Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지

Effects of Silkworm(Bombyx mori L..) Powder on Lipofuscin, Acetylcholine and Its Related Enzyme Activities in Brain of Rats

뇌조직의 리포푸신, 아세틸콜린 및 그 관련효소 활성에 미치는 누에분말의 영향

  • 최진호 (부경대학교 식품생명공학부 생화학교실) ;
  • 김대익 (부경대학교 식품생명공학부 생화학교실) ;
  • 박수현 (부경대학교 식품생명공학부 생화학교실) ;
  • 김정민 (부경대학교 식품생명공학부 생화학교실) ;
  • 조원기 (조아제약(주)) ;
  • 이희삼 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 류강선 (농촌진흥청 농업과학기술원 잠사곤충부)
  • Published : 2000.12.01
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Abstract

This study was designed to investigate the effect of silkworm (Bombyx mori L.) powder on lipofuscin, acetylcholine (ACh) and its related enzyme activities in brain of rats. Sprague-Dawley (SD) male rats ($160{\pm}10g$) were fed basic diet (control group), and experimental diets (SWP-200 and SWP-400 groups) added 200 and 400 mg/kg BW/day for 6 weeks. In case of liver membranes, lipofuscin (LF) levels resulted in a slight decreases (4.6% and 11.5%, respectively) in SWP-200 and SWP-400 groups compared with control group. But in case of barin as the most sensitive organ, LF levels were remarkably inhibited about 16.7% and 20.0% in SWP-200 and SWP-400 groups compared with control group. There were no significant differences in acetylcholine (ACh) syntheses as a very important neurotransmitter, and choline acetyltransferase (ChAT) activities as a synthesis enzyme of ACh, and acetylcholinesterase (AChE) activities as a hydrolysis enzyme, which were concerned in transmission of neuron through synapses in brain of SWP-200 and SWP-400 groups compared with control group. Monoamine oxidase-B (MAO-B) activities were significantly inhibited (about 10.2%) in brain of SWP-400 groups compared with control group. These results suggest that inhibiting effects of LF accumulation and MAO-B activity of silkworm powder (SWP) may play a pivotal role in attenuating a various age-related changes for improvement of brain function.

Keywords

References

  1. Korean J. Nutr. v.31 no.7 Effects of silkworm powder on blood glucose and lipid levels in NIDDM(type-II) patients. Cho, M. R.;R. W. Choue;S. H. Chung;J. W. Ryu
  2. Korean J. Nutr. v.31 no.7 A studu of folk remedies in type-II diabetic patients. Cho, M. R.;R. W. Choue
  3. Free Rad. Biol. & Med. v.18 no.2 Brain synaptosomal aging: Free radicals and membrane fluidity. Choi, J. H.;B.P. Yu
  4. Korean J. Seric. Sci. v.41 no.3 Effects of mulbery leaf extract on oxygen radicals and their scavenger enzymes in serum of SD rats. Choi, J. H.;D. I. Kim;S. H. Park;D. W. Kim;J. S. Lee;K. S. Ryu;W. C. Lee
  5. Korean J. Seric. Sci. v.41 no.3 Effects of silkworm powder on oxygen radicals and their scavenger enzymes in serum of SD rats. Choi, J. H.;D. I. Kim;S. H. Park;D. W. Kim;J. S. Lee;H. S. Lee;K. S. Ryu
  6. Korean J. Seric. Sci. v.41 no.3 Effects of silk fibroin powder on oxygen radicals and their scavenger enzymes in serum of SD rats. Choi, J. H.;D. I. Kim;D. W. Park;Park,S. H.;D. W. Kim;J. S. Lee;Y. W. Lee
  7. Functional anti-diabetic drink. Choi, J. H.;President of RDA
  8. Functional anti-diabetic drink. Choi, J. H.;President of RDA
  9. Korean J. Seric. Sci. v.42 no.1 Effects of silkworm (Bombyx mori L.) powder on lipofescin, acetylcholine and Its related enzyme activities in brain of SD Rats. Choi, J. H.;D. I. Kim;S. H. Park;D. W. Kim;J. S. Lee;H. S. Lee;K. S. Ryu
  10. Bull. K.H. Pharma. Sci. v.24 Blood glucose lowering effect of silkworm. Chung, S. H.;J. H. Yu;E. J. Kim;K. S, Ryu
  11. Korean J. Seric. Sci. v.39 no.1 Effect of silkworm extect on intestinal α-glycosidase cativity in mice administered with a high carbohydrate-containing diet. Chung, S. H.;M. S. Kim;K. S. Ryu
  12. Biochem. Pharmacol. v.7 A new and rapid colorimeteric determination of acetylcholinesterase activity. Ellman,G. L.;K. O. Cuurtney;V. Andrres;R. M. Featherstone
  13. Anal. Biochem. v.52 Measurement of fluorescent lipid peroxidation products in biological systems and tissues. Fletcher, B. L.;C. J. Dillard;S. A. L. Tappel
  14. Ecol. Prog, Ser. v.19 Evidence of variation of cholinesterase activity in fishes along a pollution gradient in the north sea. Mar. Galgani, F.;G. Bocquene;Y. Cadiou
  15. Neuropharmacol. v.26 no.6 Comlarison of the effects of two inhibitors on brain cholinesterase. Hallak, M.;E. A. Giacobini
  16. Proc. Natl. Acad. Sci. v.84 Correlation of 1-metnyl-4-phenyl-1,2,3,6-tetrahydropyridine neurotoxicicty with blood-brain barrier monoamine oxidase activity. Kalaria, R. N.;M. J. Mitchell;S. Harik
  17. Korean J. Seric. Sci. v.40 no.1 Effect of several sericultural products on blood glucose lowering for alloxan-induced hyperglycemic mice. Lee, H. S.;K. S. Chung;S. Y. Kim;K. S. Ryu;W. C. Lee
  18. J. Biol. Chem. v.193 Protein measurement with the Folin-Phenol reagent. Lowry, O. H.;N. J. Roseborough;L. A. Farr;R. J. Randall
  19. Colored Illustrations of WAKANYAKU v.II Namba, T.
  20. J. Japan Soc. Nutr. Food Sci. v.45 no.1 Effect of disaccharidase inhibitor,AO-128, on postprandial hyperglycemia in rats. Odaka, H.;n. Miki;H. Ikeda;T. Matsuo
  21. Agric. Chem. Biotech. v.41 no.2 Cytotoxic activity of Bombyx mori and Morus alba derived materials against human tumor cell lines Park, I. K.;J. O. Lee;H. S. Lee;K. Y. Seol;Y. J. Ahn
  22. Korean J. Seric. Sci. v.39 no.1 An activity of lowering blood-glucose levels according to preparative conditions of silkworm powder. Ryu, K. S.;H. S. Lee;S. H. Chung;P. D. Kang
  23. J. New Remedies & Clinics v.47 no.1 Significance of peptidoglycan in patients with chronic liver diseases. Shiomi, S.;D. Habu;T. Takeda;S. Nishiguchi;T, Kuroki;T.Tanaka;K. Tsuchida;S. Yamagami
  24. Principles and procedures of statistics. Steel, R. G. D.;J. H. Torrie