Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of Heat Shock Protein Protein 70 in Umbilical Vein Endothelial Cells Infected by Staphylococcus aureus

  • Chang, Hyun-Ah (Department of Biology, Sangmyung University) ;
  • Chang, Jun-Keun (School of Electrical Engineering and Computer Science, Seoul National University) ;
  • Kim, Jong-Won (Institute of Biomedical Engineering, Seoul National University) ;
  • Kim, Mal-Nam (Department of Biology, Sangmyung University)
  • Published : 2000.04.01
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Abstract

Environmental stres is known to induce heat shock proteins (HSPs) in eukaryotic cells. However, the induction of HSPs in host cells by microbial infection has not yet been well explained. Staphylococcus aureus (S. aureus) is one of the major pathogens in the pathogenesis of endovascular diseases such as infective endocarditis. In this study, the synthesis of stress-inducible 70 kDa HSP was investigated in the endothelial cells (ECs) after 3 h to 20 h of incubation with S. aureus. The dffect of S. aureus infection on the expression of HSP70 in cultured ECs was analyzed using laser scanning confocal microscopy (LSCM). The increase of HSP70 expression in ECs infected by S. aureus ($10^4{\;}cfu/ml$) for 20 h was 1.1-fold higher than that in heat shock treated ECs and 2.2-fold higher than that in untreated cells. Heat shock is known to induce intranucleus HSP70 expression in mammalian cells, whereas the S. aureus infection induced perinuclear expression in ECs as observed by LSCM. Consequently, the expression of HSP70 in ECs plays an important role in the pathogenesis of endovascular infection.

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References

  1. J. Lab. Clin. Med. v.115 Identification of Staphylococcus aureus binding proteins on isolated procine cardiac valve cells Campell, K. M.;C. M. Johnson
  2. Infection and Immunity v.63 Penetration and damage of endothelial cells by Condida albicans Filler S. G.;N. Jonathan;C. H. Swerdloff;M. L. Peter
  3. Nature v.307 Induction of human vascular endothelial stress fibers by fluid shear stress Franke, R. P.;M. Grafe;H. Schnittler;D. Seiffge;C. Mittermayer
  4. Ann. N. Y. Acad. Sci. v.401 Interaction of platelets and leukocytes with vascular endothelium: In vitro studies Gimbrone, M. A.;M. R. Buchanan
  5. Infection and Immunity v.54 Phagocytosis of Staphylococcus aureus by cultured bovine aortic endothelial cells: Model for postadherence events in endovascular infections Hamill, R. J.;J. M. Vann;R. A. Proctor
  6. FASEB. J. v.11 Inducible nitric oxide synthase-deficient mice have enhanced leukocyteendothelium interactions in endotoxemia Hickey, M. J.;K. A. Sharkey;E. G. Sihota;P. H. Reinhardt;J. D. Macmicking;C. Nathan;P. Kubes
  7. Appl. Microbiol. biotechnol. v.37 Degradation of phenol and phenolic compounds by Pseudomonas putida EKII Hinteregger, C.;R. Leiter, M. Loi;A. Ferschl;F. Streichsbier
  8. J. Microbiol. Biotechnol. v.9 Effect of environmental stress on morphological change of an extremely cadmiumtolerant yeast, Hansenula anomala B-7 Huh, N. E.;N. S. Choi
  9. J. Exp. Med. v.177 Heat shock proteins protect cells from monocyte cytotoxicity: Possible mechanism of self-protection Ja, M.;W. Dorte
  10. J. Biol. Chem. v.23 Differential expression and regulation of hsp70 and hsp90 by phobol esters and heat shock Jacquier-Sarlin, M. R.;L. Jornot;B. S. Polla
  11. J. Clin. Intest. v.52 Culture of human endothelial cells derived from umbilical veins Jaffe, E. A.;R. L. Nachman;C. G. Becker;R. Minick
  12. J. Lab. Clin. Med. v.121 Staphylococcus aureus binding to cardiac endothelial cell is partly mediated by a 130 kilodalton glycoprotein Johnson, C. M.
  13. Exp. Cell. Res. v.15 Nigrosin as a dye for differentiating live and dead ascites cell Kaltenbach, J. P.;M. H. Kaltenbach;W. B. Kyons
  14. Infection and Immunity v.61 Phagocytosis of induced a selective stress response in junal monocytemacrophage: Modulation by macrophge differentiaion and by iron Kantengwa, S.;B. S. Polla
  15. Immunol. Today v.11 Heat shock proteins and the immune response Kauffman, S. H. E.
  16. Infection and Immunity v.60 Hsp70 synthesis in Schwann cells in responses to heat shock and infection with Mycobacterium leprae Mistry, Y.;D. B. Young;R. Mukherjee
  17. J. Bacteriol. v.171 Metal ion uptake by plasmid-free metal sensitive Alclalgents eutrophus Nies, D. H.;S. Silver
  18. Infection and Immunity v.61 Expression of 65 and 67 kilodalton heat regulated proteins and 70-kilodalton heat shock cognate protein of leishmania donovani in macrophages Rey-Ladino, J. A.;N. E. Reiner
  19. J. Biol. Chem. v.265 Heat shock proteins Schlesinger, M.
  20. Proc. Natl. Acad. Sci. USA v.91 Host cell responses to Listeria monocytogenes infected include differential transcription of host genes involved in signal transduction Schwan, W. R.;W. Goebel
  21. J. Med. Microbiol. v.40 Virulence factors involved in the pathogenesis fo bovine intramammary infections due to Staphylococcus Stra, L.;B. Poutrel
  22. Biochem. Biophys. Res. Commun. v.206 Possible correlation between DNA damage induced by hydrogen peroxide and translocation of heat shock 70 protein into the nucleus Tetsuya, A. E.;T. Konishi;K. Higashi
  23. J. Clin. Invest. v.85 A human endothelial cell membrane protein that binds Staphylococcus aureus in vitro Tompkins, D. C.;V. B. Hatcher;D. Patel;G. A. Orr;L. L. Higgins;F. D. Lowy
  24. Infection and Immunity v.55 Ingestion of Staphylococcus aureus by bovine endothelial cells results in time- and inoculum-dependent damage to endothelial cell monolayers Vann, J. M;R. A. Procter
  25. Cell v.36 HSP70; nuclear concentration during environmental stress and cytoplasmic storage during recovery Velazquez, J.;S. Lindquist
  26. J. Biol. Chem. v.259 Nuclear and nucleolar localization of the 72,000-dalton heat shock protein in heat-shocked mammalin cells Welch, W. J.;J. R. Feramisco
  27. Clinical Infectious Disease v.25 Polyclonal Staphylococcus endocarditis Wijngaerden, E. V.;W. E. Peeterman;S. V. Lierrde;J. V. Eldere
  28. Biochem. Biophys. Res. commun. v.200 Oxidized-LDL induce the expression of hsp70 in human endothelial cells Zhu, W.;P. roma;A. L. Catapano