Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Falcarindiol, a Polyacetylenic Compound Isolated from Peucedanum japonicum, Inhibits Mammalian DNA Topoisomerase I

  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

A methanol extract of the root of Peucedanum japonicum, used as a medicinal herb, showed an inhibitory effect on mammalian topoisomerase I activity. The methanol extract was suspended in ethyl acetate, and a topoisomerase I inhibitor in the organic soluble fraction was then isolated by silica gel and thin layer chromatography. The topoisomerase I inhibitory compound was indentified as falcarindiol based on the analysis of EI-MS, $^1$H and \ulcornerC NMR spectroscopy. This inhibitory showed cytotoxicity against human leukemia Jurkat T and HL60 cells with an IC\ulcorner value of 7 $\mu\textrm{g}$/ml. These results suggest the possibility of falcarindiol as a new anticancer agent which can be expected to have a synergistic effect on other anticancer drugs. In addition, the present data show that falcarindiol has antifungal, yet not antibacterial, activity.

Keywords

References

  1. J. Nat. Prod v.59 Cytotoxic falcarinol oxylipins from Dendropanax arboreus Bernart, M. W.;J. H. Cardellina;M. S. Balaschak;M. R. Alexander;R. H. Shoemarker;M. R. Boyd
  2. Science v.246 DNA topoisomerase I-targeted chemotherapy of human colon cancer in xenografts Giovanella, B. C.;J. S. Stehlin;M. E. Wall;M. C. Wani;A. W. Nicholas;L. F. Liu;M. Potmesil
  3. J. Biol. Chem. v.268 Induction of mammalian DNA topoisomerase I-mediated DNA cleavage and DNA topoisomerase I-mediated DNA cleavage and DNA winding by bulgarein Fujii, N.;Y. Yamashita;Y. Saitoh;H. Nakano
  4. Biochim. Biophys. Acta v.1262 Eukayotic DNA topoisomerase I. Gupta, M.;A. Fujimori;Y. Pommier
  5. Antibiotics: Mechanism of Action of Antimicrobial and Antitumor Agents v.3 Camptothecin Horwitz, S. B.;J. W. Corcoran(ed.);F. E. Hahn(eds.)
  6. J. Biol. Chem. v.260 Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. Haiang, Y. H.;R. Hertzberg;S. Hecht;L. F. Liu
  7. Chem. Pharm. Bull. v.46 Antiproliferative constituents in Umbelliferae plants. III. Constituents in the root and ground part of Anthriscus sylvestris Hoffm Ikeda, R.;T. Nagao;H. Okabe;Y. Nakano;H. Matsunaga;M. Katano;M. Mori
  8. Chem. Pharm. Bull. v.31 Chemical studies on the constituents of the Chinese crude drug Quiang Huo Kozawa, M.;M. Fukumoto;Y. Matsuyama;K. Baba
  9. J. Microbiol. Biotechnol. v.8 Inhibition of DNA topoisomerase I by cryptotanshinone from Salvia miltiorrhiza Lee, D. S.;S. D. Hong
  10. J. Biol. Chem. v.268 β-Lapachone, a novel DNA topoisomerase I ingibitor with a mode of action different from camptothecin Li, C. J.;L. Averboukh;A. Pardee
  11. Annu. Rev. Biochem. v.58 DNA topoisomerase poisons as antitumor drugs Liu, L. F.
  12. Bioorg. Med. Chem. Lett v.8 Hepatoprotective and nitric oxide production inhibitory activities of coumarin and polyacetylene constituents from the roots of Angelica furcijuga Matsuda, H.;T. Murakami;T. Kageura;K. Ninomiya;I. Toguchida;N. Nishida;M. Yoshikawa
  13. J. Agric. Food Chem. v.44 Antimutagenic activity of falcarindiol from Pseucedanum praeruptorum Miyazawa, M.;H. Shimamura;R. C. Bhuva;S. I. Nakamura;H. Kameoka
  14. Biol. Pharm. Bull. v.21 Antiproliferative constuituents in Umbelligerae plants. II. Screening for polyacetylenes in some Umbelliferae plants, and isolation of panaxynol and falcarindiol from the root of Heracleum moellendorffii. Nakano, Y.;H. Matsunaga;T. Saita;M. Mori;M. Katano;H. Okabe
  15. The Encyclopedia of Wakan-Yaku with Color Pictures v.1 Namba, T.
  16. J. Virol. Methods v.20 Rapid and automated tetrazolium-based colorimetric assay for detection of anti-HIV compounds Pauweis, R.;J. Balzarini;M. Baba;R. Snoeck;D. Schols;P. Herdewijin;J. Desmyster;E. De. Clercq
  17. Nucleic Acids Res v.17 The basis ofr camptothecin enhancement of DNA breakage by eukaryotic topoisomerase I. Porter, S.;J. J. Champoux
  18. Cancer Res v.48 Resistance of human leukemic and normal lymphocytes to drug-induced DNA cleavage and low levels of DNA topoisomerase II. Potmesil, M.;Y. H. Hsiang;L. F. Liu;B. Bank;H. Grossberg;S. Kirschenbaum;T. J. Forlenza;A. Penziner;D. Kanganis;D. Knowles;F. Traganos;R. Silber
  19. Mol. Pharm v.54 Diospyrin, a bisnaphthoquinone: A novel inhibitor of type I DNA topoisomerase of Leishmania donovani Ray, S.;B. Hazra;B. Mittra;A. Das;H. K. Majumder
  20. Semin. Oncol. v.26 Topoisomerase I inhibitors in the treatment of colorectal cancer Rothenberg, M. L.;C. D. Blanke
  21. J. Natl. Cancer Inst. v.85 The current status of camptothecin analogues as antitumor agents Slichenmeyer, W. J.;E. K. Rowinsky;R. C. Donehower;S. H. Kaufna
  22. Biochim. Biophys. Acta. v.909 Recent studies of DNA topoisomerases Wang. J. C.
  23. Annu. Rev. Biochem. v.65 DNA topoisomerases Wang, J. C.
  24. Proc. Natl. Acad. Sci. USA v.85 Involvement of DNA topoisomerase I in transcription of human ribosomal RNA genes Zhang, H.;J. C. Wang;L. F. Liu