대한예방한의학회지 (Journal of Society of Preventive Korean Medicine)

대한예방한의학회 (Society of Preventive Korean Medicine)
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지실 추출물의 세포독성과 항균효과

Cytotoxity and Antimicrobial Effects of the Extract of Poncirus trifoliata

  • 양현옥 (원광보건대학 미용피부관리과) ;
  • 오현주 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 박낭규 (원광대학교 한의학전문대학원 천연물학교실) ;
  • 최은영 (김천과학대학 피부미용과) ;
  • 이현옥 (원광보건대학 치위생과) ;
  • 양은영 (건일제약(주) 중앙연구소) ;
  • 천현자 (원광대학교 한의학전문대학원 해부학교실) ;
  • 정순량 (우석대학교 자연과학대학 화학과) ;
  • 이강민 (전북대학교 자연과학대학 생명과학부) ;
  • 백승화 (원광대학교 한의학전문대학원 천연물학교실)
  • Yang Hyun-Ok (Department of Cosmetics, Wonkwang Health Science College) ;
  • Oh Hyun-Ju (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Park Nang-Kyu (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Choi Eun-Young (Department of Beauty and skin care, Kimcheon Science College) ;
  • Lee Hyun-Ok (Department of Hygiene, Wonkwang Health Science College) ;
  • Yang Eun-Yeong (Kunhil Pharmaceutical Co., Ltd.) ;
  • Chun Hyun-Ja (Department of Anatomy, Professional Graduate School of Oriental Medicine, Wonkwan University) ;
  • Chung Soon-Ryang (Department of Chemistry, College of Natural Sciences, Chonju Woosuk University) ;
  • Lee Kang-Min (Department of Molecular Biology, Junbuk University) ;
  • Baek Seung-Hwa (Department of Natural Products, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 발행 : 2000.12.29
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초록

This study was carried out to evaluate cytotoxic effects of Poncirus trifoliata Raf. extract on lymphocytic leukemia tumor (L1210) cell lines. Disruptions in cell organelles were determined by 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay The comparison of Ic50 Values of Poncirus trifoliata Raf. extract in L1210 cell lines showed that their susceptibility to these fractons decreased in the following order: adriamycin > Fr.4> Fr. 6> Fr. 5> Fr. 3> Fr. 1> Fr. 2 by the MTT assay. In order to develop an antumicrobial agent, Poncirus trifoliata Raf. was extracted wit ethanol, and then it was fractionated with several mobile phase. The antitumor activities of fractions of the ethanol soluble extract was investigated. The minimal inhibitory concentrations (MIC) of fractions of the ethanol soluble extract of Poncirus trifoliata Raf. against microorganisms were also examined. Antimicrobial activities of ampicillin and ketoconazole as references were compared to those of fractions of the ethanol soluble extract of Poncirus trifoliata Raf. The antimicrobial activities of all fractions from the extract had growth inhibition activities against gram-positive bacteria, gram-negative bacteria and fungi $(MIC\;>\;200{\mu}g/ml)$. These results suggest that fraction 4 of the ethanol soluble extract of Poncirus trifoliata Raf. possessed the most antitumorous agent.

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참고문헌

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