생명과학회지 (Journal of Life Science)

  • 제10권1호
  • /
  • Pages.45-51
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  • 2000
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  • 1225-9918(pISSN)
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  • 2287-3406(eISSN)
한국생명과학회 (Korean Society of Life Science)
권호 표지

버섯파리(Coboldia fuscipes)의 난소발육 및 난황단백질에 관한 연구

Ovarian Development and Yolk Protein of the Mushroom Fly, Coboldia fuscipes (Diptera: Scatopsidae)

  • 김성렬 (동아대학교 생명자원과학대학) ;
  • 최광호 (동아대학교 생명자원과학대학) ;
  • 김익수 (동아대학교 생명자원과학대학) ;
  • 진병래 (동아대학교 생명자원과학대학) ;
  • 손흥대 (동아대학교 생명자원과학대학)
  • 발행 : 2000.02.01
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초록

Ovarian development and yolk protein (YP) of mushroom fly, Coboldia fuscipes, were characterized. C. fuscipes has a pair of ovaries, composed of 130∼140 ovarioles, respectively. Ovarian development begins at 1 day of pupa, and growth of the ovaries continued to become a matured shape at 1 day after emergence. The YP of C. fuscipes identified by SDS-PAGE analysis revealed that the protein is composed of three subunits, designated YP1 (61 kDa), YPS(50 kDa), and YP3 (47 kDa). These three subunits of YP gradually decreased during embryogenesis. The YP was first detected in the 3 day-old pupal ovary and was continually detected up to 2 day-old adult, but not in the hemolymph. Furthermore, Western blot analysis of male and female adult hemolymph and ovary revealed that the antibodies against YP1, YP2, and YP3 reacted with three YP bands in ovary and egg extract, respectively. However, this reactivity was not observed in the male and female hemolymps. Therefore, it is assumed that the UP of C. fuscipes is synthesized in the ovary at 3 days after pupation.

키워드

참고문헌

  1. J. Insect Physiol v.28 Ovarian yolk protein synthesis in Drosophila melanogaster Bownes, M.
  2. Ann. Rev. Entomol v.31 Expression of the genes coding for vitellogenin synthesis in Dorsophila melanogaster. Bownes, M.
  3. Dev. Biol. v.89 The follicle cells are a major site of vitellogenin synthesis in Dorsophila melanogaster. Brennan, M.D.;A.J. Weiner;T.J. Goralski;A.P. Mahowald.
  4. Insect Biochem v.12 Phosphorylation of the vitellogenin polypeptides of Drosophila melanogaster. Brennan, M.D.; A.P. Mahowald.
  5. Comp. Biochem. Physiol. v.88B Vitellogenesis in the stable fly, Stomoxys calcitrans. Chen, A.C.;H.R. Kim;R.T. Mayer;J.O. Norman.
  6. Korea J. Appl. Entomol. v.36 Development characteristics and life cycle of a sciarid fly (Lycoriella sp.) in indoor rearing. Choi, K.H.;H.C. Park;P.D. Kang;S.K. Kang;H.D. Sohn.
  7. Insect Biochem. v.15 Vitellin of Musca domestica quantification and synthesis by fat bodies and ovaries. de Bianchi, A.G.;M. Coutinho;S.D. Pereira;O. Marinotti;H.J Targa.
  8. Insect Biochem. Mol. Biol v.28 Vitellin envelope genes of the yellow fever mosquito, Aedes aegypti. Edwards, M.J;D.W. Severson:H.H. Hagedorn.
  9. Arch. Insect BIochem. Physiol. v.9 Identification and analysis of the major yolk polypeptide from the Caribbean fruit fly, Anastrepha suspensa (Loew). Handler, A.M.;P.D. Shirk.
  10. Invertebr. Reprod. Dev. v.17 Ovarian development and vitellogenesis in the sawfly, Athalia rosae ruficornis Jakovlev (Hymenoptera, Tenthredinidae). ) Hatakeyama, M.;M. Sawa;K. Oishi.
  11. Korean J. Appl. Entomol. Ovarian development and vitellin of mushroom fly, Lycoriella mali (Diptera : Sciaridae).) Kim, S.R.;K. H. Choi;W.J. Yang;I. KIm;B.R. Jin;H.D. Sohn
  12. Comprehensive Insect Physiology, Biochemistry and Pharmacology v.1 Yolk protein Kunkel, J.G.;J.H. Nordin.;G.A. Kerkut(ed.);L.I. Gilbert(ed.)
  13. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Laemmli, U.K.
  14. Korean J. Entomol. v.22 Yolk protein and the oocyte development in Lucilia illustris. Lee, J.J.;H.K. Lee;K.Y. Chang; K.R. Lee.
  15. Ann. Rev. Entomol. v.37 Accumulation of yolk proteins in insect oocytes. Raikhel, A.S.;T.S. Dhadialla.
  16. Comp. Biochem. Physiol. v.86 Two vitellinsvitellogenins of the mediterranean fruit fly, Ceratitis capitata: a comparative biochemical and immunological study. Rina, M.D.;A.C. Mintzas.
  17. Proc. Natl. Acad. Sci. USA v.76 Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Towbin, H.;T. Stahelin;J.Gordon
  18. J Mol. Evol. v.34 The boll weevil vitellogenin gene: nucleotide sequence, structure, and evolutionary relationship to nematode and vertebrate vitellogenin genes. Trewitt, P.M.;L.J. Heilmann;S.S. Degrugillier;A.K. Kumaran.
  19. Insect Mol. Biol. v.6 Cloning and characterization of three Musca domestica yolk protein genes. White, N.M;M. Bownes
  20. Comp. Biochem Physiol. v.9 Subunit composition of vitellin, and concentration profiles of vitellogenin and vitellin in Phormia regina(Meig) following a protein meal. Zou, B.X.;J.G. Stoffolano;J.H. Nordin;C.M. Yin.