Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Expression of Modified Green Fluorescent Protein in Suspension Culture of Taxus cuspidata

  • Kim, Chang-Heon (Department of Genetic Engineering, Kyung Hee University) ;
  • Kim, Kyung-Il (Department of Genetic Engineering, Kyung Hee University) ;
  • Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University)
  • Published : 2000.02.01
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Abstract

The suspension cells of Taxus cuspidata were transformed with Agrobacterium tumefaciens harboring binary vector pCAMBIE1302 encoding mgfp. Transient transfection efficiency was compared by using the fluoremetric measurement. The transient transfection efficiency was improved by transformation with DMSO and/or sonication treatment. Optimum conditions for DMSO and sonication treatment were 3% and 30sec, respectively. selection and maintenance of transformed cells were continued for 3 months. An insertion of the mgfp gene in transformed cells was detected by PCR and an expression of GFP confirmed by the western blot analysis.

Keywords

References

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