Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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P22-Based Challenge Phage Constructs to Study DNA-Protein Interactions between the $\sigma$54-Dependent Promoter, dctA, and Its Transcriptional Regulators

  • Kim, Euhgbin (Department of Biology and Institute of Bioscience and Biotechnology, Yonsei University ) ;
  • Kim, Daeyou (Department of Genetic Engineering , Youngdong University) ;
  • Lee, Joon-Haeng (Department of Ophthalmology and The institute of Vision Research, Yonsei University College of Medicine )
  • Published : 2000.09.01
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Abstract

A challenge phage system was used to study the DNA-protein interaction between C4-dicarboxylic acid transport protein D(DCTD) or $\sigma$54, and a $\sigma$54 -dependent promoter, dctAp. R. meliloti dctA promoter regulatory region replaced the Omnt site on the phage. S. typhimurium strains overproducing either DCTD or $\sigma$54 directed this challenge phage towards lysogency, indicating that DCTD or E$\sigma$54 recognized the dctA promoter on the phage and repressed transcription of the ant gene. These challenge phage constructs will be useful for examining interactions between DCTD(or $\sigma$54) and the dctA promoter region.

Keywords

References

  1. Proc. Natl. Acad. USA v.72 Histidine regulation in Sal -monella typhimurium;an activator attenuator model of gene regulation Artz S.W.;J.R. Broach
  2. Nucleic Acids Res v.19 Influence of a mutation in the putative nucleotide binding site of the nitrogen regulatory protein NTRC on its positive control function Austin S.;C. Kundrot;R. Dixon
  3. Genetics v.114 General selection for specific DNA-binding activities Benson N.;P. Sugiono;S. Bass;L.V. Medelman;P. Youderian
  4. Annu. Rev. Genet v.20 Regulation of nitrogen fixation genes Gussin G.N.;C.W. Ronson;F.M. Ausubel
  5. EMBO J. v.8 Transcrip-tional activation of the Klebsiella pneumoniae nifLA promoter by NTRC is face-of -the-helix dependent and the activator sta-bilizes the interaction of σ54-RNA polymerase with the pro-moter Minchin S.D.;S. Austin;R.A. Dixon
  6. J. Mol. Biol v.210 In vivo studies on the interaction of RNA polymerase-σ54 with the Klebsiella pneumoniae and Rhizobium melioti nifH promoters Morett E.;M. Buck
  7. Cell v.50 Initiation of transcription at the bacterial glnAp2 promoter by purified E. coli components is facilitated by enhancers Ninfa A.J.;L.J. Reitzer;B. Magasanik
  8. J. Biol. Chem v.266 Purification of the alternative sigma factor, σ54, from Salmonella typhimurium and characterization of σ54-holoenzyme Popham D.;J. Keener;S. Kustu
  9. Proc. Natl. Acad. Sci. USA. v.85 Probing the Escher-ichia coli glnALG upstream activation mechanism in vivo Sasse-Dwight S.;J.D. Gralla
  10. J. Microbiol v.37 Transcriptional induction of a carbon starvation gene during other starvation and stress challenges in Pseudomonas putida MK1;a role of carbon starvation gene in general starvation and stress responses Subramnian C.;H.S. Lee;Y. Kim
  11. Microbiol. Rev v.42 Molecular genetics of bacteriophage P22 Susskind M.M.;D. Botstein