Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Isolation and Characterization of Lacticin 10790, a New Bacteriocin Produced by Lactococcus lactis subsp. cremoris KFCC 10790

  • Joo, Nam-Eok (Department of Chemistry and Biochemistry, Pai-Chai University) ;
  • Kim, Il-Han (Department of Chemistry and Biochemistry, Pai-Chai University) ;
  • Yoo, Jin-Young (Division of Food Biochemistry and Biotechnology, Korea Food Research Institute) ;
  • Lee, Yong-Eok (Department of Biochemistry, Dongguk University)
  • Published : 2000.08.01
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Abstract

A new bacteriocin, named lacticin 10790, was purified from Lactococcus lactis subsp. cremoris KFCC 10790 by sequential adsorption, immobilized metal-affinity, cation-exchange, and $C_{18}$ reverse-phase chromatographies. The molecular mass of the bacteriocin was estimated to be between 3,000 and 3,500 Da. Lacticin 10790 showed a broad antimicrobial spectrum against many gram-positive bacteria. The bacteriocin was stable to heat and in the pH range between 2 and 6. Lacticin 10790 was destroyed by digestion with proteases and exhibited a bactericidal mode of action. An amino acid composition analysis of purified lacticin 10790 revealed a high concentration of hydrophobic amino acids. The N terminus of the bacteriocin was found to be blocked, upon analysis by Edman degradation. The results suggest that lacticin 10790 is a class I bacteriocin.

Keywords

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