Journal of Society of Preventive Korean Medicine (대한예방한의학회지)

Society of Preventive Korean Medicine (대한예방한의학회)
권호 표지

Antioxidant Effects of Sagunja-Tang (Sijunzi-Tang)

사군자탕(四君子湯)의 항산화(抗酸化) 효과(效果)

  • Lee Yong-Tae (Dept. of Physiology, College of Oriental Medicine, Dong-Eui University) ;
  • Cho Su-In (Dept. of Herbology, College of Oriental Medicine, Dong-Shin University) ;
  • Kim Young-Kyun (Dept. of Internal Medicine, College of Oriental Medicine, Dong-Eui University)
  • 이용태 (동의대학교 한의과대학 생리학교실) ;
  • 조수인 (동신대학교 한의과대학 본초학교실) ;
  • 김영균 (동의대학교 한의과대학 내과학교실)
  • Published : 2000.12.29
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : This study was carried out to research antioxidant effects of Sagunja-Tang(SA) through in vitro and vivo experiments, and tried to investigate the relation between oxidation of tissues and deficiency of Qi. Methods and results : HPLC analysis of glycyrrhizine - known to be the main compound of Radix Glycyrrhizae - was done to certify the quality of SA. Chemiluminescence was initiated by adding tort-butyl hydroperoxide (t-BHP) to rabbit polymorphonuclear leukocytes (neutrophils), and generated reactive oxygen species (superoxide anion) decreased significantly by SA as dose dependent manner. Cell injury during 60 minutes tissue incubation was initiated by adding t-BHP, a hydrophobic hydroperoxide and $H_2O_2$, an water soluble oxidant to rat renal cortical and liver slices. Percentage cell death and lipid peroxidation were estimated by measuring lactate dehydrogenase (LDH) and malondialdehyde (MDA), a product of lipid peroxidation. t-BHP induced % cell death of renal cortical slices and lipid peroxidation of renal cortical and liver slices were decreased significantly by SA. SA decreased significantly % cell death and lipid peroxidation of renal cortical and liver slices induced by $H_2O_2$, too. Acute renal and liver injury induced by $HgCl_2\;and\;CCl_4$, which initiated from free radical, were applied to mice and metabolic data were obtained. Data showed protective effects of SA on acute renal injury caused by decrease of glomerular filtration. SA protected acute liver injury too. Conclusions Through this study, we found that SA have antioxidant effects and tissue oxidation was similar to deficiency of Qi. And further studies have to be followed to certify the mechanisms.

Keywords

References

  1. 太平惠民和制局方 陳師文(等編)
  2. 天眞處方解說 成輔社(編譯)
  3. 虛證論 索延昌
  4. 精校黃帝內經素問 洪元植(校合編纂)
  5. 精校黃帝內經靈樞 洪元植(校合編纂)
  6. 신물질탐색 신물질탐색연구회(편저)
  7. J. Pharmacol. Exp. Ther. v.258 no.2 A potent inhibitor of lipid peroxidation with activity in experimental brain injury and ischemia Hall, E.D.;Braughler, J.M.;Yonkers, P.A.;Smith, S.L.;Linseman, K.L.;Means, E.D.;Scherch, H.M.;Von Voigtlander, P.F.;Lahti, R.A.;Jacobson, E.J.
  8. Biochem. J. v.205 Superoxide forming NADPH-oxidase preparation of pig polymorphonuclear leukocytes Wakeyama, H.;Takeshige, K.;Takayanagi, R.;Minakami, S.
  9. Anal. Biochem. v.86 Determination of malonaldehyde precusor in tissue by thiobarbituric acid test Uchiyama, M.;Mihara, M.
  10. Anal. Biochem. v.72 no.1976 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dry binding Bradford, M.M.
  11. 臨床本草學 辛民敎
  12. 中藥名方藥理與應用 珍奇
  13. J. Amer. Geriatr. Soc. v.20 The Biologic clock:the mitochondria? Harman, D.
  14. Free Rad. Res. v.21 Low mitochondrial free radical production per unit $O_2$ consumption can explain the simultaneous presence of high longevity and high aerobic metabolic rate in birds Barja, G.;Cadenas, S.;Rojas, C.
  15. Science v.273 Oxidative stress, caloric restriction, and aging Sohal, R.S.;Weindruch, R.
  16. Free RAD. Biol. Med. v.21 Aging and oxidative stress:modulation by dietary restriction Yu, B.P.
  17. Lab. Invest. v.62 Biology of disease:Mechanisms of cell injury by activated oxygen species Farber, J.L.;Kyle, M.E.;Coleman, J.B.
  18. J. Clin. Invest. v.73 The respiratory burst of phagocytes Babior, B.M.
  19. Biochem. Biophys. Acta. v.853 The $O_2$ forming NADPH-oxidase of the phagocytes:nature, machanism of activation and function Rossi, F.
  20. 釜山醫大學術誌 v.29 no.1 호중구의 Superoxide 생성 Oxidase의 활성화에 대한 연구 윤석환;정병선
  21. Methods in Enzymology v.105 Oxygen Radicals in Biological System Packer, L.
  22. 프리라디칼 金永坤;金永杓
  23. 병리학 대한병리학회
  24. Basic Pathology Syanley L. Robbins;Vinay Kumar
  25. Angrew. Chem. Int. Ed. v.25 Biochemistry of oxidant stress Sies, H.
  26. Physiol. Rev. v.59 Hydroperoxide metabolism un mammalian organs Chance, B.;Sies, H.;Boveris, A.
  27. Science v.175 Microsomal lipid peroxidation:Morphological characterization Arstila, A.U.;Smith, M.A.;Trump, B.F.
  28. Am. J. Physiol. v.254 Depression of membrane-bound $Na{^+}-K{^+}$-ATPase activity induced by free rqadicals and by ischemia of kidney Koko, K.;Kato, M.;Matsuoka, T.;Mustapha, A.
  29. Am. J. Physiol. v.265 Oxidant-induced alterations in glucose and phosphate transport in $LLC-PK_1$ cells. Mechanisms of injury Andreoli, S.P.;McAteer, J.A.;Seifert, S.A.;Kempson, S.A.
  30. J. Microencapsul. v.13 no.4 Monitoring by cis-parinaric fluorescence of free radical induced lipid peroxidation in aqueous liposome susoensions Di Giulio, A.;Saletti, A.;Oratore, A.(et al.)
  31. In Mechanisms of Cell Injury:Implications for Human Health Mechanism of chemical-induced glomerulonephritis Druet, P.;Hirsch, F.;Pelletier, L.(et al.);Fowler, B.A.(Ed.)
  32. Int. Acch. Allergy Immunol. v.106 Does amalgam affect the immune system? A controversial issue Enestrom, S.;Hultman, P.
  33. Toxicol. and applied Pharmacol. v.144 Strain Differences in Tissue Concentrations of Mercury in Inbred Mice Treated with Mercuric Chloride Griem, P.;Scholz, E.;Turfeld, M.(et al.)
  34. Biochem. Pharmacol. v.32 Glutathione depletion and in vitro lipid peroxidation in mercury or maleate induced acute renal failure Gstraunthaler, G.;Pfaller, W.;Kotanko, P.
  35. Biochem. Pharmacol. v.45 Studies on Hg(II)-induced $H_2O_2$ formation and oxidative stress in vivo and in vitro in rat kidney mitochondria Lund, B.O.;Miller, D.M.;Woods, J.S.
  36. Journal of Pharmacology & Experimental Therapeutics v.285 no.1 Strain Difference in Sensitivity of Mice to Renal Toxicity of Inorganic Mercury Tanakakagawa, T.;Suzuki, M.Naganuma, A.(et al.)
  37. 기초 독성학 조맹행
  38. Biochemical Pharmacology v.52 Antioxidant Action of Schisandrin B in Protecting Against Carbon Tetrachloride Hepatotoxicity in Mice:A Comparative Study with Butylated Hydroxutoluene Ip, S.P.;Ko, K.M.
  39. Biological & Pharmaceutical Bulletin v.20 no.4 Hepatoprotective Effect of Hovenia Dulcis THUNB on Experimental Liver Injuries Induced by Carbon Tetrachloride or D-Galactosamine/Lipopolysaccharide Hase, K.;Ohsugi, M.;Xiong, Q.B.
  40. Toxicology & Applied Pharmacology v.143 Uptake, Distribution, and Elimination of Carbon Tetrachloride in Rat Tissues Following Inhalation and Ingestion Exposures Sanzgiri, U.Y.;Srivatsan, V.;Muralidhara, S.
  41. Toxicology & Applied Pharmacology v.148 Evaluation of Urinary Biomarkers for Radical-Induced Liver Damage in Rats Treated with Carbon Tetrachloride Dezwart, L.L.;Hermanns, R.C.A.;Meerman, J.H.N.
  42. Hepatology v.28 no.6 Interleukin-10 controls neutrophilic infiltration, hepatocyte proliferation and liver fibrosis induced by carbon tetrachloride in mice Louis, H.;Van Laethem, J.L.;Wu, W.(et al.)
  43. Biological & Pharmaceutical Bulletin v.21 no.10 Effects of dietary unsaturated fatty acid and chronic carbon tetrachloride treatment on the accumulation of oxidation products, alpha-tocopherol and liver injury in mice Yasuda, S.;Watanabe, S.;Kobayashi, T.