BMB Reports
- Volume 33 Issue 2
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- Pages.166-171
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- 2000
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- 1976-670X(eISSN)
Temperature-Dependent Expression of Escherichia coli Thioredoxin Gene
- Lee, Jin-Joo (Division of Life Sciences, Kangwon National University) ;
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Park, Eun-Hee
(College of Pharmacy, Sookmyung Women's University) ;
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Ahn, Ki-Sup
(Department of Environment Managemental, Chonan College of Foreign Studies) ;
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Lim, Chang-Jin
(Division of Life Sciences, Kangwon National University)
- Published : 2000.03.31
Abstract
Thioredoxin is a multifunctional protein that is ubiquitous in microorganisms, animals and plants. Previously, the expression of the Escherichia coli thioredoxin gene (trxA) was found to be negatively regulated by cAMP. In the present study, the effect of temperature on the expression of the E. coli trxA gene was investigated. In order to examine the temperature effect, the fusion plasmid pCL70 that harbors the E. coli trxA P1P2 promoter was used. The other two fusion plasmids, pJH3 and pMH521 that were constructed in different vectors which harbor the E. coli trxA P2 promoter, were also used. When the E. coli strain MC1061/pCL70 was grown in a rich medium at