Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

The Effect of Salviae Radix on Oxidat-Inhibition of Phosphate Uptake in Renal Proximal Tubular Cells

단삼약침액(丹蔘藥鍼液)이 신장(腎臟) 근위세뇨관세포(近位細尿管細胞)에서 산화제(酸化劑)에 의한 인산(燐酸)의 이동억제(移動抑制)에 미치는 영향(影響)

  • Lee, Ho-Dong (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong Eui University) ;
  • Youn, Hyoun-Min (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong Eui University) ;
  • Jang, Kyung-Jeon (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong Eui University) ;
  • Song, Choon-Ho (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong Eui University) ;
  • Ahn, Chang-Beohm (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong Eui University)
  • 이호동 (동의대학교 한의과대학 침구경혈학교실) ;
  • 윤현민 (동의대학교 한의과대학 침구경혈학교실) ;
  • 장경전 (동의대학교 한의과대학 침구경혈학교실) ;
  • 송춘호 (동의대학교 한의과대학 침구경혈학교실) ;
  • 안창범 (동의대학교 한의과대학 침구경혈학교실)
  • Received : 2000.08.12
  • Accepted : 2000.08.26
  • Published : 2000.09.20
Download PDF
⟨ Previous Next ⟩

Abstract

This study was undertaken to determine if Salviae Radix (SR) exerts protective effect against oxidant-induced inhibition of phosphate uptake in renal proximal tubular cells. Membrane transport function and cell death were evaluated by measuring phosphate uptake and trypan blue exclusion, respectively, in opossum kidney (OK) cells, an established proximal tubular cell line. $H_2O_2$ was used as a model oxidant. $H_2O_2$ inhibited the phosphate uptake in a dose-dependent manner over the concentration range of 0.1-0.5 mM. Similar fashion was observed in cell death. However, the phosphate uptake was more vulnerable to $H_2O_2$ than cell death, suggesting that $H_2O_2$-induced inhibition of phosphate uptake is not totally attributed to cell death. Decreasedphosphate uptake was associated with ATP depletion and inhibition of $Na^+$-pump activity as determined by direct inhibition of $N^+-K^+$-ATPase activity. When cells were treated with $H_2O_2$ in the presence of 0.05% SR, the inhibition of phosphate uptake and cell death induced by $H_2O_2$ was significantly attenuated. SR restored ATP depletion and decreased $Na^+-K^+$-ATPase activity, and this is likely responsible for the protective effect of SR on decreased phosphate uptake. The protective effect of SR was similar to the $H_2O_2$ scavenger catalase. SR reacts directly with $H_2O_2$ to reduce the effective concentration of the oxidant. The iron chelator deferoxamine prevented the inhibition of phosphate uptake and cell death induced by $H_2O_2$, suggesting that $H_2O_2$-induced cell injury is resulted from an iron-dependent mechanism. These results indicate that SR exerts the protective effect against $H_2O_2$-induced inhibition of phosphate uptake by reacting directly with $H_2O_2$ like the $H_2O_2$scavenger enzyme catalase, in OK cells. However, the underlying mechanism remains to be explored.

Keywords

References

  1. 臟腑辨證論治 金完熙;崔達永
  2. 東醫腎系內科學 杜鎬京
  3. Physiol. Res. Hydroperoxide metabolism in mammalian organs Chance, B.;Sies, H.;Boveris, A.
  4. Free Radicals in Biology Free radical mechanisms of lipid damage and consequences for cellular membranes Mead, J. F.;W Pryer(Ed.)
  5. J. Clin. Invest. v.74 Oxygen free radicals in ischemic acute renal faiture in the rat Paller, M.S.;Hoidal, J.R.;Ferris, T.F.
  6. Lab. Invest. v.51 Evidence for the role of oxygen free radicals in acute nephrotoxic nephritis Rehan, A.;Johnson, K. J.;Wiggins, R. C.;Kunkel, R. G.;Ward, P. A.
  7. J. Clin. Invest. v.81 Evidence of the role of hydroxil radical in gentamicin-Induced acute renal failure in rats Walker, P. D.;Shah, S. V.
  8. Science v.175 Microsomal lipid peroxidation :Morphological characterization Arstila, A. U.;Smith, M. A.;Trump, B. F.
  9. Am. J. Physiol v.265 Oxidant-induced alterations in glucose and phos-phate transport in $LLC-PK_1$ cells: mechanisms of injury Andreoli, S. P.;McAteer, J. A.;Seifert, S.A.;Kempson, S. A.
  10. Am. J. Physiol. v.254 Depression of membrane-bound $Na^+-K^+-ATPase$ activity induced by free radicals and by ischemia of kidney Kako, K.;Kato, M.;Matsuoka, T.; Mustapha, A.
  11. Free Rad. Biol. Med. v.19 Oxidant stress and endothelial membrane transport Elliott, S, J.;Koliwad, S. K.
  12. Am. J. Physiol. v.264 Lipid peroxidation of the brush-border membrane: mombrane physical properties and glucose transport Jourd'Heuil, D.;Vaanen, P.;Meddings, J. B.
  13. 臨床本草學 辛民敎
  14. 中草藥學 上海中醫學院(編)
  15. 漢方醫藥大事典 陳存仁
  16. 大韓韓醫學會誌 v.19 no.1 $H_2O_2$에 의한 腎臟 細胞 損傷에 대한 丹蔘抽出物의 防止效果 金尙範;鄭智天
  17. 大韓韓方內科學會誌 v.18 no.1 Oxidant에 의한 腎臟細尿管物質 移動系의 障碍에 대한 丹蔘의 效果 金尙範;鄭智天
  18. 大田大學校大學院碩士學位論文 丹蓼의 免疫 機能에 대한 實驗的 硏究 羅京洹
  19. Anal. Biochem. v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing Bradford, M. M.
  20. J. Biol. Chem. v.66 The colorimetric determination of phosphorus Fiske, C. H.;SubbaRow, Y.
  21. Toxicol. Appl. Pharmacol. v.141 Differential effect of $Ca2^+$ on oxid ant-induced lethal cell injury and alte rations of membrane transport fun ctional integrity in renal cortical slices Kim, Y. K.;Kim, Y. H.
  22. Kid. Int. v.38 Reactive oxygen molecule mediated injury in endothelial and renal tubuiar epithelial cells in vitro Andreoli, S. P.;McAteer, J. A.
  23. 晋臟學 서울대학교 의과대학(편)
  24. 臨床病理檢査法 李三悅
  25. 大韓韓醫學書誌 v.17 no.1 胡桃藥鍼液이 腎臟細胞에서 oxidant에 의한 損傷에 미치는 影響 金永海;金甲成
  26. 本草綱目 欽定四庫全書 41 李時珍
  27. 本草秘要解析 楊東喜
  28. 아세아 본초학 金泰姬(外)
  29. 本草學 李尙仁
  30. 中藥學 顔正華
  31. 中國醫藥學報 v.6 no.2 丹蔘對 急性醫衰的療效觀察及機理硏究 張步振(外)
  32. 中成藥 v.15 no.11 丹蔘對老齡小鼠 SOD和 LPO的 影響 郭忠興(外)
  33. 大韓鍼灸學會誌 v.16 no.2 丹蔘藥鍼이 Rhabdomyolysis에 의한 急性腎不全에 미치는 影響 朴世貞(外)
  34. Am. J. Physiol. v.259 Oxidative stress in isoiated rat renal proximal and distal tubular cells Lash, L. H.;Tokarz, J. J.
  35. Arch.Biochem.Biophys. v.270 Mitochondrial damage as a mechanism of cell injury in the killing of cultured hepatocytes by tort-butyl hydroperoxide Masaki, N.;Kyle, M.E.;Serroni, A.; Farber, J.L.
  36. Toxicol. Appl. Pharmacol v.78 Organic hydroperoxide induced lipid peroxidation and cell death in isolated hepatocytes Rush, G.F.;Gorski, J.R.;Ripple, M.G.;Sowinski, J.;Bugelski, P.; Hewitt, W.R.
  37. Am.J.Physiol v.268 Role of lipid peroxidation in $H_2O_2$-induced renal ep ithelial $LLC-PK_1$ cell injury Salahudeen, A.K.
  38. Lab. Invest. v.62 Biology of disease: Mechanlsms of cell injury by activated oxygen species Farber, J, L.;Kyle, M. E.;Coleman, J. B.
  39. Arch. Biochem. Biophys. v.284 Inhibition of iodoacetamide and tbut-ylhy droperoxide toxicity in $LLC-PK_1$ cells by antioxidants: a role for lipid peroxidation in alkylation induced cyt otoxicity Chen, Q.;Stevens, J.L.