부자이중탕과 보중익기탕의 억제성 및 흥분성 신경전달 물질에 의하여 활성화되는 이온통로 조절작용

Modulation of Bujaijung-tang and Bojungikgi-tang on Inhibitory and Excitatory Neurotransmitters Activated Ion Channels

  • 이혜정 (경희대학교 동서의학대학원 침구경혈학부) ;
  • 서정철 (경희대학교 한의과대학 침구학교실) ;
  • 이재동 (경희대학교 한의과대학 침구학교실) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실) ;
  • 이충열 (경원대학교 한의과대학 생리학교실) ;
  • 정주호 (경희대학교 의과대학 약리학교실) ;
  • 신민철 (경희대학교 의과대학 생리학교실) ;
  • 김현배 (경희대학교 의과대학 생리학교실) ;
  • 김연정 (경희대학교 의과대학 생리학교실) ;
  • 김창주 (경희대학교 의과대학 생리학교실)
  • Lee, Hye-Jung (Department of Acupunture & Meridianology, Graduate School of East-West Medicine Science, Kyung Hee University) ;
  • Seo, Jung-Chul (Department of Acupunture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Jae-Dong (Department of Acupunture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ee-Hwa (Department of Meridianology, College of Oriental Medicine, Se Myung University) ;
  • Lee, Choong-Yeol (Department of Physiology, College of Oriental Medicine, Kyung Won University) ;
  • Chung, Joo-Ho (Department of Pharmacology, College of Medicine, Kyung Hee University) ;
  • Shin, Min-Chul (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Hyun-Bae (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Youn-Jung (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Chang-Ju (Department of Physiology, College of Medicine, Kyung Hee University)
  • 투고 : 2000.10.27
  • 심사 : 2000.11.10
  • 발행 : 2000.12.20

초록

To research the characteristics of ion currents induced by Bujaijung-tang and Bojungikgi-tang, nystatin-perforated patch clamp technique under voltage-c(amp condition was used. Periaqueductal gray neuron was dissociated from Sprauge-Dawley rat, 10-15 days old. Cytotoxicity of Bujaijung-tang and Bojungikgi-tang showed incubation time and concentration dependent manner. Ion current activated by Bujaijung-tang and Bojungikgi-tang were inhibited by bicuculline and strychnine and CNQX. It can be suggested that Bujaijung-tang and Bojungikgi-tang modulate inhibitory and excitatory neurotransmitters-, GABA, glycine and non-NMDA, acticvated ion channels. Modulatory effect of Bujaijung-tang and Bojungikgi-tang was more greater in inhibitory neurotransmitters. Low concentration of Bujaijung-tang which dose not elicit ion current itself, activated GABA and glycine induced chloride currents. In this study, we can found that the activation of Bujaijung-tang and Bojungikgi-tang on non-NMDA subtypes of glutamate receptor is its major action mechanism and can be used as very effective Herb treatment on Myasthenia gravis patient.

키워드

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