Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Continuous Synthesis of Escherichia coli GroEL at a high Temperature

  • Published : 2000.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

GroEL is a typical molecular chaperone. GroEL synthesis patterns at various culture temperatures in Escherichia coli were investigated in this study. No significant differences in the amount of GroEL produced from the chromosome were found at 30 and 37$^{\circ}C$. However, GroEL production increased 3.4-fold at 42$^{\circ}C$. GroEL synthesis was not transient but continuous at 42$^{\circ}C$, although most heat shock gene expression is known to be transient. To understand the role of the groEL structural gene, a groE promoter-lacZ fusion was constructed. Interestingly , while transcriptional fusion is not thermally inducible, it is inducible by ethanol, suggesting that the secondary structure of the groEL transcript is involved in thermal regulation of the groEL gene. Secondary structures of groE mRNA at 37 and 42$^{\circ}C$ were compared using the computer program RNAdraw. Distinct structures at the two temperatures were found, and these structures may be related to a high level of GroEL expression at 42$^{\circ}C$.

Keywords

References

  1. Proc. Natl. Acad. Sci. USA v.82 Consensus sequence for Esheri-chia coli heat shock gene promoters Cowing D.W.;J.C.A. Bardwell;E.A. Craig.;C. Woolford;R.W. Hendrix;C.A. Gross
  2. Cell v.90 In vivo observation of polypeptide flux through the bacterial chaperonin system Ewalt K.L.;J.P. Hendrix;W.A. Houry;F.U. Hartl
  3. Nature v.337 GroeE heat-shock proteins promote assembly of foreign prokaryotic ribulose biphosphate carboxylase oligomers in Escherichia coli Goloubinoff P.;A.A. Gatenby;G.H. Lorimer
  4. Nature v.333 Homologous and bacterial proteins chaperone oligomeric protein assembly Hemmingsen S.M.;C. Woolford;S.M. van der Vies;K. Tilly;D.T. Dennis;C.P. Georgopoulos;R.W. Hendrix;R.J. Ellis
  5. J. Bacteriol v.139 Levels of major proteins of Escherichia coli during growth at different temperatures Herendeen S.L.;R.A. VanBogelen;F.C. Neidhardt
  6. Biotechnol. Techniques v.11 Chloramphenicol acetyltransferase expression of Escherichia coli increased at 42°C Kim S.J.;H.Y. Jeon;H.B. Kim
  7. J. Microbiol. Biotechnol v.10 Stress responses of the Escherichia coli groE promotor Kwak Y.;S. Kim;K. Lee;H. Kim
  8. A short course in bacterial genetics Miller J.H.
  9. Nature v.344 Heat-shock proteins DnaK and GroEL facilitate export of LacZ hybrid proteins in E. coli Phillips G.J.;T.J. Silhavy
  10. Mol. Microbiol v.12 Post-tran-scriptional regulation of the groELl gene of Streptomyces Servant P.;C.J. Thomson;P. Mazodier
  11. Proc. Natl. Acad. Sci. USA v.79 Genetic control of heat-shock protein systhesis and its bearing on growth and thermal resi-tance in Escherichia coli Yamamori T.;T. Yura
  12. Nucleic Acids Res v.21 Heat induc-tion of sigma32 synthesis mediated by mRNA secondary struc-ture;a primary step of the heat shock response in Escherichia coli Yuzawa H.;H. Nagai;H. Mori;T. Yura