미생물학회지 (Korean Journal of Microbiology)

  • 제36권2호
  • /
  • Pages.103-108
  • /
  • 2000
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  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

한국에서 분리된 전염성 조혈괴저 바이러스의 non-virion (NV) 단백질의 유전자 클로닝 및 바이러스 증식에서의 역할

Cloning of the non-virion (NV) of a Korean Isolate of Infectious Hematopoietic Necrosis and Identification of the Role of the NV in IHNV Replication

  • 문창훈 (울산대학교 생명과학부) ;
  • 조화자 (울산대학교 생명과학부) ;
  • 윤원준 (울산대학교 생명과학부) ;
  • 박정재 (울산대학교 생명과학부) ;
  • 박정민 (울산대학교 생명과학부) ;
  • 김현주 (울산대학교 생명과학부) ;
  • 도정완 (국립수산진흥원 병리과) ;
  • 이주양 (울산대학교 생명과학부) ;
  • 임채렬 (울산대학교 생명과학부)
  • 발행 : 2000.06.01
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초록

한국에서 분리된 전염성 조혈괴저바이러스(infectious hematopoietic necrosis virus, IHNV)인 IHNV-PRT의 non-viron(NV)단백질을 암호화하고 있는 cDNA를 클로닝하여 이들의 염기서열을 분석하였다. NV는 336bpzmrl의 open reading frame을 포함하였으며 이로부터 111개의 아미노산 서열을 외국에서 분리된 IHNV들과 비교 분석한 결과 90-95%의 상동성을 보였다. 이러한 사실은 INHV의 NV단백질 유전자들은 IHNV의 strain에 관계없이 매우 보존되어 있음을 나타내준다. Northern blotting을 사용하여 NV의 발현을 측정한 결과 감염 후 20 시간분터 발현이 증가함을 확인 힐수 있었다. NV가 바이러스의 증식에 필요한지의 여부를 확인하기 위하여 바이러스 유전자의 antisense DNA를 사용하여 바이러스 증식 억제에 관한 실험을 수행하였다. Glycoprotein (G)의 antisense DNA를 처리한 경우 바이러스의 증식이 거의 억제된 반면 NV에 대한 antisense DNA를 처리한 경우 바이러스 증식에 거의 변화가 없었다. 이로부터 배양중인 세포가 있어서 NV는 증식에 필수적이지 않은 것으로 판단된다.

We have cloned and analyzed cDNA coding for non-virion (NV) protein of the m V - P R T The NV gene contained 336 bp open readmg frame and encoded a protein of 11 1 amino acids with a molecular weight of 13.2 kDa. The deduced amino acid sequence of NV of IHNVPRT was found to be 90-95% identical to those of foreign isolates of IHNV. These results indicate that NV gene of the MNV is highly conserved among &ifferent strains of THNV Northern blot analyses revealed that the levels of NV gene expression were strongly elevated after 20 h post-infection. In order to identify the role of NV in the replication of MNV in fish cells, IHNVinfected cells were treated with antisense oligonucleotides. While IHNV-PRT exposed to glycoprotein (G) antisense oligonucleotide showed severely reduced growth, the growth of virus exposed to NV antisense oligonucleotide was not affected by NV antisense oligonucleotide, which suggests that NV is not essential for replication of IHNV in fish cells.

키워드

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