생명과학회지 (Journal of Life Science)

  • 제10권2호
  • /
  • Pages.150-156
  • /
  • 2000
  • /
  • 1225-9918(pISSN)
  • /
  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

갑상선자극호르몬에 의한 분자\ulcorner페론 ERp29 유전자의 발현

A Gene Encoding Endoplasmic Reticulum Resident 29 kDa Protein is Regulated by TSH-Dependently at the Transcription Level

  • 박수정 (충남대학교 자연과학대학 생물학과) ;
  • 이웅희 (충남대학교 자연과학대학 생물학과) ;
  • 구태원 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 윤은영 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 황재삼 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 김호 (충남대학교 의과대학 내과학교실) ;
  • 송민호 (충남대학교 의과대학 내과학교실) ;
  • 권오규 (충남대학교 의과대학 해부학교실)
  • Park, Soo-Jung (Department of Biology, College of Natural Science, Chungnam National University) ;
  • Lee, Woong-Hee (Department of Biology, College of Natural Science, Chungnam National University) ;
  • Goo, Tae-Won (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology RDA) ;
  • Yun, Eun-Young (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology RDA) ;
  • Hwang, Jae-Sam (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology RDA) ;
  • Kim, Ho (Department of Internal Medicine, College of Medicine, Chungnam National University) ;
  • Shong, Min-Ho (Department of Internal Medicine, College of Medicine, Chungnam National University) ;
  • Kwon, O-Yu (Department of Anatomy, College of Medicine, Chungnam National University)
  • 발행 : 2000.04.01
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초록

This experiment was performed to evaluate the effect of TSH (thyroid-stimulating) on the ERp29 (endoplasmic reticulum resident 29 kDa protein) gene expression in the rat thyrocytes of FRTL-5 cells. Although ERp29 mRNA was constantly expressed, its expression began to increase remarkably from 10-9 M TSH. and its maximum expression was at 5×10-9 M TSH (about 3.5 fold). On the other hand, the effect of TSH on the abundance of ERp29 mRNA started within 6 h, and peaked at 8 h (about 2.5 fold). Actinomycin D (transcription inhibitor) strongly blocked this effect while cycloheximide (translation inhibitor) did not. The half-life of ERp29 mRNA was about 4.5 h in the presence or absence of TSH that was not affected by the stability of ERp29 mRNA. The effect of TSH on the ERp29 gene expression was specific, while other growth factors (transfferin, insulin, and hydrocortisone) did not alter its expression. Our data indicate for the first time that the expression of ERp29 is regulated transcriptionally by TSH in the thyrocytes.

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참고문헌

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