Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Identification and Partial Characterization of Cerein BS229, a Bacteriocin Produced by Bacillus cereus BS229

  • Paik, Hyun-Dong (Division of Life Sciences, Kyungnam University) ;
  • Lee, Na-Kyoung (Division of Life Sciences, Kyungnam University) ;
  • Lee, Kwang-Ho (Division of Life Sciences, Kyungnam University) ;
  • Hwang, Yong-Il (Division of Life Sciences, Kyungnam University) ;
  • Pan, Jae-Gu (Bioprocess Engineering Division, Korea Research Institute of Bioscience and Biotechnology (KRIBB), KIST)
  • Published : 2000.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Bacillus cereus BS229 was identified as a bacteriocin producer with a bactericidal activity against Bacillus thuringiensis subsp. Thomsoni BR-40. Bacillus cereus BS229 and cerein BS229, named tentatively as the bacteriocin produced by Bacillus cereus BS229, showed a narrow spectrum of actibity against Gram-positive and Gram-negative bacteria, along with yeast and molds. Production of cerein BS229 in a 5-1 fermenter followed typical kinetics of primary metabolite synthesis. The antibacterial activity of cerein BS229 on sensitive indicator cells disappeared completely by ${\alpha}-chmotrypsin$ or proteinase K, which indicates its proteinaceous nature. Cerein BS229 seemed to be very stable throughout the pH range of 2.0 of 9.0 and it was relatively heat labile, despite the fact that bacteriocin activity was still detected after being boied for 30min. Cerein BS229 actibity has been changed with some of the organic solvents such as toluene, ethanol, and chloroform. Direct detection of cerein BS229 actibity on SDS-PAGE suggested that it had an apparent molecular mass of about 8.2 kDa.

Keywords

References

  1. Appl. Environ. Microbiol. v.56 Plasmid-assocated bacteriocin production by a strain of Carnobacterium piscicola form meat Ahn, C.;M. E. Stiles
  2. J. Biol. Chem. v.263 Strycture and expression of the gene encoding the precursor of subtilin, a small protein antibiotic Banerjee, S.;J. N. Hansen
  3. Antimicrob. Agents Chemother. v.26 Purification and characterization of the Lactocillus acidophilus bacteriocin lactacin B Barefoot, S. F.;T. R. Klaenhammer
  4. J. Dairy Sci. v.71 Inhibitory action of nisin againt Listeria monocytogenes Benkerroum, N.;W. E. Sandine
  5. J. Invertebr. Pathol. v.53 Thuricin: The bacteriocin produced by Bacillus thuringiensis Favret, M. E.;A. A. Youston
  6. Nisin and Novel Lantibiotics Biosynthesis and mechanism of action of nisin and subtilin Hansen, J. N.;Y. J. Chung;W. Liu;M. T. Steen;G. Jung(ed.);H.-G Sahl(ed.)
  7. J. Gen. Microbiol. v.138 Characterization and purification of mesentericin Y104, an anti-Listeria bacteriocin from Leuconostoc mesenteroides Hechard, Y.;B. Derijard;F. Letteller;Y. Cenatiempo
  8. Microbiol. Rev. v.59 Bacteriocins of Gram-positive bacteria Jack, R. W.;J. R. Tagg;B. Ray
  9. Arch. Biochem. v.4 Subtilin, an antibacterial substance of Bacillus subtilis: Culturing condition and properties Jansen, E. F.;D. J. Hirschmann
  10. J. Bacteriol. v.167 Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481 Joerger, M. C.;T. R. Klaenhammer
  11. Appl. Environ. Microbiol. v.58 Analysis of genes invbolved in biosynthesis of the lanibiotic subtilin Klein, C.;C. Kaletta;N. Schnell;K.-D. Entian
  12. Appl. Environ. Microbiol. v.59 Biosynthesis of the lantibiotic subtilin is regulated by a histidine kinase/ response regulator system Klein, C.;C. Kaletta;N. Schnell;K.-D. Entian
  13. J. Inverebr. Pathol. v.15 Thuricin, a bacteriocin produced by Bacillus thuringiensis Krieg, A.
  14. J. Microbiol. Biotechnol. v.9 Identification and characterization of bacteriocin-producing lactic acid bacteria isolated from Kimchi Lee, H.-J.;C.-S. Park;Y.-J. Joo;S.-H. Kim;J.-H. Yoon;Y.-H. Park;I.-K. Hwang;J.-S. Ahn;T.-I. Mheen
  15. Appl. Environ. Microbiol. v.59 Antimicrobial activity of newly identified bacteriocin of Bacillus cereus Naclerio, G.;E. Ricca;M. Sacco;M. D. Felice
  16. Biochem. Biophys. Res. Commun. v.16 Peptide antibiotic subtilin is synthesized va precursor proteins Nishio, C.;S. Komura;K. Kurahashi
  17. Lait v.75 Purification and partial amino acid sequence of propionicin PLG-1, a bacteriocin produced by Propionibacterium thoenii P127 Paik, H.-D.;B. A. Glatz
  18. J. Food Sci. Nutr. v.1 Bacteriocins: Assay, biochemistry, and mode of action Paik, H.-D.
  19. J. Microbiol. Biotechnol. v.6 Purification, characterization, and comparison of bacteriocins Paik, H.-D.;D.-W. Oh
  20. J. Food Prot. v.60 Enhanced bacteriocin production by Propionibacterium thoenii in fed-batch fermentation Paik, H.-D.;B. A. Glatz
  21. J. Ind. Microbiol. Biotechnol. v.19 Identification and partial characterization of tochicin, a bacteriocin produced by Bacillus thuringiensis subsp. tochigiensis Paik, H.-D.;S.-S. Bae;S.-H. Park;J.-G. Pan
  22. Arch. Microbiol. v.154 Caseicin 80: Purification and characterization of a new bacteriocin from Lactobacillus casei Rammelsberg, M.;E. Muller;F. Radler
  23. Bacteriol. Rev. v.40 Bacterocins of Gram-positive bacteria Tagg, J. R.;A. S. Dajani;L. W. Wannamaker
  24. Int. J. Food Microbiol. v.34 Isolation and characterization of Bacillus cereus from pasteurized milk in household refreigerators in The Netherlands Te Giffel, M. C.;R. R. Beumer;P. E. Granum;F. M. Rombouts
  25. J. Bacteriol. v.155 Bacteriocin from Bacillus megaterium ATCC 19213: Comparative studies with megacin A-216 Von Tersch, M. A.;B. C. Carlton
  26. J. Bacteriol. v.155 Megacinogenic plasmids of Bacillus megaterium Von Tersch, M. A.;B. C. Carlton