Caspase3-like Death Protease Is Activated in CTLL2 Cells by Interleukin-2 Deprivation

  • Lee, Sang-Han (Biopotency Evaluation Lab, Korea Research Institute of Bioscience & Biotechnology, Korea and Division of Medical Oncology, Columbia University College of Physicians & Surgeons) ;
  • Kwon, O-Yu (Department of Anatomy, College of Medicine, Chungnam National University)
  • 발행 : 2000.12.01

초록

Cytokine deprivation-induced apoptosis can abrogate by the appropriate survival factors. Because the mechanism of Interleukin (IL)-2 deprived apoptotic cell death remains unclear, we here show the apoptosis in CTLL2 cells correlates with an increase of the activity of caspase3-like protease(s). Inhibition of caspase3-like protease(s) with caspase protease inhibitors (Z-VAD, Z-EVD, and Z-LPD) blocks typical apoptotic morphological abnormalities in CTLL2 cells. Interestingly, Bcl-{TEX}$X_{L}${/TEX} protein was decreased by IL-2 deprivation in the cells. These results suggest that caspase3-like protease(s), not caspase1, plays an important role in apoptosis execution of CTLL2 cell death.

키워드