Journal of the Korean Association of Oral and Maxillofacial Surgeons
- Volume 26 Issue 3
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- Pages.254-261
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- 2000
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- 2234-7550(pISSN)
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- 2234-5930(eISSN)
EFFECTS OF SIGNAL TRANSDUCTION PATHWAY IN THE RAS-INDUCED CELLULAR TRANSFORMATION OF HUMAN EPITHELIAL CELLS IN CULTURE
인체 상피세포에서 ras-종양유전자의 발암화가 신호 전달 기작에 미치는 영향
- Jang, Do-Geun (Department of Oral & Maxillofacial Surgery, College of Dentistry, Kyungpook National University) ;
- Byeon, Ki-Jeong (Department of Dentistry, Ulsan University Hospital) ;
- Kim, Chin-Soo (Department of Oral & Maxillofacial Surgery, College of Dentistry, Kyungpook National University)
- Published : 2000.06.30
Abstract
The present study has attempted to look into the mechanism of ras-induced carcinogenesis in a human epithelial cell system. Human epithelial cells immortalized with Ad12-SV40 hybrid virus were used to assess carcinogenic potential of the ras-oncogene. Cells transfected with pSV2-ras showed characteristics of cellular transformation. The transformation parameters such as cell density, soft-agar colony formation, and cell aggregation were significantly increased in the cells expressing ras oncoprotein. In addition, the duration required for the appearance of foci was shortened in the ras-transfected cells. Consistent with other reports, our results demonstrated an evidence that the ras-oncogene induced the cellular transformation of human epithelial cell system. When a high concentration of glucocorticoid was added into the media, transformation process was accelerated. It is speculated that glucocorticoid may provide an advantageous environment for the proliferation of the transformed cells. The induction of the intracellular free calcium concentrations following agonist treatment was significantly lower in the transformed cells than in the control cells. These effects were more manifested in the presence of extracellular cacium, indicating that the transformation process may alter the influx pathway of extracellular calcium. The induction of
본 연구는 인체암 발생과 밀접한 관련을 가지고 있는 ras 종양 유전자의 발암화기전을 인체상피세포모델을 이용하여 규명하고자 SV40-Ad12 hybrid virus에 의해 불멸화된 인체상피세포모델에 H-ras 종양유전자을 함유하는
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