Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Ovarian Development and Yolk Protein of the Mushroom Fly, Coboldia fuscipes (Diptera: Scatopsidae)

버섯파리(Coboldia fuscipes)의 난소발육 및 난황단백질에 관한 연구

  • 김성렬 (동아대학교 생명자원과학대학) ;
  • 최광호 (동아대학교 생명자원과학대학) ;
  • 김익수 (동아대학교 생명자원과학대학) ;
  • 진병래 (동아대학교 생명자원과학대학) ;
  • 손흥대 (동아대학교 생명자원과학대학)
  • Published : 2000.02.01
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Abstract

Ovarian development and yolk protein (YP) of mushroom fly, Coboldia fuscipes, were characterized. C. fuscipes has a pair of ovaries, composed of 130∼140 ovarioles, respectively. Ovarian development begins at 1 day of pupa, and growth of the ovaries continued to become a matured shape at 1 day after emergence. The YP of C. fuscipes identified by SDS-PAGE analysis revealed that the protein is composed of three subunits, designated YP1 (61 kDa), YPS(50 kDa), and YP3 (47 kDa). These three subunits of YP gradually decreased during embryogenesis. The YP was first detected in the 3 day-old pupal ovary and was continually detected up to 2 day-old adult, but not in the hemolymph. Furthermore, Western blot analysis of male and female adult hemolymph and ovary revealed that the antibodies against YP1, YP2, and YP3 reacted with three YP bands in ovary and egg extract, respectively. However, this reactivity was not observed in the male and female hemolymps. Therefore, it is assumed that the UP of C. fuscipes is synthesized in the ovary at 3 days after pupation.

Keywords

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