;I. Pirson.;F. Clemint.;T. Nakamura;J. E. Dumont;C. Maenhaut
  3. FEBS Lett. v.402 Molecular cloning o ERp29, a novel and widely expressed resident of the endoplasmic reticulum Demmer, J.;C. M. Zhou;M. J. Hubbard
  4. FASEB J. v.5 Glycosidase inhibitors: inhibitors of N-linked oligosaccharide processing Elbein, A. D.
  5. Nature v.355 Protein folding in the cell Gething, M.-J.;J. F. Sambrook
  6. FEBS Lett. v.463 The mammalian Fizzy and Fizzy-related genes are regulated at the transcriptional and post-transcriptional levels Inbal. J.;T. Listobvsky;M. Brandeis
  7. J. Biol. Chem. v.268 Hormonal regulation of thyroglobulin export from the endoplasmic reticulum of cultured thyrocytes Kim, P. S.;P. Arvan
  8. Z. Naturforsch v.54C The endoplasmic reticulum chaperone GRP94 is induced in the thyrocytes by cadmium Kwon, O.-Y.;Y.-J. Kim;Y. Choi;H. Kim;C. Song;M. Shong
  9. J. Biol. Chem. v.269 Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation Kuznetsov, G.;L. B. Chen;S. K. Nigam
  10. J. Biol. Chem. v.268 Transactivation of the grp78 promoter by $Ca^{2＋}$ depletion. A comparative analysis with A23187 and the endoplasmic reticulum $Ca^{2＋}$ -ATPase inhibitor thap-sigargin Li, W. W.;S. Alexandre;X. Cao;A. S. Lee
  11. J. Biol. Chem. v.268 The secretory pathway is normal in dithiothreitol-treated cells, but disulfide-bonded proteins are reduced and reversibly retained in the endoplasmic reticulum Lodish, H. F.;N. Kong
  12. Mol. Cell. Biochem. v.201 Transcriptional modulation of the human complement factor Ⅰgene in HepG2 cells by protein kinase C activation Minta, J.;M. Fung
  13. FEBS Lett. v.431 Oligomerization properties of ERp29, an endoplasmic reticulum protein Mkrtchian, S.;M. Baryshev;O. Matvijenko;A. Sharipo;T. Sandalva;G. Schneider;M. Inglemen-Sundberg
  14. Eur. J. Biochem. v.251 A stress-inducible rat liver endoplasmic reticulum protein, ERp29 Mkrtchian, S.;C. Fang;U. Hellman;M. Inglemen-Sundberg
  15. Thyroid v.9 Hormone-dependent regulation of inter-cellular adhesion molecule-1 gene in FRTL-5 rat thyroid cells Park. E. S.;S. H. You;H. Kim;O.-Y. Kwon;H. K. Rho;B. H. Cho;S. Taniguchi;L. D. Kohn;M. Shong
  16. Croat. Med. J. v.40 Premature termination codon in the aggrecan gene of nanomelia and its influence on mRNA transport and stability Primorac, D.;C. V. Johnson;J. B. Lawrence;M. B. McKinstry;M. L. Stover;M. S. Schanfield;S. Andjelinovic;T. Tadic;D. W. Rowe
  17. Mol. Cell. Biol. v.5 Calcium ionophore A23187 induces expression on glucose-regulated genes and their heterologous fusion genes Resendez, E.;J. W. Attenello;A. Grafsky;C. S. Chang;A. S. Lee
  18. Z. Naturforsch v.54C Identification of genes in a thyroid cell line regulated by thyroid-stimulating hormone(TSH) Shong, M.;Y.-J. Kim;Y. Choi;O.-Y. Kwon
  19. Annu. Rev. Biophys. Biomol. Struct. v.28 Multiprote DNA complexes in transcriptional regulation Wolberger, C.