Journal of Microbiology and Biotechnology

  • 제10권2호
  • /
  • Pages.201-207
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  • 2000
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Effects of Nutrient Levels on Cell Growth and Secondary Carotenoids Formation in the Freshwater Green Alga, Chlorococcum sp.

  • Liu, Bei-Hui (Department of Nicrobiology, Faculty of Nedicine, The National University of Singapore) ;
  • Haizhang, Dao (Laboratory of Biosignal and Bioengineering, Institute of Molecular Agrobiology) ;
  • Lee, Yuan-Kun (Department of Nicrobiology, Faculty of Nedicine, The National University of Singapore)
  • 발행 : 2000.04.01
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초록

The freshwater green alga Chlorococcum sp. grew on NH_4^{+},{\;}NO_3^{-}$, urea, yeast extract, and peptone as the nitrogen source showing similar pattens of growth and secondary carotenoid (SC) production. However, the most suitable nitrogen source for the induction fo SC was urea. The dffects of nutrient levels (urea, phosphate, sulfate, ferrous iron, and salt) on growth and SC production were stydied by varying the concentration of each nutrient in batch cultures. High biomass production was achieved in cultures containing 20-28 mM urea, 4.8-10 mM phosphate, 1.6 mM sulfate, 70 mM phosphate, 1.6 mM sulfate, 170 mM NACl, and $50{\;}\mu\textrm{M}$ iron. The optimum concentrations of nutrients for biomass and for the SC accumulation in biomass were evaluated and the two media for achieving high biomass production and SC production were thus developed. The extent to which each parameter to stimulate the formation of SC in the alga were varied and the potentially improned SC prodution by manipulating the nutrient levels in the modified media were descussed.

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참고문헌

  1. Plant Physiol. v.72 On the factors which determine massive β-carotene accumulation in the halotolerant alga Dunaliella bardawil Ben-Amotz, A.;M. Avron
  2. Microalgal Biotechnology Dunaliella Borowizka, M. A.;L. T. Borowitzka;M. A. Borowitzka(ed.);L. T. Borowizka(ed.)
  3. Algal Biotechnology Limits to growth and carotenogenesis in laboratory and large-scale outdoor cultures of Dunaliella salina Borowitzka, M. A.;L. T. Borowitzka;T. Stadler(ed.);H. Mollion(ed.);M. C. Verdus(ed.);Y. Karamanos(ed.);H. Mollion(ed.);M. C. Verdus(ed.);Y. Karamanos(ed.);H. Morvan(ed.);D. Chrostiaen(ed.)
  4. J. Appl. Phycol. v.3 Cultures of the astaxanthin-producing green alga Haematococcus pluvialis. 1. Effect of nutrient on growth and cell type Borowitzka, M. A.;J. M. Huisman;A. Osborn
  5. Plant Cell Physiol. v.32 Astaxanthin accumulation in the green alga Haematococcus pluvialis Boussiba, S.;A. Vonshak
  6. Meth. Enzymol. v.213 Enhancement and determination of astaxanthin accumulation in green alga Haematococcus pluvialis Boussiba, S.;F. Lu;A. Vonshak
  7. Phycologia v.6 Development of red pigmentation in Chlorococcum wimmeri (Chlorophyta: Chloroccales) Brown, T. E.;F. L. Richardson;M. L. Vaughn
  8. Bioresource Technol. v.38 Production of astaxanthin from Haematococcus Bubrick, P.
  9. Biotechnol. Lett. v.18 Astaxanthin production from the green alga Haematococcus pluvialis with different stress conditions Cordero, B.;A. Otero;M. Patino;B. O. Arredondo;J. Fabregas
  10. Phytochemistry v.15 Ketocarotenoid biosynthesis by Haematococcus lacutris Donkin, P.
  11. Nature v.57 Studies in carotenogenesis. Ⅱ. Carotenoid synthesis in the alga Haematococcus pluvialis Goodwin, T. W.;M. Jamikorn
  12. Flotow Arch. Mikrobiol. v.20 Conditions governing haematochrome formation in Haematococcus pluvialis Droop, M. R.
  13. Nature v.175 Carotenogenesis in Haemotococcus pluvialis Droop, M. R.
  14. J. Appl. Phycol. v.4 Algal carotenoids 51. Secondary carotenoids 2. Haematococcus pluvialis aplanospores as a source of (3S, 3'S)-astaxanthin esters Grung, M.;F. M. L. D'Souza;M. Borowitzka;S. Liaaen-Jensen
  15. Free Radicals in Biology and medicine Halliwell, B.;J. M. C. Gutteridge(eds.)
  16. Bioresource Technol. v.55 Factors responsible for astaxanthin formation in the Cholorophyte Haematococcus pluvialis Harker, M.;A. J. Tsacalos;A. J. Young
  17. Nutr. Cancer v.19 Studies of immunomodulating actions of carotenoids. Ⅱ. Astaxanthin enhances in vitro antibody production to T-dependent antigens without facilitating poly-clonal B-cell activation Jyonouchi, H.;H. L. Zhang;Y. Tomita
  18. J. Ferment. Bioengng. v.74 Effect of light intensity, light quality and illumination cycle on astaxanthin formation in a green alga, Haematococcus pluvialis Kobayashi, M.;T. Kakizono;N. Nishio;S. Niagai
  19. Appl. Environ. Microbiol. v.59 Enhanced carotenoid biosynthesis by oxidative stress in acetatecarotenoid biosynthsis by oxidative stess in actateinduced cyst cells of a green unicellular alga Haematococcus pluvialis Kobayashi, M.;K. Toshihide;S. Nagai
  20. J. Phycol. v.30 Cell cycle and accumulation of astaxanthin in Haematococcus lacustris (Chlorophyta) Lee, Y. K.;S. Y. Ding
  21. J. Phycol. v.31 Effect of dissolved oxygen partial pressure on the accumulation of astaxanthin in chemostat cultures of Haematococcus lacutris (Chlorophyta) Lee, Y. K.;S. Y. Ding
  22. J. Gen. Microbiol. v.124 Energetics of photosynthetic algal growth: Influence of intermittent illumination in short (40s) cycle Lee, Y. K.;S. J. Pirt
  23. J. Phycol. v.27 Accumulation of astaxanthin in the Haematococcus lacutris Lee, Y. K.;C. W. Soh
  24. Chemicals from Microalgae Production of astaxanthin by Haematococcus Lee, Y. K.;D. H. Zahang;Z. Cohen(ed.)
  25. Biotechnol. Lett. v.21 Composition and biosynthetic pathways of carotenoids in the astaxanthinproduccing green alga Chlorococcum sp. Liu, B. H.;Y. K. Lee
  26. World J. Microbiol. Biotechnol. v.9 Selection and evaluation of astaxanthin-overproducing mutants of Phaffia rhodozyma Mayer, P. S.;J. C. du Preez;S. G. Kilian
  27. Pure Appl. Chem. v.63 Biological functions and activites of animal carotenoids Miki, W.
  28. Plant Physiol. v.81 Partial characterisation of K+ uptake systems in the halotolerant alga Dunaliella salina Pick, U.;A. Ben-Amotz;L. Karni;C. J. Seebergs;M. Avron
  29. J. Plant Physiol. v.144 Accumulation of secondary carotenoids in Chlorella zofingiensis Rise, M.;E. Cohen;M. Vishkautsan;M. Cojocaru;H. E. Gottlieb;S. Arad
  30. Cytologia v.49 Ultrastructural study of Hae matococcus lacustris (Girad.) Rostafinski (Volvocales). I. Some aspects of carotenogenesis Santos, M. F.;J. F. Mesquita
  31. J. Phycol. v.27 Production and selection of high b-carotene mutant of Dunaliella bardewil (Chlorophyta) Shaish, A.;A. Ben-Amotz;M. Avron
  32. U.S. Patent No. 4871551 Pigmentation supplements for animal feed compositions Spencer, K. G.
  33. Aquaculture v.65 Carotenoids in diets for salmonids. Ⅳ. Pigmentation of atlantic salmon with astaxanthin, astaxanthin dipalmitate and canthaxanthin Storebakken, T.;P. Foss;K. Schiedt;E. Austreng;S. Liaaen-Jensen;U. Manzz
  34. Carcinogenesis v.15 Chemoprevention of mouse urinary bladder carcinogenesis by the naturally occurring carotenoid astaxanthin Tanaka, T.;Y. Morishita;M. Suzui;T. Kojima;A. Okumura;H. Mori
  35. J. Phycol. v.27 Effects of inorganic nitrogen availability on algal photosynthesis and carbon metabolism Turpin, D. H.
  36. Plant Cell Physiol. v.31 Lipid bodies in Eremosphaera viridis De Bary (Chlorophyceae) Vechtel, B.;W. Eichenberger;H. G. Ruppel
  37. Microbiology v.142 Selection for carotenogenesis in the yeast Phaffia rhodozyma by dark-generated singlet oxygen William, A. S.;C. Pilar;L. D. Monica;A. J. Eric
  38. Biotechnol. Lett. v.21 Ketocarotenoid production by a mutant of Chlorococcum sp. in an outdoor thbular photobioreactor Zhang, D. H.;Y. K. Lee
  39. J. Appl. Phycol. v.9 Composition and accumulation of secondary carotenoids in Chlorococcum sp. Zhang, D. H.;Y. K. Lee;M. L. Ng;S. M. Phang