Screening and Identification of an Antifungal Pseudomonas sp. That Suppresses Balloon Flower Root Rot Caused by Rhizoctonia solani

  • Ryu, Jae-San (Kyungnam Agricultural Research & Extension Services) ;
  • Lee, Sang-Dae (Kyungnam Agricultural Research & Extension Services) ;
  • Lee, Young-Han (Kyungnam Agricultural Research & Extension Services) ;
  • Lee, Seong-Tae (Kyungnam Agricultural Research & Extension Services) ;
  • Kim, Dong-Kil (Kyungnam Agricultural Research & Extension Services) ;
  • Cho, Soo-Jeong (Department of Agricultural Chemistry, Gyeongsang National University) ;
  • Park, Sang-Ryeol (Department of Agricultural Chemistry, Gyeongsang National University) ;
  • Bae, Dong-Won (Department of Agricultural Chemistry, Gyeongsang National University) ;
  • Park, Ki-Hun (Department of Agricultural Chemistry, Gyeongsang National University) ;
  • Yun, Han-Dae (Department of Agricultural Chemistry, Gyeongsang National University)
  • Published : 2000.08.01

Abstract

A pathogenic fungus causing balloon flower root rot (Platycodon grandiflorum) was isolated from naturally infected roots. The microbial characteristics of the isolated microorganism were similar to those of Rhizoctonia solani. About 500 bacterial species from field soils were screened for a biological agent against the above-mentioned putative pathogen, and several bacteria with the antifungal activity were isolated. Among them, the isolated JS2 was identified as Pseudomonas aeruginosa. This strain showed a broad spectrum of antifungal activity potentially. When the antifungal substance was purified from a broth culture of JS2, it was identified as 2,4-diacetylphloroglucinol (Phl).

Keywords

References

  1. Annu. Rev. Phytopathol. v.6 Mechanism of biological control of soilborn pathogens Baker, R.
  2. Phytochemistry v.28 Antifungal phloroglucinol derivatives and lipophillic flavonoids from Helichrysum decambens Barberan, T.;T. K. Wrayv.
  3. Appl. Environ. Microbiol. v.60 Identification of a genetic locus in Pseudomons aureofaciens involved in fungal inhibition Carruthers, F. L.;A. J. Conner;H. K. Mashanty.
  4. Annu. Rev. Phytopathol. v.31 Making greater use of introduced microorganisms for biological of plant pathogens Cook. R. J.
  5. Appl. Environ. Microbiol. v.63 Role of 2,4-diacetyphloroglucinol in the interactions of the biocontrolPseudomonad strian F113 with the potato cyst nematode Globodera rostochiensis Cronin, D.;Y. Moenne-Loccoz;A. Fenton; C. Dunne;A. N. Dowling;F. Ogara
  6. phytochemistry v.15 c-acetylphloroglucinols from Pseudomonas Fluorescens Douglas, B.;R. P. mabelis;H. Spencer
  7. Curr. Microbiol. v.20 Suppression of Septoria tritici by phenazine-or siderophore-deficient mitants of Peudominas Flaishman, M.;Z. Eyal;C. Voisard;D. Hass.
  8. Enhancing Efficacies of Biological Control: Alternatives for Suppressing Agricultural Pests and Diseases Gullterson, N. I.;W. Howie;T. Suslow
  9. Appl. Environ. Microbiol. v.60 Cloning of genes involved in the synthesis of pyrrolnitrin from Pseudomonas Fluorescens and role of pyrrolnitrin synthesis in biological control of plant disease Hill, D. S.;N. R. Torkewitz;a. M. Morse;C. R. Howell;J. P. Pachlatko;J. O. Decker;J. M. Ligon
  10. Bergy's manual of Determinative Bacteriology, (9th ed. ) Holt, J. G.;J. G. Krieg
  11. J. Microbiol. Biotechnol. v.8 purification and characterization of antistaphylococcal substance from Pseudomonas sp. KUH-001 Hwang, S. Y.;S. H. Lee;K. J. Song;Y. P. Kim;K. Kawahara
  12. Plant Dis. v.77 Evaluation of the Bilog GN microplate system for identification of some plant-pathogenic bacteria Johnes, J. B.;A. R. Chaes;G. K. Harris
  13. Mol. Plant-Microbe Interact v.5 Suppression of root rot disease by Pseudomonas Fluorescens CHA0: Importance of the bacteria secondary metabolite 2,4-diacetylphloroglucinol Keel. C.;U. Schnider;M. Maurhufer;C. Voisard;J. Laville;U. Burger;P. Writhner;D. Haas;G. Defago
  14. J. Microbiol. Biotechnol. v.4 Antifungal mechanism and properties of antibiotic substances produced by Bacillus subtillus YB-70 as a biological control agent Kim, Y. S.;S. D. Kim.
  15. Phytopathology v.70 Effects of rhizobacteria on potato plant development and yirld Kloepper, J. W.;M. N. Schroth;T. D. Miller
  16. Compendium of Medicinal Plant Diseases with Colour Plates Lee, E. J.
  17. Eyakcho-jaebae(Cultivation guide of medicinal crop) Lee. S. T.
  18. J. Microbiol. biotechnol. v.6 Structure determination and biological activities of elaiphylin produced by Streptomyces sp. MCY-846 Lee. S. Y.;M. S. Kim;H. S. Kim;Y. H. Kim;s. D. Hing;J. J. Lee
  19. J. Microbiol. Biotechnol. v.7 Role of siderophores in biocontrol of Fusarium solani and enhanced growth response of bean by Psedomonas fluorescens Lim, H. S.;S. D. Kim
  20. Appl. Environ. Miceobiol. v.58 Contribution of phenazine antibiotic biosynthesis to the ecological competence of fluorescent Pseudomonas in soil habitats Mazzola, M.;R. J. Cook;L. S. Thomashow;D. M. Weller;L. S. Pierson
  21. Khim. Prir. Soedin (Tashk) v.2 Mono-, di- and tri- acetylphloroglucinols from Pseudomonas flurorescens Reddi, T. K.;A. V. Borovkov
  22. J. Sericult. Sci. v.47 A method for isolating the white root rot pathogen, Rosellinia necatrix Berl;in soil Shikata, H.;t. Mitsueda
  23. Methods in Research on Soilborn Phytopathgenic Fungi Singleton, L. L.;J. D. Mihail;C. M. Rush
  24. J. Bacteriol. v.170 Role of a phenazine antibiotic from Pseudomanas fluorescens in biological control of Gaeumanomyces graminis var. tritici. thomashow, L. S.;D. M. Weller
  25. Plant Dis. v.69 Application of a rapid screening test for selection of bacteria suppressive to take-all of wheat Weller, D. M.;B. S.;Zhang;R. J. Cook