Herbal Formula Science (대한한의학방제학회지)
- Volume 8 Issue 1
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- Pages.319-328
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- 2000
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- 1229-1218(pISSN)
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- 2288-5641(eISSN)
Effects of Baepungtang water extract on Cultured Spinal Sensory neurons Damaged by Xanthine Oxidase/Hypoxanthine
배풍탕(排風湯) 전탕액(煎湯液)이 XO/HX에 의해 손상(損傷)된 배양(培養) 척수감각신경세포(脊髓感覺神經細胞)에 미치는 효과(效果)
Abstract
To evaluate the effect of Baepungtang(BPT) water extract on cultured mouse spinal sensory neuron which was inhibited by xanthine oxidase(XO) and hypoxanthine(HX)-induced oxigen radicals, MTT assay, NR assay, Neurofilament enzymeimmuno assay and LDH activity assay were carried out after the cultured mouse spinal sensory neuron were preincubated with various concentrations of BPT water extract for 3 hours prior to exposure of XO/HX. The results obtained were as follows: 1. XO/HX, a oxigen radical, decreased the survival rate of the cultured mouse spinal sensory neuron cell on NR assay and MTT assay. 2.
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