The Journal of Pediatrics of Korean Medicine (대한한방소아과학회지)

The Association of Korean Oriental Pediatrics (대한한방소아과학회)
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Purification and Characterization of a new anti-coagulant protein, PP27, of placenta protein (annexinⅤ-like protein)

새로운 인간(人間) 태반(胎盤)유래의 항응고(抗凝固) 단백질(蛋白質) PP27 (annexin Ⅴ형(型) 단백질(蛋白質))의 정제(精製)와 특성(特性)

  • Kim Jang-Hyun (Dept. of Pediatrics, College of Oriental Medicine, Dongguk University)
  • 김장현 (동국대학교 한의과대학 소아과학교실)
  • Published : 2000.12.30
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Abstract

It has long been known that Jahage(紫河車) extracts of Placenta hominis are effective for immunological and vascular diseases in human body and thus, was used a major constituent of traditional oriental medicines. From full-term human placenta, we have purified a new type anticoagulant protein, PP27, using different chromatographic techniques of a phenyl TSK gel 650M column, DEAE, HA and Mono-Q columns. PP27 showed single band on SDS-PAGE with a molecular mass (Mr) of 27 kDa under denaturing conditions and a calibrated Sepharose 4B column chromatography indicated a molecular mass of 23 kDa, indicating that the value is similar to those of other PP4 enzyme reported to date. Isoelectric point of PP27 was p15.2. The protein was found to inhibit the coagulation time in a concentration-dependent manner. PP27 was acted as a vascular anticoagulant of annexin type, inhibits the blood clotting process by binding of the essential lipids in a reaction which is dependent on $Ca2^+$ ions. In the presence of $Ca2^+$ ions, PP27 combines with platelet membranes neutralizing their procoagulant effect. Coagulation triggered by the addition of thromboplastin/ lipid- mixtures is extinguished by PP27.

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References

  1. 本草學 全國韓醫科大學本草學敎室
  2. 申氏本草學 申佶求
  3. 漢藥臨床應用 李尙仁
  4. 中藥大辭典 江蘇新醫學院
  5. 中藥學 高學敏
  6. Biochemistry v.27 $Ca2^+$-dependent phospholipid-(and membrane-) binding proteins Klee, C.B.
  7. Biochem. Biophys. Res. Comm. v.146 Isolation and characterization of tree froms of 36-kDa $ca2^+S- dependent actin-and phospholipid-binding proteins from human placenta membrane Hayashi, H.;Sonbe, S.;Owada, M.K.;Kakunaga, T.
  8. Biochemistry v.27 Placental anticoagulant proteins: isolation and comparative characterization of four members of the lipoprotein family Tait, J.F.;Sakata,M.;McMullen, B.A.;Miao, C.H.;Funakoshi, T.;Hendrickson, L.E.;Fujikawa, K.
  9. J. Biol. Chem. v.263 Five distinct calcium and phospholipid binding proteins share homology with lipocortin I. Pepkinsky, R.B.;Tizard, R.;Mattaliano, R.J.;Sinclair, L.K.;Miller, G.T.;Browning, J. L.;Chow, E.P.;Burne, C.;Huang, K.S.;Pratt, D.;Wachter, L.;Hession, C.;Frey, A.Z.;Wallner, B.P.
  10. Biol. Chem. Hoppe-Seyler v.5 Purification and characterization of six annexins from human placenta Romisch, J.;Heimburger, N.
  11. Eur. J. Biochem. v.151 Isolation and partial purification of a novel anticoagulant from arteries of human umbilical cord Reutelingsperger, C.P.M.;Hornstra, G.;Hemker, H.C.
  12. Throm. Res. v.48 Inhibition of human factor VII a-tissue factor activity by placental anticoagulant protein Kondo, S.;Noguchi, M.;Funakoshi, T.;Fujikawa, K.;Kisiel, W.
  13. J. Biol. Chem. v.264 Phospholipid binding properties of human placental anticoagulant protein-I a member of this lipocortin family Tait, J.F.;Gibson, D.;Fujikawa, K.
  14. Biochim. Biophys. Acta. v.57 Changes in membrane phospholipids during platelet activation Bevers, E.M.;Compurius, P.;Zwaal, R.F.A.
  15. Thrombosis Res. v.60 Anticoagulant properties of placenta protein 4 (Annexin V) Romisch, J.;Schorlemmer, U.;Fickenscher, K.;Paques, E.P.;Heimberger, N.
  16. Thromb. Res. v.50 Phosphorylation of the outer surface of platelets enhances the effects of collagen on aggregation, ATP release, calcium translocation and phosphoinositide hydrolysis Chiang, T.M.;Wojcikiewicz, R.J.H.;Kang, A.H.;Fain, J.N.
  17. Nature v.227 Laemmli, U.K.
  18. J. Biol. Chem. v.193 Lowry, O.H.;Rosenbrough, N.H.;Farr, A.L.;Randall, H.
  19. J. Biochem. v.102 Structure and expression of cDNA for an inhibitor of blood coagulation from human placenta: a new lipocortin-like protein Iwasaki, A.;Suda, M.;Nakakao, H.;Nagota, T.;Saino, Y.;Arai, K.;Mizoguchi, T.;Sato, F.;Yoshizaki, H.;Hirata, M.;Murata, M.;Maki, M.
  20. Carcino Embryonic Proteins v.1 Placental and pregnancy proteins Bohn, H.;Lehmann, F.G.(ed.)
  21. J. Lab. Clin. Med. v.115 Placental anticoagulant protein-IL measurement in extracellular fluids and cells of the hemostatic system Flaherty, M.J.;West, S.;Heima, R.L.Fujikawa, K.;Tait, J.F.
  22. J. Biol. Chem. v.264 Characterizations of two distinct $Ca2^+$-dependent phopholipid-binding proteins of 68-kDa isolated from human placenta Hayashi, H.;Owada, M.;Sonobe, S.;Kakunaga, T.
  23. Proc. Natl. Acad. Sci. v.86 Calcium channel activity of purified human synexin and structure of the human synexin gene Burns. A.;Magendzo, K.;Shirvan, A.;Srivastava, M.;Rojas, E.;Alijani, M.R.;Pollard, H.B.
  24. Br. J. Pharmacol. v.94 Lipocortin and the mechanism of action of the glucocorticoids Flower, R.J.