Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Cloning and Expression of Inulin Fructotransferase Gene of Arthrobacter sp. A-6 in Escherichia coli and Bacillus subtilis

  • Kim, Hwa-Young (Graduate School of Biotechnology, Korea University) ;
  • Kim, Chan-Wha (Graduate School of Biotechnology, Korea University) ;
  • Choi, Yong-Jin (Graduate School of Biotechnology, Korea University)
  • Published : 2000.04.01
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Abstract

The inulin fructotransferse (depolymerizing) (IFTase, EC 2.4.1.93) gene of Arthrobacter sp. A-6 was cloned and expressed in Escherichia coli and Bacillus subtilis. The IFTase gene consisted of an ORF of 1.311 nucleotides encoding a polypeptide of 436 amino acids containing a signal peptide of 31 amino acids in the N-terminus. The molecular mass of the IFTase based on the nucleotide sequence was calculated to be 46.116 Da. The recombinant E. coli $DH5{\alpha}$ cells expressing the Arthrobacter sp. A-6 IFTase gene produced most of the IFTase intracelularly. In contrast, the recombinant B. subtilis DB 104 carrying the IFTas gene on a B. subtilis-E. Coli expression vector secreted the IFTase into the culture fluid efficiently.

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References

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