Modulation of Bujaijung-tang and Bojungikgi-tang on Inhibitory and Excitatory Neurotransmitters Activated Ion Channels

부자이중탕과 보중익기탕의 억제성 및 흥분성 신경전달 물질에 의하여 활성화되는 이온통로 조절작용

  • Lee, Hye-Jung (Department of Acupunture & Meridianology, Graduate School of East-West Medicine Science, Kyung Hee University) ;
  • Seo, Jung-Chul (Department of Acupunture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Jae-Dong (Department of Acupunture & Moxibustion, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Ee-Hwa (Department of Meridianology, College of Oriental Medicine, Se Myung University) ;
  • Lee, Choong-Yeol (Department of Physiology, College of Oriental Medicine, Kyung Won University) ;
  • Chung, Joo-Ho (Department of Pharmacology, College of Medicine, Kyung Hee University) ;
  • Shin, Min-Chul (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Hyun-Bae (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Youn-Jung (Department of Physiology, College of Medicine, Kyung Hee University) ;
  • Kim, Chang-Ju (Department of Physiology, College of Medicine, Kyung Hee University)
  • 이혜정 (경희대학교 동서의학대학원 침구경혈학부) ;
  • 서정철 (경희대학교 한의과대학 침구학교실) ;
  • 이재동 (경희대학교 한의과대학 침구학교실) ;
  • 김이화 (세명대학교 한의과대학 경혈학교실) ;
  • 이충열 (경원대학교 한의과대학 생리학교실) ;
  • 정주호 (경희대학교 의과대학 약리학교실) ;
  • 신민철 (경희대학교 의과대학 생리학교실) ;
  • 김현배 (경희대학교 의과대학 생리학교실) ;
  • 김연정 (경희대학교 의과대학 생리학교실) ;
  • 김창주 (경희대학교 의과대학 생리학교실)
  • Received : 2000.10.27
  • Accepted : 2000.11.10
  • Published : 2000.12.20

Abstract

To research the characteristics of ion currents induced by Bujaijung-tang and Bojungikgi-tang, nystatin-perforated patch clamp technique under voltage-c(amp condition was used. Periaqueductal gray neuron was dissociated from Sprauge-Dawley rat, 10-15 days old. Cytotoxicity of Bujaijung-tang and Bojungikgi-tang showed incubation time and concentration dependent manner. Ion current activated by Bujaijung-tang and Bojungikgi-tang were inhibited by bicuculline and strychnine and CNQX. It can be suggested that Bujaijung-tang and Bojungikgi-tang modulate inhibitory and excitatory neurotransmitters-, GABA, glycine and non-NMDA, acticvated ion channels. Modulatory effect of Bujaijung-tang and Bojungikgi-tang was more greater in inhibitory neurotransmitters. Low concentration of Bujaijung-tang which dose not elicit ion current itself, activated GABA and glycine induced chloride currents. In this study, we can found that the activation of Bujaijung-tang and Bojungikgi-tang on non-NMDA subtypes of glutamate receptor is its major action mechanism and can be used as very effective Herb treatment on Myasthenia gravis patient.

Keywords

References

  1. Science v.182 Neuromuscular junction in myastheniagravls: Decreased acetylcholine receptors Fambrough, D.M.;Drachman, D.B.;Satyamurti, S.
  2. Physioi Rev. v.60 Immunology of acetylcholine receptors in relation to myastheniagravis Vincent, A.
  3. 정교황제내경소문 홍원식(교합편찬)
  4. 동의재활의학과학 전국한의과대학 재활의학과학교실(편저)
  5. 實用中醫藥臨床治療手冊 陳可冀(總編)
  6. Trends Nerosci v.10 The acetyichoiinereceptor molecule: allosteric sites and the ion channel Changeux, J.P.;Revah, F.
  7. 동의생리학회지 v.14 附子理中湯과 補中益氣湯에 의한 이온전류와 nicotinic acetylcholine 수용체 김창주;김연정;정성현;이충열;류재환;이혜정
  8. Ann Rev Neurosci v.17 $\GABA_A$ receptor channels Macdonald, L.;Olsen, R.W.
  9. Brain Res. v.736 Effects of GABA and glycine receptor antagonists on the activity and PAG-induced inhibition of rat dorsal horn neurons Peng, Y.B.;Lin, Q.L.;Willis, W.D.
  10. Neuroscience v.97 Effects of persistent nociception of periaqueductal gray glycine release Maione, S.;Marabese, I.;Sca Rossi, F.;Berrino, L.;Palazzo, E.;Trabace, L.;Rossi, F.
  11. Neuron v.7 Sustained potentiation of NMDA receptor-mediated glutamate responses through activation of protein kinase C by a $\mu$ opioid Chen, L.;Huang, L.Y.M.
  12. J Neurophysiol v.77 Modulation of high-voltage activated $Ca^{2+}$ channels in the rat periaqueductal gray neurons by $\mu$- type opioid agonist Kim, C.J.;Rhee, J.S.;Akaike, N.
  13. 대한한의학회지 v.17 중증근무력증에 대한 임상적 연구 채병윤
  14. Ann Rev Physio. v.57 Mechanism of activation of glutamate receptors and time course of excitatory synaptic currents Edmonds, B.;Gibb, A.J.;Colquhoun, D.
  15. Trends Neurosci. v.18 Excitotoxicity and the NMDA receptor-still lethal after eight years Rothman, S.M.;Olney, J.W.
  16. J Neurochem v.57 $Ca{^2+}$ -independent release of glutamate during in vitro anoxia in isolated nerve terminals Rubio, I.;Torres, M.;Miras-Portugal, M.T.;Sanchez-Prieto, J.
  17. Trend Neurosci v.16 The molecular biology of glutamate receptor channel Seeburg, P.H.
  18. J Physiol v.399 The role of divalent cations in the N-methyl-D-aspartate responses of the mouse central neurons in culture Ascher, P.;Nowak, L.
  19. Nature v.325 Glycine potentiates the NMDA responses in culture mouse brain neurons Johnson, J.W.;Ascher, P.
  20. 簡明方劑辭典 江克明;包明蕙(편저)
  21. J Mol Neurosci v.7 Apoptosis induced in neuronal cultures by either the phosphatase inhibitor okadaic acid or the kinase inhibitor staurosporine is attenuated by isoquinolinesulfonamides H-7. H-8 and H-9 Cagnoli, C.M.;Kharlamov, E.;Atabay, C.;Uz, T.;Manev, H.