Advances in Traditional Medicine
- Volume 1 Issue 1
- /
- Pages.64-70
- /
- 2000
- /
- 2662-4052(pISSN)
- /
- 2662-4060(eISSN)
Effect of Rhizoma gastrodiae on glucose oxydase induced neurotoxicity in cultured mouse spinal dorsal root ganglion neurons
-
Park, Seung-Taeck
(Department of Anatomy, Wonkwang University)
;
- Park, Yang-Kyu (Department of Internal Medicine, Wonkwang University) ;
- Park, Jae-Hwang (Department of Emergency, Wonkwang University) ;
- Cho, Kwang-Ho (Department School of Medicine, Wonkwang University) ;
- Ryu, Do-Gon (College of Oriental Medicine, Wonkwang University) ;
-
Jeon, Byung-Hoon
(College of Oriental Medicine, Wonkwang University)
;
- Shin, Min-Kyo (College of Oriental Medicine, Wonkwang University) ;
- Han, Du-Seok (School of Dentistry, Wonkwang University) ;
- Cho, Nam-Su (School of Medicine, Chosun University) ;
- Shin, Dong-Min (School of Medicine, Chosun University)
- Published : 2000.02.29
Abstract
Effects of Rhizoma gastrodiae on glucose oxidase-induced neurotoxicity was investigated in cultured newborn mouse spinal dorsal root ganglion(DRG) neurons that were treated in the media with or without glucose oxidase. In addition, the protective effect of Rhizoma gastrodiae extract against glucose oxidase-induced neurotoxicity was examined. Cytotoxic values were expressed as a percentage of number of living cells by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In this paper, exposure of neurons to glucose oxidase resulted in a significant call death in a dose- and time-dependent manners in DRG neuron cultures. The decrease in cell viability induced by the glucose oxidase was blocked by Rhizoma gastrodiae extract. These results indicate that the neuroprotective effect of Rhizoma gastrodiae extract against glucose oxidase-induced neurotoxicity may result from a prevention or attenuation of oxidative damage induced by glucose oxidase.