Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Purification and Characterization of Chitinase from a Marine Bacterium, Vibrio sp. 98CJ11027

  • 박신혜 (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • 이정현 (Microbiology Laboratory, Korea Ocean Research & Development Institute) ;
  • 이홍금
  • Published : 2002.12.01
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Abstract

Chitin-degrading marine bacterial strain 98CJ11027 was isolated from bryozoa from the coastal area of Cheju Island, Korea, and identified as a member of the genus Vibrio. The molecular mass of the main extracellular chitinase (chitinase I), purified from strain 98CJ11027, was estimated to be 98 kDa. The optimal condition for chitinase I activity is pH 6.0 and 45℃. The activity was inhibited by $Fe^+2$ and$Cu^+2$. Chitinase I displayed the hydrolysis type of chitobiosidase and catalyzed reversed hydrolysis leading to the synthesis of tetraacetylchitotetraose.

Keywords

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