Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of an Elastase Inhibitor Produced by Streptomyces lavendulae SMF11

  • Lee, Hyun-Sook (Department of Microbiology, Seoul National University) ;
  • Jin, Wook (Department of Microbiology, Seoul National University) ;
  • Kang, Sung-Gyun (Department of Microbiology, Seoul National University) ;
  • Hwang, Yoon-Sook (Department of Microbiology, Seoul National University) ;
  • Kho, Yung-Hee (Enzyme Inhibition Research Unit, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Kye-Joon (Department of Microbiology, Seoul National University)
  • Published : 2000.02.01
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Abstract

An elastase inhibitor, SMFEI02, was isolated from culture broth of Streptomyces lavendulae SMF11. The inhibitor was purified by ultrafiltration followed by XAD-7 column and Dowex-1 anion-exchange chromatographies, and preparative HPLC. The molecular formula was determined to be $C_{14}H_{16}N_2O_2$ (MW244) by HRFAB-MS analysis. The inhibitor was identified to be a diketopiperazine cyclo(S-Phe-S-Pro) by the optical rotation value and MNR spectral data, and showed inhibitory activities for trypsin, chymotrypsin, cathepsin B, and papain as well as elastase with the Ki values ranging from 1.78mM to $2.86{\;}\mu\textrm{m}$. The inhibition showed a competitive mode for elastase, chymotrypsin, and cathepsin B, whereas it showed a noncompetitive mode for trypsin and papain.

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References

  1. J. Nat. Prod. v.58 New and known diketopiperazines from the Caribbean sponge, Calyx cf. Adamczeski, M.;A. R. Reed;P. Crews
  2. Biochem. J. v.193 Mouse macrophange elastase. Purification and characterization as a metalloproteinase Banda, M. J.;Z. Werb.
  3. Methods Enzymol. v.144 Elastin degradation. Banda, M. J.;Z. Werb;J. H. McKerrow.
  4. Am. J .Respir. Crit. Care Med v.150 Protease-antiprotease imbalance in the lungs of children with cystic fibrosis Birrer, P.;N. G. McElvanay;A. R udeberg;C. Wirz Sommer;S. Liechti-Gallati;R. Kraemer;R. Hubbard;R. G. Cystal.
  5. Bull. Agric. Chem. Soc. Jpn. v.24 Studies on the metabolic products of Rosellinia necatrix Berlese. Part Ⅰ. Isolation and characterization of several physiologically active neutral substances. Chen, Y.-S.
  6. Lab. Invest. v.19 The role of elastase in acute hemorrhagic pancreatitis in man Geokas. M. C.;H. Rinderknecht;V. Swansson;B. J. Haverback.
  7. J. Lab. Clin. Med. v.82 The induction of emphysema with elastase. Ⅰ. The evolution of the lesion and the influence of serum. Kaplan, P. D.;C. Kuhn;J. A. Pierce.
  8. Kor. J. Microbiol. v.33 Screening and identification of Actinomycetes producing novel elastase inhibitor. Lee, B. K.;H. I. Kang;K. J. Lee
  9. J. Am. Chem. Soc. v.56 The determination of enzyme dissociation constants. Lineweaver, H.;D. Burk.
  10. Anal. Chem. v.31 Use of dinitrosalicylic acid reagent for determination of reducing sugar. Miller, G.
  11. J. Biol. Chem. v.258 Amino acid sequence at the reactive site of human$ α_1$-antichymotrypsin Morii, M.;J. Travis.
  12. J. Org. Chem. v.45 Structure of elasnin, a novel elastase inhibitor containing an α-pyrone ring Nakagawa, A.;H. Ohno;K. Myano;S. Omura
  13. J. Atibiot. v.31 Isolation and characterization of elasnin, a new human graulocyte elastase inhibitor produced by a strain of Streptomyces. Ohno, H.;T. Saheki;J. Awaya;A. Nakagawa;S. Omura.
  14. J. Med. Chem. v.28 Inhibition of human leukocyte elastase, porcine pancreatic elastase, and cymotrypsim by elasnin and other 4-hydroxy-2-pyrones Spencer, R. W.;L. J. Copp;J. R. Pfister.
  15. Proc. Natl. Acad. Sci. v.85 Maculosin, a host-specific phytotoxin for spotted knapweed from Alternaria alternata Stierle, A. C.;J. H. Cardellina Ⅱ;G. A. Strobel.
  16. J. Antibiot. v.26 Elastatinal, a new elastase inhibitor produced by actinonycetes. Umezawa, H.;T. Aoyagi;A. Okura;H. Marishima;T. Takeuchi;Y. Okami.
  17. Annu. Rev. Microbiol. v.36 Low-molecular-weight enzyme inhibitors of microbial origin. Umezawa, H.
  18. Eur. Repir. J. v.8 Digestion of proteoglycans in porcine pancreatic elastase-induced emphysema in rats van de Lest, C. H.;E. M. Versteeg;J. H. Veerkamp;T. H. van Kupevelt.
  19. J. Am. Chem. Soc. v.98 Cyclic peptides.15. Lanthanide-assisted $13^C$ and $1^H$ NMR analysis of preferred side-chain rotamers in proline-containing cyclic dipeptides. Young, P. E.;V. Madison;E. R. Blout.