Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Separation of Tetrodotoxin, DHA and EPA from Pufferfish Liver Waste

복어간 폐기물로부터 Tetrodotoxin, DHA 및 EPA의 분리

  • 차병윤 (경성대학교 공과대학 식품공학과) ;
  • 최진석 (경성대학교 공과대학 식품공학과) ;
  • 임정규 (경성대학교 공과대학 식품공학과) ;
  • 이동익 (경성대학교 공과대학 식품공학과) ;
  • 이원갑 (경성대학교 공과대학 식품공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과) ;
  • 김희숙 (경성대학교 공과대학 식품공학과) ;
  • 김동수 (경성대학교 공과대학 식품공학과)
  • Published : 2000.04.01
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Abstract

The present study was undertaken to separate the available components effectively, such as tetrodotoxin(TTX), docosahexaenoic acid(DHA, C22:6,ω-3) and eicosapentaenoic acid (EPA, C20:5,ω -3) from pufferfish liver waste, which are known to have high values as bioactive materials. By using ultrafiltration, it was possible to separate high contents of 68mg TTX from pufferfish liver waste. In contrast, by activated charcoal column, it was to obtain about 54mg TTX. The recovering ratios were 65.3% and 45.0% in the two different methods of ultrafiltration and activated charcoal column, respectively. From the results of HPLC and gas chromatography-mass spectrometry(GC-MS), the obtained toxins were identified to be TTX and its derivatives. In addition, it was also possible to obtain 72.3g DHA and 11.4g EPA from 1kg of pufferfish liver by high performance liquid chromatography (HPLC). These amounts of DHA and EPA were also 17.70% and 1.04% in the total lipid of pufferfish liver oil from analysis of gas chromatography(GC), respectively.

Keywords

References

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