Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Cloning and Nucleotide Sequence Analysis of the aroD Gene from Salmonella typhi KNIHI100

Salmonella typhi KNIH100으로부터 aroD 유전자의 클로닝과 염기서열 분석

  • 길영식 (충북대학교 자연과학대학 생명공학부) ;
  • 전형규 (충북대학교 자연과학대학 생명공학부) ;
  • 신희정 (충북대학교 자연과학대학 생명공학부) ;
  • 김영창 (충북대학교 유전공학연구소)
  • Published : 2000.09.01
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Abstract

Sulmonella gyhi is one of important causes of human enteric infections. S @phi I

장티푸스는 Salmonella typhi 에 의해 유발되는 장감염성 질환으로 사람과 동물에 공통되는 질병이다. 본 연구에서는 기 보고된Salmonella typhi KNH100의 염색체 DNA로부 터 방향족 아미노산의 생합성에 관여하는 효소인 3-dehydroquinate hydratase(3- dehydroquinate)를 암호화하는 aroD 유전자를 포함하는 약 3.2 kb의 Sal I 절편을 pSAL62 이라 명명하였다. 클로닝된 재조합 plasmid인 pSAL61에는 ATG 개시코돈과 TGA 종결코돈 을 포함하는 759 염기로 구성된 aroD 유전자가 위치하고 있었다. 또한 S. typhi Ty2, Shigella dysenteriae, 그리고 Escherichia coli 등 다른 장내 세균의 aroD 유전자와 상동성을 비교하여 본 결과 각각 90%, 72.7% 그리고 73%의 상동성을 나타내었다.

Keywords

References

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