Journal of Microbiology and Biotechnology

  • 제10권4호
  • /
  • Pages.529-531
  • /
  • 2000
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Optimized Conditions for In Vitro High Density Encystation of Giardia lamblia

  • Hong, Wook-Sun (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Kim, Kyong-Jpp (Department of Environmental Engineering and Biotechnology, Myongji University) ;
  • Lee, Ki-Say (Department of Environmental Engineering and Biotechnology, Myongji University)
  • 발행 : 2000.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Giardia lamblia, a waterborne parasitic protozoa causing diarrhea and gastroenteritis, is transmitted to humans from untreated and treated water in the form of cysts. The ingestion of G. lamblia cysts is followed by the excystation of the cysts to trophozoites and subsequent colonization of the upper small intestine. In this study, the in vitro conditions for upper small intestine. In this study, the in vitro conditions for G. lamblia encystation were investigated to enhance the efficiency of cyst conversion and the resulting cyst density. The trophozoite of G. lamblia was cultivated to the late exponential growth phase, resulting in a high density of over $6{\times}10^7{\;}cells/ml$. The effects of pH, bile content, and induction time were evaluated; A cyst conversion of over 25% and 107 time were evaluated; A cyst conversion of over 25% and 107 cysts/ml were routinely obtained using the optimized encystation conditions including a slightly slkaline pH, 10 to 15 mg/ml of bile concentration, and 48-50 h of induction time.

키워드

참고문헌

  1. Parasitic Diseases, (3rd ed.) Despommier, D. D.;R. W. Gwadz;P. J. Hotez
  2. J. Immunol. Meth v.202 A comparison of conventional microscopy, immunofluorescence microscopy and flow cytometry in the setection of Giardia lamblia cysts in beaver fecal samples Dixon, B. R.;M. Parenteau;C. Martineau;J. Fourier
  3. Wat. Res. v.32 Bench scale experiments for the evaluation of a membrane filtraition method for the recovery efficiency of Giardia and Cryptosporidium from water Falk, C. C.;P. Karanis;D. Schoenen;H. M. Seitz
  4. Pure. Appl. Chem. v.68 Chlorine in water disinfection Galal-Gorchev, H.
  5. Exp. Parasitol. v.69 Giardia lamblia: The roles of bile, lactic acid, and pH in the composition of the life cycle in vitro Gillin, F. D.;S. E. Boucher;S. S. Rossi;D. S. Reiner
  6. J. Parasitol. v.77 In vitro encystation of Giardia lamblita: Large-scale production of in vitro cysts and strain and clone differences in encystation efficiency Kane, A. V.;H. D. Ward;G. T. Keusch;M. E. A. Pereira
  7. Trans. Royal Soc. Trop. Med. Hyg. v.77 Axenic cultivation of Giardia lamblia in TYI-S-33 medium supplenented with bile Keister, D. B.
  8. Parasitic Protozoa v.10 Giardia in Humans and Animals Kulda, J.;E. Nohynkova;J. P. Kreier(ed.)
  9. Appl. Environ. Microbiol v.57 Giardia and Cryptosposidium spp. in filtered drinking water supplies LeChevallier, M. W.;W. D. Norton;r. G. Lee
  10. Microbiol. Mol. Biol. Rev. v.61 Mechanisms of Giardia lamblia differentiation into cysts Lujan, H. D.;M. R. Mowatt;t. E. Nash
  11. Kor. J. parasitol v.37 Axenic cultivation and characterization of Giardia lamblia isolated from humans in Korea Park, S J.;T. S. Yong;H. W. Yang;D. H. Lee;K. Lee
  12. Appl. Environ. Microbiol. v.53 A new method to determine Giardia cyst viability: Correlation of fluorescein diacetate and propidum iodide staining with animal infectivity Schupp, D. G.;S. L. Erlandsen
  13. Appl. Environ. Microbiol. v.62 An evaluation method for the simultaneous detection of Cryptosporidium oocysts and Giardia cysts from water Shepherd, K. M.;A. P. Wyn-Jones
  14. Yonsei J. Med. Sci. v.16 Resistance of the Ciarida lamblia cysts to various disinfectants Song, J. S.;J. J. Kim;K. T. Lee
  15. J. Microbiol. Biotechnol. v.10 Encystation of Giardia lamblia by high bile and alkaline pH and its ultrastructural changes during encystaion Yong, T. S.;H. W. Yang;K. I. Im;S.-J. Park